Treatment of rheumatoid synovitis with anti-reshaping human interleukin-6 receptor monoclonal antibody: Use of rheumatoid arthritis tissue implants in the SCID mouse model

Matsuno, Hiroaki; T, Sawai; Nezuka, T.; Uzuki, Miwa; Tsuji, Haruo; Nishimoto, N.; Yoshizaki, Kazuhito

doi:10.1002/1529-0131(199811)41:11<2014::aid-art17>3.3.co;2-k

Cited by 2 publications

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“…The SCID-HuRAg mouse is considered to be a useful model for the evaluation of biological agents [13]. A previous study has shown that the implanted tissue in SCID-HuRAg could grow one to two times bigger in size by 8 weeks after implantation [14]. In this study, we measured the size of grafted tissues in different groups and found that the volume of grafts reduced significantly in the group treated with anti-CD147 mAb, anti-TNF-mAb and the combined treatment compared with that in the control mAb group, showing that CD147 mAb inhibited the proliferation of RA synovium.…”

Section: Discussionmentioning

confidence: 66%

“…SCID-HuRAg mice were prepared according to previous reports [12][13][14]. Briefly, 6-to 8-week-old male NOD/SCID mice (SLAC, Shanghai Laboratory Animal Co. Ltd, Shanghai, China), which had been bred under specific pathogen-free conditions, were used for the experiments.…”

Section: Preparation Of the Scid-hurag Modelmentioning

confidence: 99%

“…However, most reports of CD147 antagonist on RA are from in vitro studies [6][7][8][9][10] and the anti-mouse mAb used in the in vivo study was a heterogeneous protein to humans [11]. To assess a suppressive effect of CD147/HAb18 mAb (a mouse anti-human CD147 mAb) on the cartilage erosion and synovitis of RA patients in vivo, we conducted a treatment study using severe combined immunodeficiency (SCID) mice, into which rheumatoid synovium and normal cartilage were co-grafted (SCID-HuRAg) [12][13][14]. Systemic and local parameters of RA, including volume of engrafts, inflammatory cell number and cartilage erosion score, as well as expression of MMPs and inflammatory cytokines levels in the serum, were evaluated.…”

Section: Introductionmentioning

confidence: 99%

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Inhibitory effect of CD147/HAb18 monoclonal antibody on cartilage erosion and synovitis in the SCID mouse model for rheumatoid arthritis

et al. 2009

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Section: Discussionmentioning

confidence: 66%

Section: Preparation Of the Scid-hurag Modelmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inhibitory effect of CD147/HAb18 monoclonal antibody on cartilage erosion and synovitis in the SCID mouse model for rheumatoid arthritis

et al. 2009

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Study of the Mechanism Involved in Angiogenesis and Synovial Cell Proliferation in Human Synovial Tissues of Patients with Rheumatoid Arthritis Using SCID Mice

Nagashima

Tanaka

Takahashi

et al. 2002

Laboratory Investigation

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SUMMARY:To examine whether synovial cell proliferation is due to angiogenesis, we studied the relationship between the inhibition of synovial cell proliferation and an angiogenesis inhibitor, TNP-470, in human synovial tissues. Human synovial tissues were implanted into the back of SCID mice (SCID-HuAg mice). Sixteen mice were divided into two groups of eight mice each: the untreated group (vehicle group) and the TNP-470-treated group that received a dose of 10 mg/kg body weight by subcutaneous injection. The number of blood vessels and synovial lining cells clearly increased in the vehicle group, but the number of synovial lining cells clearly decreased and the blood vessels were hardly detected in the TNP-470 group. Immunohistochemically, cells that stained positively for the anti-proliferating cell nuclear antigen (PCNA) mAb were abundant in synovial lining cells and endothelial cells in synovial tissues. Cells that stained positively for the anti-CD34 polyclonal antibody were abundant in the endothelial cells in the vehicle group, but these positively stained cells were hardly detected in the TNP-470 group. The PCNA positivity ratio in the vehicle group was 0.64 Ϯ 0.019, whereas that in the TNP-470 group was 0.199 Ϯ 0.007. The numbers of cells that stained positively for anti-CD34 polyclonal antibody were 242 Ϯ 13.4 in the vehicle group and 153 Ϯ 6.73 in the TNP-470 group per 10 microscopic fields. Cells that stained positively for anti-mouse CD31 mAb were mainly localized in the synovial lining, but invaded the subsynovial lining layer in human synovial tissues. On the other hand, cells that stained positively for anti-human CD31 mAb were mainly localized in the subsynovial lining layer. We found that endothelial cell proliferation is dependent on angiogenesis based on the result that angiogenesis and synovial cell proliferation were inhibited by treatment with TNP-470. (Lab Invest 2002, 82:981-988).

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Treatment of rheumatoid synovitis with anti-reshaping human interleukin-6 receptor monoclonal antibody: Use of rheumatoid arthritis tissue implants in the SCID mouse model

Cited by 2 publications

References 0 publications

Inhibitory effect of CD147/HAb18 monoclonal antibody on cartilage erosion and synovitis in the SCID mouse model for rheumatoid arthritis

Inhibitory effect of CD147/HAb18 monoclonal antibody on cartilage erosion and synovitis in the SCID mouse model for rheumatoid arthritis

Study of the Mechanism Involved in Angiogenesis and Synovial Cell Proliferation in Human Synovial Tissues of Patients with Rheumatoid Arthritis Using SCID Mice

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