Treatment of pulmonary disease caused by opportunist mycobacteria.

Banks, John

doi:10.1136/thx.44.6.449

Cited by 10 publications

(3 citation statements)

References 60 publications

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“…Environmental mycobacteria exhibit great variation in growth rates, colony morphologies and virulence and antibiotic sensitivities [17]. There is no general agreement about the treatment of patients with NTM infections [13,16,18]. In spite of high species diversity we found similar patterns of drug resistance between different NTM species.…”

Section: Discussionmentioning

confidence: 87%

“…This study aims to give a broader view of the identification of NTM infections in humans using the PRA method, which is more accurate and rapid. Identification of species among mycobacteria is valuable because it provides useful information regarding patient management and proper treatment [11–13]. Outbreaks of NTM infections related to contaminated instruments in hospitals have been described all over the world.…”

Section: Discussionmentioning

confidence: 99%

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HSP65-PRA identification of non-tuberculosis mycobacteria from 4892 samples suspicious for mycobacterial infections

Saifi

Jabbarzadeh

Bahrmand

et al. 2013

Clinical Microbiology and Infection

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Various molecular methods have been used for the rapid identification of mycobacterial species. In this survey, evaluation of antibiotic resistance and PCR-restriction fragment length polymorphism analysis (PRA) of the hsp65 gene was carried out for identification of non-tuberculosis mycobacteria (NTM) isolates from different clinical specimens. Forty-eight different mycobacterial isolates were selected and followed by the conventional and PRA of hsp65 for species identification. The antibiotic susceptibility test was carried out according to standard methods. A 439 bp PCR product of hsp65 in all selected isolates was amplified and digested with the BstEII and HaeIII restriction enzymes. The restriction fragment length polymorphism (RFLP) patterns were analyzed for species identification. Using PRA for 48 mycobacterial selected isolates, including 15 M. tuberculosis, one M. bovis and all 32 isolates of NTM, revealed 11 different species among the NTM isolates. The most frequent NTM isolates were M. kansasii, M. gordonae III, M. marinum, M. chelonae, M. scrofluaceum and M. gastri. In most cases, the PRA results were perfectly in accordance with the classical biochemical method. Combination of resistance to rifampin and isoniazid was present among M. kansasi, M. gordoniae III, M. scrofluaceum, M. chelonae, M. marinum, M. gastri, M. gordoniae II and M. trivale isolates. A high incidence of co-resistance to six, five, four and three anti-TB drugs was observed in 18.5%, 9.1%, 6.6% and 11.7% of all NTM isolates, respectively. Our results showed that PRA, in comparison with classical methods, is rapid and accurate enough for the identification of mycobacterial species from LJ medium. Additionally, we found that in Iran we have a highly diverse population of NTM isolates among patients suspected of having TB.

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

HSP65-PRA identification of non-tuberculosis mycobacteria from 4892 samples suspicious for mycobacterial infections

Saifi

Jabbarzadeh

Bahrmand

et al. 2013

Clinical Microbiology and Infection

View full text Add to dashboard Cite

show abstract

“…From a prognostic point of view, such NTM disease indicates a debilitating underlying immune deficiency, which can make management difficult. 19 This presentation is commonly caused by MAC but other species, such as M. kansasii and M. chelonae, have been reported to cause similar illness.…”

Section: Bacteriologicalmentioning

confidence: 99%