Treatment of porcine ovarian follicles with tert-butyl hydroperoxide as an ovarian senescence model in vitro

Abstract

Ovarian aging is the main reason of female reproductive problems. Excessive oxidative stress can induce ovarian senescence and follicular atresia, thereby reducing the reproductive performance. Follicles were divided into five groups for in vitro culture based on the duration of stimulation with tert-butyl hydroperoxide (t-BHP)—control group and groups 1 h, 2 h, 6 h, and 12 h. The results revealed that the ratio of progesterone (P 4 ) to estradiol (E 2 ) was increased after 24 and 36 h of follicle culture, shifting follicles toward atresia ( P < 0.05). Stimulated by 200 μM t-BHP, follicles showed progressive aging phenotype. Senescence-associated β-galactosidase staining (SA-β-Gal) showed a significant increase in the number of positive cells ( P < 0.05). Reactive oxygen species were also significantly upregulated ( P < 0.05). t-BHP treatment for 6 h induced significant increases in Caspase 3, P53, and Foxo1 mRNA and protein levels ( P < 0.05) and significant decreases in SOD mRNA and protein levels ( P < 0.05). Transcriptome sequencing analysis of the follicles showed that the aged and treatment groups were clustered together in hierarchical clustering. Correlation analysis indicated significant changes at the transcriptome level in the treatment groups versus the control group. The common differentially expressed genes in the treatment groups were enriched in three growth-factor signaling pathways associated with cell proliferation and apoptosis (P53, mTOR, and MAPK). In conclusion, induction of follicular senescence by treatment with 200 μM t-BHP for 6 h is an effective in vitro model to simulate ovarian senescence in sows.

CBLL1 promotes endometrial stromal cell senescence via inhibiting PTEN in recurrent spontaneous abortion

“…tert-Butyl hydroperoxide (TBHP) (Macklin, China) was used to induce cellular senescence of HESCs. 17,18 HESCs were treated with 0 μM (control), 50, 100, and 200 μM TBHP for 1 h. Subsequently, cells were washed twice with PBS to remove any residual TBHP and then cultured in fresh complete medium for at least 14 days until cells reached irreversible senescence. 9,17…”