Treatment of dehiscence‐type defects with collagen matrix and/or enamel matrix derivative: Histomorphometric study in minipigs

Abstract

BackgroundThis study aimed to evaluate, histomorphometrically, the use of collagen matrix (CM) and/or enamel matrix derivative (EMD) for the treatment of dehiscence‐type recession defects in minipigs.MethodsEight healthy, male, young BR‐1 minipigs, with no periodontal disease were treated. Bilateral dehiscence‐type defects were surgically created on the buccal of the mandibular premolars (PI and PII). After 30 days, the defects were randomly assigned to four groups: coronally advanced flap (CAF); CAF + CM; CAF + EMD; and CAF + CM + EMD (split‐mouth design). The evaluated parameters (mm): total defect length; new cementum (NC); new bone (NB); gingival margin position; total epithelium length; epithelium on the root; connective tissue adaptation; and soft tissue thickness (STT).ResultsThe EMD‐treated groups showed a superior length of NC [4.13 ± 1.22 (CAF + EMD); 3.95 ± 1.11 (CAF + CM + EMD); 2.94 ± 0.77 (CAF + CM); 2.72 ± 0.81 (CAF), P = 0.02] and NB [3.21 ± 0.68 (CAF + CM + EMD); 3.01 ± 0.56 (CAF + EMD); 2.15 ± 0.47 (CAF + CM); 2.29 ± 0.82 (CAF), P = 0.005]. The CAF and CAF + CM groups showed a superior epithelial length when compared to EMD‐treated groups after 3 months. A superior STT was observed for CAF + CM + EMD group (1.5 ± 0.33) when compared with the other groups [1.09 ± 0.26 (CAF + EMD); 1.04 ± 0.34 (CAF + CM); and 1.14 ± 0.29 (CAF), P = 0.03].Conclusion(s)The results of the present study indicate that EMD application, irrespective of the combination with CM, may improve the periodontal regeneration of dehiscence‐type defects in this animal model.

Comparison of the effectiveness of Ankaferd Blood Stopper® and Emdogain in periodontal regeneration

“…The early and long-term healing results in the present study were similar to those of França-Grohmann et al ( 2020), who reported that the use of EMD in periodontal regeneration increased the new cementum and new bone formation in an experimental study in minipigs (França-Grohmann et al, 2020). Other experimental studies reported similar results regarding the effectiveness of EMD for periodontal regeneration (Casati et al, 2002;Sallum et al, 2004;Takeda et al, 2018).…”

Comparison of two types of xenogeneic matrices to treat single gingival recessions: A randomized clinical trial

“…16,19,20 In addition, because the matrices have different manufacturing processes that lead to different structural and physical characteristics, they may have different outcomes. 22,23 Thus, the aim of the present study was to evaluate the superiority of clinical and patient-centered outcomes of two types of XM (both associated with the CAF technique) versus CAF alone to treat single gingival recession, as well as to make direct comparisons and evaluate whether one of the matrices is superior to the other.…”

Effectiveness of Adjunctive Hyaluronic Acid Application in Surgical Treatment of Gingival Recession Sites

“…In addition, the use of an enamel matrix derivative (EMD) has been proposed to improve the effectiveness of soft tissue coverage of exposed root surfaces [5]. Studies showed that CAF combined with sCTG or EMD increases the probability of achieving complete root coverage (CRC) in Miller class I and II [6] and Cairo type I [7] recession defects compared to CAF alone [8][9][10]. The satisfying results achieved with the use of EMD have encouraged clinicians to introduce another organic molecule in periodontal therapy, namely hyaluronic acid (HA).…”