Treatment of herpes simplex labialis in macule and vesicle phases with photodynamic therapy. Report of two cases

Ramalho, Karen Müller; Rocha, Rodney García; Correa-Aranha, Ana Cecília; Cunha, Sandra Ribeiro; Simões, Alyne; Campos, Luana Moraes; Eduardo, Carlos

doi:10.1016/j.pdpdt.2015.02.005

Cited by 22 publications

(9 citation statements)

References 6 publications

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“…Similarly, Meyers et al [15,16] failed to demonstrate the photodynamic inactivation of recurrent herpetic infection using NR. Recently, Marotti et al [17,18] were successful in the use of PDT as a treatment for herpes labialis, and similar results were obtained by Tardivo et al [19] and Ramalho et al [20] . However, some studies have demonstrated that photoinactivation of HSV-1 and HSV-2 in the presence of NR resulted in transformation of the cells in vitro, and thus PDT may be potentially oncogenic [21][22][23] .…”

Section: Introductionmentioning

confidence: 55%

Transformation of Cells by Photoinactivated Murine Gamma-Herpesvirus 68 during Nonproductive and Quiescent Infection

et al. 2017

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Infection of human MRC-5 cells and mouse NIH-3T3 cells with a murine gamma-herpesvirus (MuHV-4 strain 68; MHV-68) photoinactivated by visible light in the presence of methylene blue (MB) resulted in nonproductive infection and the appearance of morphologically transformed cells. Two stably transformed cell lines were derived from both of these cell types and were confirmed to contain both viral DNA and antigen. Next, a quiescent MHV-68 infection in MRC-5 and NIH-3T3 cells was established after cultivation at 41°C in the presence of phosphonoacetic acid. Following the exposure of quiescently infected cells to visible light for 120 s (5 times daily for 6 days) in the presence of MB, both MRC-5 and NIH-3T3 cells were observed to acquire transformed phenotypes. The cytopathic effect was observed in cells after 4-5 passages, after which the cells degenerated. However, when human interferon (IFN)-α and mouse IFN-β were added to the media of quiescently infected MRC-5 and NIH-3T3 cells during the photoinactivating procedure, 2 stable transformed cell lines containing both viral DNA and the antigen were obtained and resembled those attained following nonproductive infection with photoinactivated virus.

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Section: Introductionmentioning

confidence: 55%

Transformation of Cells by Photoinactivated Murine Gamma-Herpesvirus 68 during Nonproductive and Quiescent Infection

et al. 2017

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“…NR was one of the first, although not very effective, photosensitizers used for treatment of herpetic lesions (Myers et al, 1975(Myers et al, , 1976Roome et al, 1975). However, satisfactory results have been obtained using MB (Chang et al, 1975;Tardivo et al, 2005Tardivo et al, , 2012Marotti et al, 2009Marotti et al, , 2010Sperandio et al, 2009;Ramalho et al, 2015). No transforming ability of any strain of HSV-1 or HSV-2 photoinactivated in the presence of MB has been demonstrated as yet.…”

Section: Introductionmentioning

confidence: 99%

Herpes simplex viruses type 1 and 2 photoinactivated in the presence of methylene blue transform human and mouse cells in vitro

Michútová¹,

Mrázová²,

Kúdelová³

et al. 2017

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Three strains of herpes simplex virus, K17syn- and HSZPsyn+ of type 1 (HSV-1) and USsyn- of type 2 (HSV-2), were photoinactivated in the presence of methylene blue and used to infect 3 cell lines, normal human lung tissue cells (MRC-5), mouse epithelial cells (NIH3T3), and human lung carcinoma cells (A549). The virus titer and phenotype of cells were evaluated to compare the characteristics of normal and carcinoma cells infected with non-syncytial (non-syn) and syncytial (syn) strains of herpes simplex viruses. We found that the cells of both normal cell lines infected with photoinactivated K17syn- and USsyn- but not HSZPsyn+ acquired transformed phenotype accompanied by the presence of virus. Surprisingly, the infection with photoinactivated viruses K17syn- and USsyn- but not HSZPsyn+ resulted in the suppression of the transformed phenotype of A549 cells. Using nested PCR, herpesviral DNA was identified in newly transformed cells and cells that lost the transformed phenotype. The effect of putative herpesvirus-related growth factors (HRGF) produced by cells infected with photoinactivated viruses was quantified and compared. Since methylene blue is currently used in phototherapy of herpetic lesions, these results raise the question of whether such therapy is risky to human health.

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“…Published case reports regarding treatment of recurrent herpes with phototherapy (including laser) using synthetic dyes focus mainly on the effects of reducing the duration of HSV infection symptoms and its accompanying pain, reducing viral titre and acceleration of wound healing, but not on prevention of relapse (3)(4)(5)14). The use of PDT with a relative low dosimetry is indicated in the vesicle phase.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies have shown the effectiveness of PDT in the inactivation of different types of virus in vitro and in vivo (2). The clinical application of PDT in the treatment of recurrent herpes is based mainly on case studies with the use of synthetic dyes as photosensitizers (3)(4)(5). The most common photosensitizer used in PDT is 5-aminolevulinic acid (ALA).…”

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confidence: 99%

Photodynamic Therapy with Red Light and 5-Aminolaevulinic Acid for Herpes Simplex Recurrence: Preliminary Results

Osiecka¹,

Nockowski²,

Kwiatkowski³

et al. 2017

Acta Derm Venerol

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Treatment of herpes simplex labialis in macule and vesicle phases with photodynamic therapy. Report of two cases

Cited by 22 publications

References 6 publications

Transformation of Cells by Photoinactivated Murine Gamma-Herpesvirus 68 during Nonproductive and Quiescent Infection

Transformation of Cells by Photoinactivated Murine Gamma-Herpesvirus 68 during Nonproductive and Quiescent Infection

Herpes simplex viruses type 1 and 2 photoinactivated in the presence of methylene blue transform human and mouse cells in vitro

Photodynamic Therapy with Red Light and 5-Aminolaevulinic Acid for Herpes Simplex Recurrence: Preliminary Results

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