Treatment of Central Venous Catheter Fungal Infection Using Liposomal Amphotericin-B Lock Therapy

Buckler, Bradley S.; Sams, Ralph N.; Goei, Vita Lan-Ing; Krishnan, Karthik R.; Bemis, Marci J.; Parker, Deidrea P.; Murray, Dennis L.

doi:10.1097/inf.0b013e318170b68b

Cited by 50 publications

(30 citation statements)

References 8 publications

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“…It should be noted that the miconazole concentrations used in our in vitro biofilm setup (0 to 2.4 mM) to observe the phenomenon of miconazole-tolerant persisters are higher than the commonly used therapeutic miconazole concentrations. However, such high concentrations are achievable during antifungal lock therapy (2,16). In the present study, we demonstrated an important role for superoxide dismutases in the occurrence of miconazole-tolerant persisters in C. albicans biofilms by using the Cu,Zn-Sod inhibitor N,NЈ-diethyldithiocarbamate (DDC).…”

Section: Discussionmentioning

confidence: 84%

Superoxide Dismutases Are Involved in Candida albicans Biofilm Persistence against Miconazole

Bink

Vandenbosch

Coenye

et al. 2011

Antimicrob Agents Chemother

109

104

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We investigated the cellular mechanisms responsible for the occurrence of miconazole-tolerant persisters in Candida albicans biofilms. Miconazole induced about 30% killing of sessile C. albicans cells at 75 M. The fraction of miconazole-tolerant persisters, i.e., cells that can survive high doses of miconazole (0.6 to 2.4 mM), in these biofilms was 1 to 2%. Since miconazole induces reactive oxygen species (ROS) in sessile C. albicans cells, we focused on a role for superoxide dismutases (Sods) in persistence and found the expression of Sod-encoding genes in sessile C. albicans cells induced by miconazole compared to the expression levels in untreated sessile C. albicans cells. Moreover, addition of the superoxide dismutase inhibitor N,N-diethyldithiocarbamate (DDC) to C. albicans biofilms resulted in an 18-fold reduction of the miconazole-tolerant persister fraction and in increased endogenous ROS levels in these cells. Treatment of biofilms of C. albicans clinical isolates with DDC resulted in an 18-fold to more than 200-fold reduction of their miconazole-tolerant persister fraction. To further confirm the important role for Sods in C. albicans biofilm persistence, we used a ⌬sod4 ⌬sod5 mutant lacking Sods 4 and 5. Biofilms of the ⌬sod4 ⌬sod5 mutant contained at least 3-fold less of the miconazole-tolerant persisters and had increased ROS levels compared to biofilms of the isogenic wild type (WT). In conclusion, the occurrence of miconazole-tolerant persisters in C. albicans biofilms is linked to the ROS-detoxifying activity of Sods. Moreover, Sod inhibitors can be used to potentiate the activity of miconazole against C. albicans biofilms.

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Section: Discussionmentioning

confidence: 84%

Superoxide Dismutases Are Involved in Candida albicans Biofilm Persistence against Miconazole

Bink

Vandenbosch

Coenye

et al. 2011

Antimicrob Agents Chemother

109

104

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“…Most studies (5/7) reported 70 called for an RCT of CVC removal compared with retention in patients with candidaemia to address these serious biases in observational studies. 71 We included seven retrospective cohort studies that reported the association between CVC removal and risk of death, complications or recurrent infection in children (see Table 11). [45][46][47][48][49][50][51] The studies involved different patient groups (four studies were confined to neonates) [47][48][49][50] and different types of bloodstream infection (five studies were restricted to specific organisms), 45,[47][48][49]51 making it difficult to assess consistency of results.…”

Section: Resultsmentioning

confidence: 99%

Accuracy of bacterial DNA testing for central venous catheter-associated bloodstream infection in children with cancer

Millar

Zhou²,

Skinner³

et al. 2011

Health Technol Assess

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How to obtain copies of this and other HTA programme reports An electronic version of this title, in Adobe Acrobat format, is available for downloading free of charge for personal use from the HTA website (www.hta.ac.uk). A fully searchable DVD is also available (see below).Printed copies of HTA journal series issues cost £20 each (post and packing free in the UK) to both public and private sector purchasers from our despatch agents.Non-UK purchasers will have to pay a small fee for post and packing. For European countries the cost is £2 per issue and for the rest of the world £3 per issue. How to order:-fax (with credit card details) -post (with credit card details or cheque) -phone during office hours (credit card only).Additionally the HTA website allows you to either print out your order or download a blank order form. Contact details are as follows:Synergie UK (HTA Department) Digital House, The Loddon Centre Wade Road Basingstoke Hants RG24 8QW Email: orders@hta.ac.uk Tel: 0845 812 4000 -ask for 'HTA Payment Services' (out-of-hours answer-phone service) Fax: 0845 812 4001 -put 'HTA Order' on the fax header Payment methods Paying by chequeIf you pay by cheque, the cheque must be in pounds sterling, made payable to University of Southampton and drawn on a bank with a UK address.Paying by credit card You can order using your credit card by phone, fax or post. SubscriptionsNHS libraries can subscribe free of charge. Public libraries can subscribe at a reduced cost of £100 for each volume (normally comprising 40-50 titles). The commercial subscription rate is £400 per volume (addresses within the UK) and £600 per volume (addresses outside the UK). Please see our website for details. Subscriptions can be purchased only for the current or forthcoming volume.How do I get a copy of HTA on DVD?Please use the form on the HTA website (www.hta.ac.uk/htacd/index.shtml). HTA on DVD is currently free of charge worldwide.The website also provides information about the HTA programme and lists the membership of the various committees. ii ii HTA NIHR Health Technology Assessment programmeThe Health Technology Assessment (HTA) programme, part of the National Institute for Health Research (NIHR), was set up in 1993. It produces high-quality research information on the effectiveness, costs and broader impact of health technologies for those who use, manage and provide care in the NHS. 'Health technologies' are broadly defined as all interventions used to promote health, prevent and treat disease, and improve rehabilitation and long-term care. The research findings from the HTA programme directly influence decision-making bodies such as the National Institute for Health and Clinical Excellence (NICE) and the National Screening Committee (NSC). HTA findings also help to improve the quality of clinical practice in the NHS indirectly in that they form a key component of the 'National Knowledge Service' . The HTA programme is needs led in that it fills gaps in the evidence needed by the NHS. There are three routes to the start of pro...

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“…Dans les études de Ko, Oncu et Shuford [26,27,29] [21] ont ainsi traité des infections sur cathéter à C. albicans [21], C. glabrata [21,31], C. parapsilosis [20,21] ou C. guillermondii [21], par des verrous utilisant l'amphotéricine B (déoxycholate ou liposomale) à des concentrations comprises entre 2,7 et 5 g/l en association à un traitement systémique par le fluconazole [31] ou l'amphotéricine B [20,21]. Selon Schinabeck et al [28], l'intérêt des traitements verrous est de lutter contre la résistance croissante des microorganismes se développant au sein d'un biofilm, d'éviter la toxicité des molécules utilisées dans le traitement systémique et ainsi de s'affranchir des dosages plasmatiques de ces molécules et, finalement, d'éviter la dissémination des microorganismes en verrouillant le cathéter.…”

Section: Verrous Fongiques : Modèles Et Efficacitéunclassified

Candidoses associées aux cathéters

Cateau¹,

Rodier²,

Imbert³

2012

Med Sci (Paris)

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Treatment of Central Venous Catheter Fungal Infection Using Liposomal Amphotericin-B Lock Therapy

Cited by 50 publications

References 8 publications

Superoxide Dismutases Are Involved in Candida albicans Biofilm Persistence against Miconazole

Superoxide Dismutases Are Involved in Candida albicans Biofilm Persistence against Miconazole

Accuracy of bacterial DNA testing for central venous catheter-associated bloodstream infection in children with cancer

Candidoses associées aux cathéters

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