Treatment of corneal chemical alkali burns with a crosslinked thiolated hyaluronic acid film

Abstract

CMHA-S films facilitate re-epithelialization and decrease the area of corneal opacity in our corneal alkali burn rabbit model.

Anti-Inflammatory and Anti-Fibrotic Effects of Human Amniotic Membrane Mesenchymal Stem Cells and Their Potential in Corneal Repair

“…All procedures were performed under deep anesthesia and all available efforts were made to prevent any pain. Six animals per group were randomly assigned as follows : group 1 was used as a control group and was administered no corneal alkali‐burn lesion nor hAM‐MSC treatment (control group); group 2 were NaOH‐injured and treated only with 300 μL of BSS which was injected into the anterior chamber of the eye (NaOH group); the rabbits in group 3 were injured with NaOH, following which 1 × 10 6 hAM‐MSC in 300 μL of balanced salt solution (BSS) was injected into the anterior chamber of the eye (NaOH‐hAM‐MSC group). All the injections were performed using a 1 mL, 27‐gauge syringe (BD, Mexico City, Mexico).…”

Dynamic changes of the extracellular matrix during corneal wound healing

“…A wide variety of corneal alkali burn models in rabbit have been described. Most studied the wound healing process and the effect of different treatments only in the acute and early reparative phases (Griffith GL et al, 2018;Rezaei Kanavi et al, 2016). Some studies included the late reparative phase (Huang et al, 2001;Kubota M, 1991), but they did not take a multidisciplinary approach in the examination of changes in the ECM.…”

“…Several animal models of alkali burn have been used to evaluate the corneal wound healing process (Choi et al, 2017) and to test drugs that reduce the scarring or corneal opacity (Griffith GL et al, 2018;Haddox et al, 2001;Lopes et al, 2017;Zahir-Jouzdani F, 2018). All of the procedures use sodium hydroxide to produce the alkali burn.…”

Amniotic membrane allografts maintain key biological properties post SCCO2 and lyophilization processing

“…The left eye of each rabbit was topically anesthetized with 0.5% tetracaine hydrochloride and disinfected with 5% betadine (Alcon, Fort Worth, TX) prior to the surgical removal of the nictitating membrane, as previously described (Figure 2(a) and (b)). 13 Post-nictitating membrane removal surgery, the eyes were treated with Bacitracin ophthalmic ointment (Figure 2(c)) and allowed to heal for two weeks prior to the placement of the amniotic membrane.…”