Treatment of MRSA-infected osteomyelitis using bacterial capturing, magnetically targeted composites with microwave-assisted bacterial killing

Qiao, Yuqian; Liu, Xiangmei; Li, Bo; Han, Yong; Zheng, Yufeng; Yeung, Kelvin Wai Kwok; Li, Changyi; Cui, Zhenduo; Liang, Yanqin; Li, Zhaoyang; Zhu, Shengli; Wang, Xiaochang C.; Wu, S. L.

doi:10.1038/s41467-020-18268-0

Cited by 193 publications

(137 citation statements)

References 41 publications

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“…Studies have reported that prussian blue nanoparticles synthesized as photosensitive hydrogels exhibiting strong photothermal properties under NIR irradiation resulted in the capture of bacteria in hydrogels and its further killing by perturbation of bacterial cell membranes in S. aureus (99.97%) and E. coli (99.93%) (Han et al, 2020b). Similar results were observed in MRSA-infected osteomyelitis to treat tissue infections using magnetically iron oxide-targeted carbon nanotubes (Fe 2 O 3 /CNT), resulting in microwave-assisted bacterial killing (Qiao et al, 2020). However, more studies are needed to further reduce the cytotoxicities of PANs.…”

Section: Controlled Release Of Photothermal Agentsmentioning

confidence: 61%

Combating Drug-Resistant Bacteria Using Photothermally Active Nanomaterials: A Perspective Review

et al. 2021

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Injudicious use of antibiotics has been the main driver of severe bacterial non-susceptibility to commonly available antibiotics (known as drug resistance or antimicrobial resistance), a global threat to human health and healthcare. There is an increase in the incidence and levels of resistance to antibacterial drugs not only in nosocomial settings but also in community ones. The drying pipeline of new and effective antibiotics has further worsened the situation and is leading to a potentially “post-antibiotic era.” This requires novel and effective therapies and therapeutic agents for combating drug-resistant pathogenic microbes. Nanomaterials are emerging as potent antimicrobial agents with both bactericidal and potentiating effects reported against drug-resistant microbes. Among them, the photothermally active nanomaterials (PANs) are gaining attention for their broad-spectrum antibacterial potencies driven mainly by the photothermal effect, which is characterized by the conversion of absorbed photon energy into heat energy by the PANs. The current review capitalizes on the importance of using PANs as an effective approach for overcoming bacterial resistance to drugs. Various PANs leveraging broad-spectrum therapeutic antibacterial (both bactericidal and synergistic) potentials against drug-resistant pathogens have been discussed. The review also provides deeper mechanistic insights into the mechanisms of the action of PANs against a variety of drug-resistant pathogens with a critical evaluation of efflux pumps, cell membrane permeability, biofilm, and quorum sensing inhibition. We also discuss the use of PANs as drug carriers. This review also discusses possible cytotoxicities related to the therapeutic use of PANs and effective strategies to overcome this. Recent developments, success stories, challenges, and prospects are also presented.

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Section: Controlled Release Of Photothermal Agentsmentioning

confidence: 61%

Combating Drug-Resistant Bacteria Using Photothermally Active Nanomaterials: A Perspective Review

et al. 2021

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“…Combining the magnetic targeting and MCT, Wu et al constructed a dual-targeting and MV-responsive nanocarriers for precise and synergistic therapy for combating MRSA-induced osteomyelitis (Fig. 12 ) [ 73 ]. Briefly, mesoporous iron oxide NPs were modified with dielectric carbon nanotubes (CNTs) with good MV absorption ability to obtain neural-network-like Fe 3 O 4 /CNT.…”

Section: Nanocarriers Responsive To External Stimulimentioning

confidence: 99%

Stimuli-responsive nanocarriers for bacterial biofilm treatment

et al. 2021

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Bacterial biofilm infections have been threatening the human’s life and health globally for a long time because they typically cause chronic and persistent infections. Traditional antibiotic therapies can hardly eradicate biofilms in many cases, as biofilms always form a robust fortress for pathogens inside, inhibiting the penetration of drugs. To address the issues, many novel drug carriers emerged as promising strategies for biofilm treatment. Among them, stimuli-responsive nanocarriers have attracted much attentions for their intriguing physicochemical properties, such as tunable size, shape and surface chemistry, especially smart drug release characteristic. Based on the microenvironmental difference between biofilm infection sites and normal tissue, many stimuli, such as bacterial products accumulating in biofilms (enzymes, glutathione, etc.), lower pH and higher H 2 O 2 levels, have been employed and proved in favor of “on-demand” drug release for biofilm elimination. Additionally, external stimuli including light, heat, microwave and magnetic fields are also able to control the drug releasing behavior artificially. In this review, we summarized recent advances in stimuli-responsive nanocarriers for combating biofilm infections, and mainly, focusing on the different stimuli that trigger the drug release. 摘要细菌生物膜常造成长期, 顽固的感染, 因此长久以来都严重威胁着全球人类的生命健康。由于生物膜会为内部病原体建造其保护性屏障, 阻碍药物的渗透, 因此传统的抗生素疗法通常难以根除生物膜。为了解决这一问题, 研究人员构建了许多新型的药物载体, 以作为颇具前景的生物膜治疗策略。其中, 刺激响应型纳米载体具有有趣的物理-化学性质, 例如尺寸, 形状和表面化学可调节的性质, 特别是智能药物释放性质, 因此吸引了人们的广泛关注。根据生物膜感染位置和正常组织之间的微环境差异, 人们利用多种刺激物, 比如生物膜内积累的细菌产物 (酶, 谷胱甘肽等), 低pH和高H 2 O 2 水平, 来设计药物的“按需释放”以清除生物膜。此外, 包括光, 热, 微波和磁场在内的外源刺激, 也可以用于人工控制药物的释放行为。本篇综述中, 我们总结了刺激响应型纳米载体在对抗细菌生物膜中的最新进展, 并着重于引发药物释放的多种刺激物。 Graphic abstract

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“…Osteomyelitis is a difficult-to-treat bone disease often caused by a bacterial infection following a fracture or surgery. (1). It has been reported that up to 30% of high-risk open fractures will be infected, which could progress to organ infection, bone destruction, and sepsis, and is life-threatening (2,3).…”

Section: Introductionmentioning

confidence: 99%

Emodin Combined with Multiple Low-frequency Low-intensity Ultrasound to Relieve Osteomyelitis Through Sonoantimicrobial Chemotherapy

et al. 2022

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Treatment of osteomyelitis is still challenging as conventional antibiotic therapy is limited by the emergence of resistant strains and the formation of biofilms. Sonoantimicrobial chemotherapy (SACT) is a novel therapy of low-frequency and low-intensity ultrasound (LFLIU) combined with sonosensitizer. Therefore, in our study, a sonosensitizer named emodin (EM) was proposed to be combined with LFLIU to relieve acute osteomyelitis caused by Methicillin-resistant Staphylococcus aureus (MRSA) through synergistic antibacterial and anti-biofilm effects. The efficiency of different intensities of ultrasound, single (S-LFLIU, 15 min) and multiple ultrasound (M-LFLIU, 5 min every 4 h, three times) against bacteria and biofilm was compared, contributing to develop the best treatment regimen. Our results demonstrated that EM plus S-LFLIU or M-LFLIU (EM+S-LFLIU or EM+M-LFLIU) have significant synergetic bactericidal and anti-biofilm effects and EM+M-LFLIU exhibits superior performance in anti-biofilm. Furthermore, it was suggested that EM+M-LFLIU could produce a large amount of reactive oxygen species (ROS), destroy the integrity of bacterial membrane and wall, down-regulate the expression of oxidative stress, membrane wall synthesis, bacterial virulence and other related genes (agrB, PBP3, sgtB, GMK, zwf, msrA). In vivo study, micro-CT, H&E staining, ELISA assay and bacterial quantification of bone tissue indicated that EM+M-LFLIU could also relieve osteomyelitis of MRSA infection. Our work proffers an original treating bacterial osteomyelitis approach that weakens drug-resistant bacterial and suppresses biofilm formation through SACT, which may provide new prospects for clinical treatment.

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Treatment of MRSA-infected osteomyelitis using bacterial capturing, magnetically targeted composites with microwave-assisted bacterial killing

Cited by 193 publications

References 41 publications

Combating Drug-Resistant Bacteria Using Photothermally Active Nanomaterials: A Perspective Review

Combating Drug-Resistant Bacteria Using Photothermally Active Nanomaterials: A Perspective Review

Stimuli-responsive nanocarriers for bacterial biofilm treatment

Emodin Combined with Multiple Low-frequency Low-intensity Ultrasound to Relieve Osteomyelitis Through Sonoantimicrobial Chemotherapy

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