Treatment of Paper for Chromatography of some Colloidal Electrolytes

Rutter, L.

doi:10.1038/166273a0

Cited by 13 publications

(4 citation statements)

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“…(Hauser and Lynn, 1940), " cetab " (Rutter, 1950), and albumin (Tiselius, 1953). Jermyn and Thomas (1953) The procedure commonly adopted is to remove the paper strip after completion of the electrophoretic run, taking care in removing it not to allow it to brush against the apparatus or the bench.…”

Section: Filter-paper Electrophoresismentioning

confidence: 99%

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The Use and Limitations of Filter-paper Electrophoresis

Martin¹,

Franglen²

1954

Journal of Clinical Pathology

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In 1935 Tiselius, using the apparatus he had devised in 1930, demonstrated that human serum globulin fell into three reasonably defined groups based on their electrophoretic mobilities. For the next 15 years a steady flood of papers reported the fluctuations of albumin and the globulins in young and old, in health and disease, as disclosed by analyses carried out using Tiselius apparatus with and without the numerous minor modifications which were introduced.In spite of brilliant efforts at simplification it was evident that the method, because of its relative complexity, could never have widespread use in routine clinical chemistry. In 1950 three groups of workers, among them Tiselius himself, described methods which indicated that, using filter paper as a matrix, a relatively cheap and rapid method could be evolved of obtaining a rough analysis of the serum proteins akin to the analysis obtained by the classical technique.The method required no more than 0.5 ml. of serum, and though estimates of the total protein concentration would still have to be made by other means, it was conceivable that this simple technique would dispense with the tedious salt fractionation and subsequent analyses required heretofore to obtain the albumin/globulin ratio.The last four years have produced a spate of papers devoted to the description of modifications in methods of separation and analysis with numerous technical and mathematical tricks for improving the correlation of the results with the substantial body of data already obtained with the classical technique. With few notable exceptions scant attention was paid to the fundamental principles underlying the technique or to the points in which their application to it differed from the classical procedure.As paper succeeded paper it became increasingly evident that except within fairly wide limits the two methods were not quantitatively comparable.Even worse, because of lack of time spent on a consideration of the basic principles involved, G it seemed probable that differing techniques for the production of " electrophoretograms " were not comparable among themselves.One was faced with the prospect of scores of laboratories turning out hundreds of results year by year; each group, because of deviations in technique unappreciated and therefore unrecorded, differing to an unknown extent one from the other. The clinical chemical chaos that could arise demanded a reassessment of the situation in the hope that workers in the field would stop, think, and start again.Electrokinetic phenomena, as the derivation of the word implies, are concerned with the

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Section: Filter-paper Electrophoresismentioning

confidence: 99%

“…It carries a measurable negative charge. Many attempts have been made to neutralize this charge using gentian violet (Hauser and Lynn, 1940), " cetab " (Rutter, 1950), and albumin (Tiselius, 1953). Jermyn and Thomas (1953) have studied the endo-osmotic flow using spots of tri-nitro benzene and 0. nitroaniline.…”

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confidence: 99%

The Use and Limitations of Filter-paper Electrophoresis

Martin¹,

Franglen²

1954

Journal of Clinical Pathology

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“…It may also be supported between glass plates which permit use at any angle and which prevent evaporation of the wash liquid (Figure 3). It may be utilized as a support for more active adsorbents (58,91,127,218,228,337). Substances resolved on paper are readily recoverable (238).…”

Section: Chromatographic Apparatusmentioning

confidence: 99%

Chromatographic Systems

Strain¹

1951

Anal. Chem.

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“…Identification and Exa11zination.-The various reports on methods of identifying and separating textile fibers and fiber!mixtures are both numerous and diversified [1178,1187,1188,1191,1196,1197,1203,1213]. Chromatographic techniques have had wide application to problems in textile research and development [1181,1183,1186,1192,1195,1202,1212,1214]. Phase-contrast microscopy, a relatively old technique, has found increasing application to the study of textiles [1185,1198,1199] and augments s the conventional methods of microscopy [1179,1193,1200,1201,1208,1210,1211 ] .…”

Section: Control Instruments Andmentioning

confidence: 99%

Review of Textile Research and Development During 1950

Steele¹,

Wakeham²,

Wakelin³

et al. 1951

Textile Research Journal

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This paper is a review of the literature published during 1950 in the field of textile science and technology. The breadth of the subject matter in pure science and in technology represented by the extensive bibliography indicates the many important fields in which the textile industry is now devoting effort and which it must follow closely if improvements in processing operations and in materials are to be translated into more useful products for the con-sumer.Papers in the field of research have been concerned with studies of the structure and properties of natural and synthetic fibers, with the chemistry of cellulose and starch, with the microbiological degradation of cellulose and wool, and with the processes of adsorption, swelling, diffusion, dyeing, and detergency. Certain general papers in the field of high polymers have been included because the techniques are important in textile research. Methods for determining molecular weight by viscometry, osmotic pressure, light scattering, and centrifugation have been reviewed. New equipments and techniques for the control of processing and laboratory equipment and for the test, identification, and examination of textile materials have been considered.In the field of development, the papers in the literature have reported on improved processes for opening and blending, carding, drafting, spinning, winding, weaving, and knitting. In wet processing attention has been given to scouring, sizing, dyeing, and printing, bleaching, and stripping. The literature on finishing includes studies of dimensional stabilization of fabrics, flame-resistance, waterproofing, resin finishes, and coating materials.II. Introduction ' The material reviewed in this paper has been selected from the scientific and technical journals published in the United States and abroad, and from certain standard abstract journals. It is believed that this review covers the important contributions in the fields of textile research and development during 1950; however, certain papers assembled at the outset have been omitted because they were considered inappropriate. The material in the text has been divided somewhat arbitrarily into the fields of research and development, and into sub-classifications of these divisions controlled principally by the published literature.The bibliography has been arranged to conform with the main divisions and their sub-classifications. The individual papers cited are numbered consecutively in the bibliography; the papers in each of the principal sections in research and development are arranged in alphabetical order by first author. The numbers in brackets in the text refer to the appropriate papers in the bibliography. III. Review Papers of General InterestThe general literature on textiles during the past year includes the publication of many books and scientific papers which are not concerned with particular materials or processes but deal in a broad way with the textile field. These publications treat such subjects as the general progress in research and develop...

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Treatment of Paper for Chromatography of some Colloidal Electrolytes

Cited by 13 publications

References 2 publications

The Use and Limitations of Filter-paper Electrophoresis

The Use and Limitations of Filter-paper Electrophoresis

Chromatographic Systems

Review of Textile Research and Development During 1950

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