Treatment of Brucellosis in Guinea Pigs via a Combination of Engineered Novel pH-Responsive Curcumin Niosome Hydrogel and Doxycycline-Loaded Chitosan–Sodium Alginate Nanoparticles: an In Vitro and In Vivo Study

El-Ela, Fatma I. Abo; Hussein, Khaled; El-Banna, H. A.; Gamal, Amr; Rouby, Sherin; Menshawy, Ahmed M. S.; El‐Nahass, El‐Shaymaa; Anwar, Shehata; Zeinhom, Mohamed M.A.; Salem, Heba F.; Al-Sayed, Marawa Ahmed Yahia; El-Newery, Hala A.; Shokier, Khaled A. M.; El-Nesr, Khalid A.; Hosein, H. I.

doi:10.1208/s12249-020-01833-7

Cited by 17 publications

(9 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, the size was significantly (p < 0.05) increased by increasing X 2, where F3 had a vesicle size of 295.33 ± 2.60 nm with a PDI of 0.263 ± 0.05 and F13 had a vesicle size of 122.13 ± 2.63 nm with a PDI of 0.194 ± 0.01. The zeta potential method is employed in order to determine the surface charge of nanoparticles [40]. The colloidal stability of the produced formulations can be predicted by the zeta potential.…”

Section: Zeta Potential and Particle Size Determinationmentioning

confidence: 99%

The Impact of Improving Dermal Permeation on the Efficacy and Targeting of Liposome Nanoparticles as a Potential Treatment for Breast Cancer

et al. 2021

Self Cite

View full text Add to dashboard Cite

Breast cancer is the most frequent malignancy in women. This work focuses on developing deformable liposomes as a potential carrier for breast cancer treatment and studying the impact of improving dermal permeation on the efficacy and targeting of liposomes. Raloxifene (RXF), an oestrogen antagonist, was used as a model drug. Using Box–Behnken design, different formulations of RXF-loaded deformable liposome (RLDL) were prepared using different propylene glycol, phospholipid and cholesterol concentrations. The percentage of entrapment efficiency (Y1), particle size (Y2), zeta potential (Y3) and steady-state flux (Y4) of the prepared formulations were all evaluated. Y1 and Y4 were significantly increased and Y2 and Y3 were significantly decreased when the propylene glycol concentration was increased. The optimization was obtained and the optimum formulation was that including phospholipid (1.40% w/w), cholesterol (0.15% w/w) and propylene glycol (10% v/v). The selected optimum formulation displayed a % EE of 78.34 ± 1.04% with a steady-state flux of 4.21 ± 0.02 µg/cm2/h. In order to investigate bioavailability, antitumor effectiveness and permeation, the optimum formulation was selected and included in a carbopol gel. The optimum gel formulation had 2.77 times higher bioavailability and, as a result, considerable antitumor action as compared to oral RXF. In conclusion, optimum RLDL gel may be an effective breast cancer treatment.

show abstract

Section: Zeta Potential and Particle Size Determinationmentioning

confidence: 99%

The Impact of Improving Dermal Permeation on the Efficacy and Targeting of Liposome Nanoparticles as a Potential Treatment for Breast Cancer

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Methotrexate content was determined by cooling centrifuging (SIGMA, Steinheim, Germany). A sample of the prepared MTX-GNPs formulations were centrifuged at 20,000 rpm for 1 h [ 45 ]. The supernatant solution was collected and the %EE was calculated in triplicates using the UV-VIS spectrophotometer method at the maximum wavelength of 303 nm as follows: %EE = (B − A)/B × 100 where A is the drug amount in supernatant, and B is the total drug amount.…”

Section: Discussionmentioning

confidence: 99%

Nano Methotrexate versus Methotrexate in Targeting Rheumatoid Arthritis

et al. 2022

View full text Add to dashboard Cite

Nanomedicine has emerged as an important approach for targeting RA medication. Rheumatoid arthritis (RA) is a widespread autoimmune disorder marked by multiple inflamed joints. Gold nanoparticles (GNPs) have been demonstrated as efficacious nanocarriers due to their unique characteristics and the relative simplicity of their synthesis in varied sizes; moreover, they have the capability to alleviate several inflammatory markers. The current objective was to combine methotrexate (MTX) with GNPs to overcome MTX restrictions. GNPs were fabricated by a chemical reduction technique, utilizing sodium citrate and tween 20. The MTX-GNPs formulations were characterized in vitro by % entrapment efficiency (%EE), particle size, polydispersity index (PDI) zeta potential, and % release. The MTX-GNPs formulation was administrated as an intra-articular solution, and additionally, incorporated into a Carbopol gel to investigate its anti-arthritic effectiveness and bioavailability in vivo. The results indicated that a %EE of 87.53 ± 1.10%, and a particle size of 60.62 ± 2.41 nm with a PDI of 0.31 ± 0.03, and a zeta potential of −27.80 ± 0.36 mV were optimal. The in vitro release of MTX from the MTX-GNPs formulation demonstrated that the MTX-GNPs formulation’s release was 34.91± 1.96% and considerably (p < 0.05) lower than that of free MTX, showing a significant difference in dissolution patterns (p < 0.05). In vivo, MTX-GNPs formulations inhibited IL-6 by 36.52%, ACCP (63.25 %), COMP (28.16%), and RANKL (63.67%), as well as elevated IL-10 by 190.18%. Transdermal MTX-GNPs decreased IL-6 by 22.52%, ACCP (56.63%), COMP (52.64%), and RANKL (79.5%), as well as increased IL-10 by 168.37%. Histological investigation supported these recent findings. Conclusions: Marked improvements in MTX anti-arthritic effects are seen when it is conjugated to GNPs.

show abstract

“…To assess the EUC content, the EUC-NE formulation sample was centrifuged (SIGMA, Steinheim, Germany) for 1 h at 20,000 rpm ( Abo El-Ela et al, 2020 ). The supernatant solution was collected and the %EE was calculated in triplicates using the GC-MS method as follows:

…”

Section: Methodsmentioning

confidence: 99%

In vitro evaluation of nebulized eucalyptol nano-emulsion formulation as a potential COVID-19 treatment

Tulbah

Bader

Ong

et al. 2022

Saudi Pharmaceutical Journal

View full text Add to dashboard Cite

Treatment of Brucellosis in Guinea Pigs via a Combination of Engineered Novel pH-Responsive Curcumin Niosome Hydrogel and Doxycycline-Loaded Chitosan–Sodium Alginate Nanoparticles: an In Vitro and In Vivo Study

Cited by 17 publications

References 42 publications

The Impact of Improving Dermal Permeation on the Efficacy and Targeting of Liposome Nanoparticles as a Potential Treatment for Breast Cancer

The Impact of Improving Dermal Permeation on the Efficacy and Targeting of Liposome Nanoparticles as a Potential Treatment for Breast Cancer

Nano Methotrexate versus Methotrexate in Targeting Rheumatoid Arthritis

In vitro evaluation of nebulized eucalyptol nano-emulsion formulation as a potential COVID-19 treatment

Contact Info

Product

Resources

About