Treatment of postoperative otorrhoea by grafting of mastoid cavities with cultured autologous epidermal cells

Premachandra, D. J.; Milton, Catherine M.; Sergeant, R. J.; Woodward, B.; Fabre, John W.

doi:10.1016/0140-6736(90)90204-i

Cited by 39 publications

(14 citation statements)

References 16 publications

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“…* A new technique for improving discharging mastoid cavities was described by Premachandra in 1Y90. 9 Hc reported initially encouraging results by grafting of mastoid cavities with cultured autologous epidermal cells, thereby promoting epithelialization in areas previously lined by ulcerated granulation tissue.…”

mentioning

confidence: 99%

The histopathology of mastoidectomy cavities, with particular reference to persistent disease leading to chronic otorrhoea

Youngs

1992

Clin Otolaryngol

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Open cavity mastoidectomy remains the principal surgical treatment of middle ear cholesteatoma in the United Kingdom. A significant proportion of mastoid cavities are prone to intermittent or continuous discharge. In this study the histopathological features of material removed from 159 mastoid cavities at revision surgery were reviewed. Findings included squamous epithelium with acute and chronic inflammation, foreign body granuloma and aural polyps. Of particular note was the very infrequent finding of discharging cavities lined with respiratory epithelium, suggesting that retained mucosa in mastoid air cells is not a common cause of persistent otorrhoea.

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mentioning

confidence: 99%

The histopathology of mastoidectomy cavities, with particular reference to persistent disease leading to chronic otorrhoea

Youngs

1992

Clin Otolaryngol

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“…Normally it requires 3 weeks to expand a 2 cm 2 biopsy to a surface, sufficient to cover the whole body‐surface (1·73 m 2 ). Some studies showed that culturing skin of the elderly (>60 years) takes still more time to reach confluence (25,31).…”

Section: Current Use Of Keratinocytesmentioning

confidence: 99%

Keratinocytes in the treatment of severe burn injury: an update

Lootens

Brusselaers²,

Beele

et al. 2012

International Wound Journal

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Burns are among the most life-threatening physical injuries, in which fast wound closure is crucial. The surgical burn care has evolved considerably throughout the past decennia resulting in a shift of therapeutic goals. Therapies aiming to provide coverage of the burn have been replaced by treatments that have both functional as aesthetic outcomes. The standard in treating severe burns is still early excision followed by skin grafting. The use of cultured keratinocytes to cover extensive burn wounds appeared very promising at first, but the technique still has several limitations of which the long time to culture, the major costs, the risk of infection and the need for an adequate dermal layer limit clinical application. The introduction of dermal substitutes, composite grafts, tissue engineering based on stem cell application have been advocated. The aim of this review is to assess the use of cultured keratinocytes in terms of technical aspects, clinical application, limitations and future perspectives. Cultured keratinocytes are expected to keep playing a role in wound healing, especially in the field of chronic wounds. In severe burns, despite its limitations, keratinocytes can be beneficial if implemented as one of the elements in a broader wound management.

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“…46 and other miscellaneous problems that require wound closure. 47 The advantage of this technique is that a small biopsy specimen of skin can be expanded 10 000-fold in 3 weeks, theoretically providing enough epidermal sheets to cover the entire body surface area and thus avoid creating a large donor site wound.48 Enthusi¬ asm for their clinical use quickly developed49 and in 1989, cultured keratinocyte autografts became commercially avail¬ able. Their use has now become a growth industry, with more than 240 patients in the United States grafted with commercially obtained material by 1992.…”

Section: Characteristics and Developmentmentioning

confidence: 99%

Skin Replacements

Cairns

deSerres²,

Peterson³

et al. 1993

Arch Surg

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Extensive skin loss from a variety of conditions is associated with significant functional morbidity and loss of life. In many patients, a limited number of donor sites available for harvesting autologous split-thickness skin grafts prevents early, effective, and permanent wound closure. In the past 25 years, significant biotechnological advancements have been made in defining the criteria and manufacturing ingredients in materials that could serve as skin replacements for permanent wound closure. The optimal skin replacement should have the functional and cosmetic properties of the dermis and the epidermis. It should provide rapid, functional wound coverage and barrier protection to microorganisms, normalize fluid flux and hypermetabolism, and provide long-term stability without contraction or hypertrophic scarring. In addition, the optimal skin replacement should be nontoxic, easily stored and used, and relatively cost-effective. This report will discuss the two major skin replacement designs available today, cultured keratinocyte grafts and bioartificial bilaminate systems, outline the advantages and disadvantages of each material, report the results of clinical trials for each, and speculate on the potential for each material to serve as a practical skin replacement.

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Treatment of postoperative otorrhoea by grafting of mastoid cavities with cultured autologous epidermal cells

Cited by 39 publications

References 16 publications

The histopathology of mastoidectomy cavities, with particular reference to persistent disease leading to chronic otorrhoea

The histopathology of mastoidectomy cavities, with particular reference to persistent disease leading to chronic otorrhoea

Keratinocytes in the treatment of severe burn injury: an update

Skin Replacements

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