Treatment of hepatocellular carcinoma in a rat model using electrochemotherapy

Possible Involvement of Antitumor Immunity in the Eradication of Colon 26 Induced by Low-Voltage Electrochemotherapy with Bleomycin

“…Furthermore, although the electric fields used in this study were not intense enough to cause measurable tumor destruction, it is possible that enough tumor cells were irreversibly electroporated with the combination treatment of bleomycin to enhance an immune response by releasing antigen. 9 Previous studies have shown that electrochemotherapy with bleomycin successfully cured a higher ratio of transplanted and spontaneously developed tumors in immunocompetent mice than in immunodeficient nude mice. 6,21,22 The induction of cytotoxic T lymphocytes against Colon 26 cells was also observed in the spleens of mice with Colon 26 after treatment with high-voltage electrochemotherapy and bleomycin.…”

“…4,5 Electrochemotherapy has been found to increase immune response to tumors as well [6][7][8] and the infiltration of inflammatory cells into tumor nodules treated with bleomycin and electric pulse was also reported. 9 Based on this phenomenon, it is possible that a host-immune mechanism is involved in tumor regression after electrochemotherapy; however, its precise nature has yet to be defined. Furthermore, while a lower level of electric power is desirable in a clinical setting, few studies have reported success with using low-voltage electroporation to inhibit tumor growth.…”

Electroporation-Enhanced Nonviral Gene Transfer for the Prevention or Treatment of Immunological, Endocrine and Neoplastic Diseases

“…EP is traditionally performed in conjunction with chemotherapy for different malignancies. This treatment, electrochemotherapy, has been successful for liver malignancies in animal models [Jaroszeski, M.J. et al, 2001]. Recently, tumor reduction has been achieved by locally injecting DNA to the site of interest in the liver followed by the electric field application [Gilbert, R. et al, 2002;Harada, N. et al, 2004].…”

Radiosensitizing Effect of Electrochemotherapy in a Fractionated Radiation Regimen in Radiosensitive Murine Sarcoma and Radioresistant Adenocarcinoma Tumor Model

“…Electrochemotherapy is used in the treatment of recurrent cutaneous and subcutaneous tumor nodules of different histology, predominantly in palliative care (10,(14)(15)(16)(17)(18)35). In both preclinical and clinical studies, all tumor types have responded positively to the treatment (9,10,12,(14)(15)(16)(17)(18)(35)(36)(37)(38)(39). However, some preclinical studies have demonstrated a variable response to the treatment that was due to differences in intrinsic tumor cell responsiveness to the drug used (cisplatin or bleomycin) in conjunction with electric pulses (9,39,40).…”