Treatment of Nitrogen Heterocyclic Compounds (NHCs) in Coking Wastewater by White-Rot Fungi

Background In the search for methods to biodegrade recalcitrant compounds, the use of saprotrophic fungi and white rot fungi, in particular belonging to the phylum Basidiomycota, has gained interest. This group of fungi possesses a battery of unspecific extracellular enzymes that can be utilized in the biodegradation of preferably phenolic compounds. In this work, it was investigated under which conditions the white rot fungus Trametes versicolor and the ericoid mycorrhizal fungus Rhizoscyphus ericae (belonging to the phylum Ascomycota) could be used to biodegrade the antibiotic aminoglycoside neomycin at co-metabolic conditions in which external nutrients were supplied. Furthermore, it was also investigated whether a biodegradation could be accomplished using neomycin as the sole nutrient. Results The results show that both species can biodegrade neomycin 70% under co-metabolic conditions during a one-week time course and that Rhizoscyphus ericae is able to use neomycin as sole nutrient and to approximatively biodegrade it 60% under chosen non co-metabolic conditions. At selected conditions, the biodegradation of neomycin using Rhizoscyphus ericae was monitored by oxidation products of D-ribose which is a hydrolysis product of neomycin. Conclusion The results are of general interest in the search for fungal species that can biodegrade recalcitrant compounds without the need of external nutrients. The key future application area that will be investigated is purification of waste from recombinant protein production in which neomycin, nutrients and E. coli with neomycin resistance genes are present.

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“…When the target substance is present at a high concentration level, the reaction can obey zero order kinetics [ 32 ]. See Eq.…”

Section: Methodsmentioning

confidence: 99%

Investigation of neomycin biodegradation conditions using ericoid mycorrhizal and white rot fungal species

2022

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“…The effect of MgSO 4 •7H 2 O on sulfide oxidation could be interpreted differently compared to other reagents in the CM because it had a negligible impact on ligninolytic enzyme secretion (Figure 2d) and sulfide oxidation (Figure 3d) in the evaluated interval. Even though the concentration of Mg has been reported to favor fungal growth [32], it seems that the concentration of yeast extract and malt extract in the evaluated CM could have been sufficient to provide the Mg content and other TE required for fungal growth [30,31].…”

Section: Effect Of CM On Sulfide Bio-oxidationmentioning

confidence: 99%

Assessment of Modified Culture Conditions for Fungal Bio-Oxidation of Sulfidic Gold Ores Performed at Circumneutral pH

Hein,

Mahandra,

Ghahreman

2023

Sustainability

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The significant neutralization of waste streams required after the acidic bio-oxidation of sulfidic gold ores could be avoided by performing a novel treatment at circumneutral pH with an in situ neutralization. For the first time, the white-rot fungus Phanerochaete chrysosporium was incubated in a modified culture medium containing corn steep, an industrial waste product, to support microbial activity and, subsequently, the oxidation of a sulfidic ore at an initial circumneutral pH environment. In this investigation, the concentration of the native culture medium ingredients was first evaluated with response surface methodology to attain maximum sulfide oxidation. The statistical analysis proposed a modified culture medium composed of 12.86 g/L glucose, 2.20 g/L malt extract, 1.67 g/L yeast extract, and 0.49 g/L MgSO4·7H2O to reach a maximum of 28.7% sulfide oxidation after 14 d-bio-oxidation. pH-controlled batch cultures showed that an increase in initial pH in the range of 5.8 to 7.0 reduced the microbial activity, affecting sulfide oxidation. In addition, the modified culture medium at which yeast extract was substituted with 1.67 g/L corn steep produced comparable microbial activity and sulfide oxidation after 14 d, attaining 21.6% at 5% w/v with a maximum 39 U/L lignin peroxidase and 116 U/L manganese peroxidase. A 40.6% sulfide oxidation and 43.8% gold recovery were obtained after 42 d three-cycle replenishing bio-oxidation and 24 h cyanidation, respectively. Overall, corn steep waste showed the potential to substitute more expensive culture medium ingredients, supporting microbial activity and oxidation of sulfidic gold ores at an initial circumneutral pH and contributing to circularity of waste management.

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“…When the target substance is present at a high concentration level, the reaction can obey zero order kinetics (Huang et al 2019). See equation (3).…”

Section: ) See Equation (2)mentioning

confidence: 99%

Investigation of neomycin biodegradation conditions using ericoid mycorrhizal and white rot fungal species

Stenholm

Hedeland

Pettersson

2022

Preprint

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In the search for methods to biodegrade recalcitrant compounds, the use of saprophytic fungi and white rot fungi, in particular belonging to the phylum Basidiomycota, has gained interest. This group of fungi possesses a battery of unspecific extracellular enzymes that can be utilized in the biodegradation of preferably phenolic compounds. In this work, it was investigated under which conditions the white rot fungus Trametes versicolor and the ericoid mycorrhizal fungus Rhizoscyphus ericae (belonging to the phylum Ascomycota) could be used to biodegrade the antibiotic aminoglycoside neomycin at co-metabolic conditions in which external nutrients were supplied. Furthermore, it was also investigated whether a biodegradation could be accomplished using neomycin as the sole nutrient. At selected conditions, the biodegradation of neomycin using Rhizoscyphus ericae was monitored by oxidation products of D-ribose which is a hydrolysis product of neomycin. The results show that both species can biodegrade neomycin 70% under co-metabolic conditions during an one-week time course and that Rhizoscyphus ericae is able to use neomycin as sole nutrient and to approximatively biodegrade it 60% under chosen non co-metabolic conditions. The results are of general interest in the search for fungal species that can biodegrade recalcitrant compounds without the need of external nutrients. The key future application area that will be investigated is purification of waste from recombinant protein production in which neomycin, nutrients and E. coli with neomycin resistance genes are present.

show abstract

Treatment of Nitrogen Heterocyclic Compounds (NHCs) in Coking Wastewater by White-Rot Fungi

Cited by 6 publications

References 17 publications

Investigation of neomycin biodegradation conditions using ericoid mycorrhizal and white rot fungal species

Investigation of neomycin biodegradation conditions using ericoid mycorrhizal and white rot fungal species

Assessment of Modified Culture Conditions for Fungal Bio-Oxidation of Sulfidic Gold Ores Performed at Circumneutral pH

Investigation of neomycin biodegradation conditions using ericoid mycorrhizal and white rot fungal species

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