Treatment of experimental allergic encephalomyelitis (EAE) induced by guinea pig myelin basic protein epitope 72–85 with a Human MBP 87–99 analogue and effects of cyclic peptides

Modulatory effect of celastrol on Th1/Th2 cytokines profile, TLR2 and CD3+ T-lymphocyte expression in a relapsing–remitting model of multiple sclerosis in rats

“…As reported previously, the re-challenge by a booster dose of MBP exhibited higher NO levels than immunization with a primary dose only (O'Brien et al, 1999) that seems to render the model more vulnerable to the inhibitory effect of aminoguanidine and induction of relapse. In regard that multiple sclerosis is basically an autoimmune disease that proposed to be mediated by T-lymphocytes that recognize myelin components of the CNS (Hafler and Weiner, 1989;Bernard and Kerlero de Rosbo, 1992), EAE is considered the suitable model to assess the new ways to suppress the demyelinating diseases especially MS (Tselios et al, 2000). In this context, the EAE model in this work exhibited a T cell-mediated immune response in favor to Th1 pattern over Th2 pattern reflected as an imbalance in their associated cytokines where the pro-inflammatory TNF-α was significantly increased without change in the anti-inflammatory IL-10.…”

A putative bioactive conformation for the altered peptide ligand of myelin basic protein and inhibitor of experimental autoimmune encephalomyelitis [Arg91, Ala96] MBP87–99

“…Because linear peptides are a good starting point for intensive conformational studies and rational drug design, this work has been focused on an investigation of the 3D structure of such an APL, a linear EAE antagonist [Arg 91 , Ala 96 ] MBP 87-99 [6,18] (Fig. 1) in which the amino acids Lys 91 and Pro 96 were replaced by Arg and Ala, respectively.…”

Conformational studies of immunodominant myelin basic protein 1–11 analogues using NMR and molecular modeling

“…However, Gln 3 (TCR contact) was not solvent exposed so as to interact with TCR for the antagonist MBP 1-8 [4A] peptide. Knowledge of the relationship of peptide conformation and bioactivity will facilitate the rational design and synthesis The linear peptides were prepared on 2-chlorotrityl chloride resin (CLTR-Cl) using the Fmoc/tBu solid-phase peptide synthetic method [31][32][33][34][35]. The first N a Fmoc (9-fluorenylmethyloxycarboxyl)-protected amino acid Fmoc-Gly-OH was coupled (esterified) to the resin in the presence of diisopropylethylamine (DIPEA) (4.5 equiv, 0.75 mL) in dichloromethane (DCM) in 1 h at RT.…”