Treatment of Epidermal Growth Factor (EGF) enhances Nuclear Maturation of Porcine Oocytes and Stimulates Expression of ER/Golgi Transport Proteins

Hwangbo, Yong; Oh, Hae-In; Lee, Sang‐Hee; Cheong, Hyeonsook; Yang, Boo‐Keun; Park, Choon‐Keun

doi:10.12717/dr.2017.21.2.131

Cited by 11 publications

(8 citation statements)

References 30 publications

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“…2f) supports the link between Z-disc and sarcoplasmic reticulum (SR), where Speg controls calcium reuptake in diastole 22 , a process in tune with titin's elastic properties that determine diastolic filling. Spectrins have been linked to disrupted Z-discs 23 and Copg2 is involved in ER-Golgi transport 24 . In the majority of these proteins, biotinylation is limited to a single site outside a functional domain and replicated in the heart and skeletal muscle ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Deconstructing sarcomeric structure–function relations in titin-BioID knock-in mice

et al. 2020

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Proximity proteomics has greatly advanced the analysis of native protein complexes and subcellular structures in culture, but has not been amenable to study development and disease in vivo. Here, we have generated a knock-in mouse with the biotin ligase (BioID) inserted at titin's Z-disc region to identify protein networks that connect the sarcomere to signal transduction and metabolism. Our census of the sarcomeric proteome from neonatal to adult heart and quadriceps reveals how perinatal signaling, protein homeostasis and the shift to adult energy metabolism shape the properties of striated muscle cells. Mapping biotinylation sites to sarcomere structures refines our understanding of myofilament dynamics and supports the hypothesis that myosin filaments penetrate Z-discs to dampen contraction. Extending this proof of concept study to BioID fusion proteins generated with Crispr/CAS9 in animal models recapitulating human pathology will facilitate the future analysis of molecular machines and signaling hubs in physiological, pharmacological, and disease context.

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Section: Resultsmentioning

confidence: 99%

Deconstructing sarcomeric structure–function relations in titin-BioID knock-in mice

et al. 2020

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“…It was found that the percentage of cleavage increased from 70 to 90% (approximately 28%) in the presence of the EGF in the group-IVM culture. EGF has been reported to mediate oocyte maturation by phosphorylation of mitogen-activated protein kinase (MAPK) 34,35 . In fact, the suppression of EGFR signaling abolishes the acquisition of oocyte developmental competence 34,35 .…”

Section: Discussionmentioning

confidence: 99%

“…EGF has been reported to mediate oocyte maturation by phosphorylation of mitogen-activated protein kinase (MAPK) 34 , 35 . In fact, the suppression of EGFR signaling abolishes the acquisition of oocyte developmental competence 34 , 35 . Previous studies have reported that the MAPK pathway and the EGFR signaling are inhibited by urea and its derivatives 9 – 11 , 36 .…”

Section: Discussionmentioning

confidence: 99%

A multilevel analysis identifies the different relationships between amino acids and the competence of oocytes matured individually or in groups

Kowsar

Mansouri

Sadeghi

et al. 2020

Sci Rep

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High-protein diets contribute to an increase in urea follicular concentrations associated with decreased fertility. Urea has been shown to interfere with the epidermal growth factor (EGF)/EGFR system, which has been shown to have a beneficial effect during in vitro maturation (IVM) of oocytes. Of note, the number of cumulus-oocyte complexes (COCs) in the maturation medium can change the maturation and the developmental competence of COCs. Therefore, it was hypothesized that, the presence of urea and EGF may have a differential effect on the depletion/appearance of AAs and competence of COCs matured individually (I-IVM system) or in groups (G-IVM system). In the G-IVM system, COCs increased consumption (depletion) of AAs compared with other groups in the presence of high-level urea (40 mg/dl) + EGF (10 ng/ml). In the I-IVM system, the non-cleaved COCs depleted more AAs than the cleaved COCs, in particular in the presence of urea. The combination of urea and EGF increased the depletion of AAs in the G-IVM system. However, the EGF abrogated the urea-induced depletion of AAs by the I-IVM COCs. The use of N-acetyl-l-cysteine as an EGFR inhibitor canceled urea-induced depletion of AAs. This shows the inhibiting effect of urea over the EGF/EGFR system. In the presence of urea + EGF, COCs had a lower degree of developmental competence than control in both I- and G-IVM systems. Arginine had the best predictive power to identify highly competent COCs in the G-IVM system, while glutamine was the best predictor of the cleavage in the I-IVM system. In conclusion, this multi-level study shows that COCs matured individually or in groups may have different association with AAs metabolism. These findings provide new insights into the relationships between AA metabolism and the subsequent developmental competence of COCs.

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“…Additionally, melatonin can ameliorate decreased embryo development caused by the AKT1 inhibitor SH6 during the in vitro maturation step by enhancing oocyte maturation, cumulus cell expansion, and protection from DNA fragmentation (56). EGFR also plays an essential role in ovarian function (57)(58)(59). LH-induced EGFR activation is an essential component for the communication between the outer mural granulosa and theca cells and the inner cumulus cells and oocytes, leading to cumulus cell expansion and oocyte maturation (60).…”

Section: Melatonin's Eight Hub Targets In Dormentioning

confidence: 99%

Melatonin: Multi-Target Mechanism Against Diminished Ovarian Reserve Based on Network Pharmacology

Yang

Chen

et al. 2021

Front. Endocrinol.

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BackgroundDiminished ovarian reserve (DOR) significantly increases the risk of female infertility and contributes to reproductive technology failure. Recently, the role of melatonin in improving ovarian reserve (OR) has attracted widespread attention. However, details on the pharmacological targets and mechanisms of melatonin-improved OR remain unclear.ObjectiveA systems pharmacology strategy was proposed to elucidate the potential therapeutic mechanism of melatonin on DOR at the molecular, pathway, and network levels.MethodsThe systems pharmacological approach consisted of target identification, data integration, network construction, bioinformatics analysis, and molecular docking.ResultsFrom the molecular perspective, 26 potential therapeutic targets were identified. They participate in biological processes related to DOR development, such as reproductive structure development, epithelial cell proliferation, extrinsic apoptotic signaling pathway, PI3K signaling, among others. Eight hub targets (MAPK1, AKT1, EGFR, HRAS, SRC, ESR1, AR, and ALB) were identified. From the pathway level, 17 significant pathways, including the PI3K-Akt signaling pathway and the estrogen signaling pathway, were identified. In addition, the 17 signaling pathways interacted with the 26 potential therapeutic targets to form 4 functional modules. From the network point of view, by regulating five target subnetworks (aging, cell growth and death, development and regeneration, endocrine and immune systems), melatonin could exhibit anti-aging, anti-apoptosis, endocrine, and immune system regulation effects. The molecular docking results showed that melatonin bound well to all hub targets.ConclusionThis study systematically and intuitively illustrated the possible pharmacological mechanisms of OR improvement by melatonin through anti-aging, anti-apoptosis, endocrine, and immune system regulation effects.

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Treatment of Epidermal Growth Factor (EGF) enhances Nuclear Maturation of Porcine Oocytes and Stimulates Expression of ER/Golgi Transport Proteins

Cited by 11 publications

References 30 publications

Deconstructing sarcomeric structure–function relations in titin-BioID knock-in mice

Deconstructing sarcomeric structure–function relations in titin-BioID knock-in mice

A multilevel analysis identifies the different relationships between amino acids and the competence of oocytes matured individually or in groups

Melatonin: Multi-Target Mechanism Against Diminished Ovarian Reserve Based on Network Pharmacology

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