Treatment of BG-1 Ovarian Cancer Cells Expressing Estrogen Receptors with Lambda-cyhalothrin and Cypermethrin Caused a Partial Estrogenicity Via an Estrogen Receptor-dependent Pathway

Kim, Cho‐Won; Go, Ryeo‐Eun; Choi, Kyung‐Chul

doi:10.5487/tr.2015.31.4.331

Cited by 16 publications

(11 citation statements)

References 26 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To meet the market demand for quality horticultural crops, farmers use various pesticides (Table 2). [34][35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52][53] Lekei et al 54 identified a total of 1182 pesticide products registered in Tanzania, representing a broad variety of active ingredients. Arusha region is known to be leading in pesticide trading and utilization in Tanzania because of intensification of horticulture.…”

Section: Pesticide Use In Horticulturementioning

confidence: 99%

Pesticide Exposure and Health Problems Among Female Horticulture Workers in Tanzania

Mrema

Ngowi

Kishinhi

et al. 2017

Environ�Health�Insights

View full text Add to dashboard Cite

Commercialization of horticulture farming, expansion of farms, and the practice of monoculture favor the proliferation of pests, which in turn increases the need for pesticides. Increased exposure to pesticides is associated with inadequate knowledge on the hazardous nature of pesticides, poor hygiene practices, lack of availability of washing facilities, and insufficient adherence to precautionary instructions on pesticide labels. Mitigating the risks posed by pesticides is considered a less compelling interest than alleviating poverty. Women working in horticulture in Tanzania usually have low levels of education and income and lack decision-making power even on matters relating to their own health. This contributes to pesticide exposure and other health challenges. Because of multiple factors, some of which act as study confounders, few studies on exposure to pesticides and health effects have been conducted among women. This review identified factors that contribute to the increased health effects among women working in the horticultural industry and how these effects relate to pesticide exposure.

show abstract

Section: Pesticide Use In Horticulturementioning

confidence: 99%

Pesticide Exposure and Health Problems Among Female Horticulture Workers in Tanzania

Mrema

Ngowi

Kishinhi

et al. 2017

Environ�Health�Insights

View full text Add to dashboard Cite

show abstract

“…Human ES cells were treated with E2 (10 -8 M), P4 (10 -6 M), ICI 182,780 (10 -6 M) or RU486 (10 -8 M) for 48 h. Cells were harvested with RIPA buffer (50 mM Tris-HCl, pH 8.0; 150 mM NaCl, 1% Triton X-100 (Sigma-Aldrich), 0.5% deoxycholic acid (Sigma-Aldrich), and 0.1% SDS. Western blot analysis was performed as previously described (25). Briefly, protein concentration was determined by a bicinchoninic acid (Sigma-Aldrich) assay.…”

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Altered expression of epithelial mesenchymal transition and pluripotent associated markers by sex steroid hormones in human embryonic stem cells

Jeon

Hwang

Kim

et al. 2017

Molecular Medicine Reports

Self Cite

View full text Add to dashboard Cite

Embryonic stem (ES) cells are pluripotent stem cells derived from a developmental stage of pre‑implanted embryos. The present study investigated the effect of female sex steroid hormones on the characteristics of human ES cells by using a feeder‑free culture protocol. In a feeder‑free condition without sex hormones, human ES cells assumed the form of tightly packed cells that grow in a monolayer. The cells had clean and defined edges with no evidence of differentiation and expressed several markers specific for undifferentiated ES cells including POU class 5 homeobox 1 (POU5F1), sex determining region Y‑box 2 (SOX2) and NANOG homeobox (NANOG). It was then investigated if female sex steroid hormones including 17β‑estradiol (E2) and progesterone (P4) altered the protein expression of epithelial-mesenchymal transition (EMT) associated markers in addition to pluripotency markers including POU5F1, SOX2 and NANOG in human ES cells. The protein expression levels of N‑cadherin, Snail and Slug were increased while E‑cadherin expression was decreased by treatment of E2 or P4, and the expression levels of POU5F1, SOX2 and NANOG were decreased by the treatment of E2 or P4. When E2 and P4 were treated in combination with an estrogen receptor inhibitor (ICI 182,780) and progesterone receptor inhibitor (RU486) respectively, their effects on EMT and pluripotency of ES cells were restored to control levels. The results suggested that E2 and P4 may regulate EMT and pluripotency of human ES cells by mediating their receptors. The present study may aid in the understanding of the role of sex steroid hormones in the cellular biology of human ES cells.

show abstract

“…Endocrine-disrupting chemicals (EDCs) are exogenous chemical compounds which can interfere with hormone balance and the normal functions of endocrine system [ 1 – 2 ] . Generally, EDCs bind to hormone receptors such as the androgen receptor (AR) and estrogen receptor (ER) and thereby interrupt the functions of endogenous steroid hormones [ 3 ] .…”

Section: Introductionmentioning

confidence: 99%

Effects of bisphenol compounds on the growth and epithelial mesenchymal transition of MCF-7 CV human breast cancer cells

et al. 2017

View full text Add to dashboard Cite

Bisphenol-A (BPA) has been considered as an endocrine disrupting chemical (EDC) because it can exert estrogenic properties. For bisphenol-S (BPS) and bisphenol-F (BPF) that are BPA analogs and substitutes, their risk to estrogen-dependent cancer has been reported rarely compared with the numerous cases of BPA. In this study, we examined whether BPA, BPS, and BPF can lead to the proliferation, migration, and epithelial mesenchymal transition (EMT) of MCF-7 clonal variant (MCF-7 CV) breast cancer cells expressing estrogen receptors (ERs). In a cell viability assay, BPA, BPS, and BPF significantly increased proliferation of MCF-7 CV cells compared to control (DMSO) as did 17β-estradiol (E2). In Western blotting assay, BPA, BPS, and BPF enhanced the protein expression of cell cycle progression genes such as cyclin D1 and E1. In addition, MCF-7 CV cells lost cell to cell contacts and acquired fibroblast-like morphology by the treatment of BPA, BPS, or BPF for 24 hours. In cell migration assay, BPA, BPS, and BPF accelerated the migration capability of MCF-7 CV cells as did E2. In relation with the EMT process, BPA, BPS, and BPF increased the protein expression ofN-cadherin, while they decreased the protein expression of E-cadherin. When BPA, BPS, and BPF were co-treated with ICI 182,780, an ER antagonist, proliferation effects were reversed, the expression of cyclin D1 and cyclin E1 was downregulated, and the altered cell migration and expression ofN-cadherin and E-cadherin by BPA, BPS, and BPF were restored to the control level. Thus, these results imply that BPS and BPF also have the risk of breast cancer progression as much as BPA in the induction of proliferation and migration of MCF-7 CV cells by regulating the protein expression of cell cycle-related genes and EMT markersvia the ER-dependent pathway.

show abstract

Treatment of BG-1 Ovarian Cancer Cells Expressing Estrogen Receptors with Lambda-cyhalothrin and Cypermethrin Caused a Partial Estrogenicity Via an Estrogen Receptor-dependent Pathway

Cited by 16 publications

References 26 publications

Pesticide Exposure and Health Problems Among Female Horticulture Workers in Tanzania

Pesticide Exposure and Health Problems Among Female Horticulture Workers in Tanzania

Altered expression of epithelial mesenchymal transition and pluripotent associated markers by sex steroid hormones in human embryonic stem cells

Effects of bisphenol compounds on the growth and epithelial mesenchymal transition of MCF-7 CV human breast cancer cells

Contact Info

Product

Resources

About