Treatment of Scorpion Envenomations in the Middle East: Understanding the Stinging Controversy

Brady, Mark F.; Kumar, Prasanna; Currier, Chase; Ruha, Anne‐Michelle

doi:10.1016/j.wem.2023.01.001

Cited by 3 publications

(1 citation statement)

References 9 publications

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“…According to global health statistics, scorpion stings are a dangerous and neglected public health problem in countries with arid and semi-arid climates, especially in the Middle East 1 . Each year, scorpions cause 1.2 million stings, resulting in 3250 deaths 2 .…”

Section: Introductionmentioning

confidence: 99%

Mass spectrometric analysis of Odonthobuthus Doriae scorpion venom and its non-neutralized fractions after interaction with commercial antivenom

Abdollahnia,

Bahmani,

Aliahmadi

et al. 2024

Sci Rep

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It is believed that antivenoms play a crucial role in neutralizing venoms. However, uncontrolled clinical effects appear in patients stung by scorpions after the injection of antivenom. In this research, non-neutralized components of the venom of the Iranian scorpion Odonthobuthus doriae were analyzed after interacting with the commercial antivenom available in the market. The venom and antivenom interaction was performed, then centrifuged, and the supernatant was analyzed by high-performance liquid chromatography (HPLC). Two peaks of Odonthobuthus doriae venom were observed in the chromatogram of the supernatant. Two components were isolated by HPLC and analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) instruments. Peptide sequencing was done by Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry (LC-Q-TOF MS/MS). Results indicate that the components of scorpion venom mainly have a molecular weight below 10 kDa, consisting of toxic peptides that disrupt the function of sodium and potassium channels. The MALDI-TOF MS results show that two toxic peptides with molecular masses of 6941 Da and 6396 Da were not neutralized by the antivenom. According to the MS/MS sequencing data, the components have been related to peptides A0A5P8U2Q6_MESEU and A0A0U4FP89_ODODO, which belong to the sodium and potassium channels toxins family, respectively.

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Section: Introductionmentioning

confidence: 99%