Treatment of Equine Allergic Diseases with Allergy Neutralization. A Field Study

Mansfield, James R.

doi:10.1080/13590849861907

Cited by 8 publications

(4 citation statements)

References 11 publications

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“…We suggest that the improvement of knowledge in the pathogenesis of CHS could also favour the development of better diagnostic methods and new therapy protocols. As successful desensitization has been reported by some authors (Mansfield et al., 1998), the production of a recombinant antigen involved in triggering CHS could allow the development of a desensitizing protocol.…”

Section: Introductionmentioning

confidence: 85%

Equine Culicoides Hypersensitivity: Evaluation of a Skin Test and of Humoral Response

Ferroglio

Pregel

Accossato

et al. 2006

Journal of Veterinary Medicine Series A

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Intradermal tests were carried out on 18 horses with clinical signs of Culicoides hypersensitivity (CHS) and 23 horses without clinical signs of CHS, and sera from these horses were analysed by SDS-PAGE and Western blotting (W-B). Intradermal injections of 0.1 ml of 25 microg/microl sterile Culicoides extract, 0.1 ml of 1:10,000 histamine (positive control) and 0.1 ml of physiological saline (negative control) were made in the dermis of the middle region of the neck. Analysis of reactions indicated that a 1 cm wheal and a skinfold thickness >10% at 24 h represented a valid cut-off between horses with and without CHS. In these conditions the test, even in winter when clinical signs were absent, had 100% sensitivity and specificity. The W-B was performed after running Culicoides extract on a 12% polyacrylamide gel. The test revealed the presence of several bands with molecular weight ranging from 6 to 200 kDa. In particular, a band of 65 kDa was predominantly found in hypersensitive horses by using an anti-IgE antibody while in normal horses the same band was mainly detected by using an anti-IgG antibody. Our results demonstrated that the skin test is a valid diagnostic test, with high sensitivity and specificity and that the band of about 65 kDa probably corresponds to the allergen involved in the pathogenesis of CHS.

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Section: Introductionmentioning

confidence: 85%

Equine Culicoides Hypersensitivity: Evaluation of a Skin Test and of Humoral Response

Ferroglio

Pregel

Accossato

et al. 2006

Journal of Veterinary Medicine Series A

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“…In various other diseases of the horse an IgE‐mediated aetiology has been suspected but this remains controversial. These diseases include inflammatory airway disease (IAD), also known as recurrent airway obstruction (RAO, chronic obstructive pulmonary disease (COPD) or heaves ), and head‐shaking . Inflammatory airway disease can be induced by a hyperreactive response of the lower respiratory tract to inhaled moulds and organic dusts .…”

Section: Ige‐mediated Allergies In Horsesmentioning

confidence: 99%

Immunoglobulin E and allergy

Wagner

2015

Equine Veterinary Journal

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In this issue of Equine Veterinary Journal, Dupont and co-workers report on a study to evaluate the consistency of the results obtained with a commercially available immunoglobulin E (IgE)-based test for food allergy diagnosis in ponies with a provocation trial with the presumed allergens [1]. Their results suggest the IgE-based test is not a reliable screening tool for food allergy in healthy equids.

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“…In horses, type I hypersensitivity has been investigated, suggesting that this reaction might be involved in immune-mediated diseases such as summer eczema (Larsen et al 1988;Ungar-Waron et al 1990), urticaria, and head shaking (Mansfield et al 1998), as well as RAO (Halliwell et al 1993;Schmallenbach et al 1998;Eder et al 2000;Eder et al 2001;Curik et al 2003;Künzle et al 2007; Morán et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Detection of reaginic antibodies against Faenia rectivirgula from the serum of horses affected with Recurrent Airway Obstruction by an in vitro bioassay

et al. 2010

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Reaginic antibodies, mainly of the IgE and some IgG subclasses, play an important role in the induction of type I immediate hypersensitivity reactions. These antibodies bind through their Fc fragment to high affinity receptors (FcεRI) present in the membrane of mast cells and basophils. Previously, several studies have investigated the role of reaginic antibodies in the pathogenesis of RAO. However, whereas immunological aspects of RAO have been extensively studied, the precise sequence of events is still not well understood and role of IgE in this disease still remains controversial. Therefore, in this study a bioassay was developed for reaginic antibody determination in serum from RAO-affected horses in order to determine the etiology of disease. The technique involves measuring in vitro calcium mobilization in RBL-2H3 cells following incubation with horse serum from RAO-affected or unaffected horses and one of the RAO antigens (Faenia rectivirgula). Results demonstrated that 15% of samples from the RAO-affected horses reacted positively in this in vitro bioassay, whereas the samples from unaffected horses did not. This bioassay indicates that reaginic antibodies could be involved in the immunological mechanism leading to RAO; and this technique may facilitate future research in other allergic diseases in horses.

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Treatment of Equine Allergic Diseases with Allergy Neutralization. A Field Study

Cited by 8 publications

References 11 publications

Equine Culicoides Hypersensitivity: Evaluation of a Skin Test and of Humoral Response

Equine Culicoides Hypersensitivity: Evaluation of a Skin Test and of Humoral Response

Immunoglobulin E and allergy

Detection of reaginic antibodies against Faenia rectivirgula from the serum of horses affected with Recurrent Airway Obstruction by an in vitro bioassay

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