Treatment of benign prostatic hyperplasia with Croton membranaceus in an experimental animal model

Afriyie, Daniel Kwame; Asare, George Awuku; Bugyei, Kwasi Agyei; Adjei, Samuel; Lin, Jiumao; Peng, Jun; Zhang, Hong

doi:10.1016/j.jep.2014.09.007

Cited by 26 publications

(25 citation statements)

References 44 publications

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“…1) The human prostate has two major cell types: glandular cells (secretory acini), which consist of columnar and basal cells, and dense stromal cells, which mainly consist of collagen and smooth muscle cells. 2) BPH is characterized by the progressive growth of prostatic glandular epithelial and stromal elements, culminating in uncontrolled hyperplasia. 3) As the prostate enlarges, it tightens the urethra, prompting a weak urinary stream, incomplete bladder emptying, dysuria, nocturia, and bladder outlet obstruction.…”

Section: Introductionmentioning

confidence: 99%

Effect of <i>Veratrum maackii</i> on Testosterone Propionate-Induced Benign Prostatic Hyperplasia in Rats

Park

Seo

Wijerathne

et al. 2019

Biological & Pharmaceutical Bulletin

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Veratrum maackii (VM), a perennial plant in the Melanthiaceae family, has anti-hypertensive, anticholinergic, anti-asthmatic, anti-tussive, anti-fungal, anti-melanogenesis, and anti-tumor activities. Here, we investigated the therapeutic effect of VM on benign prostatic hyperplasia (BPH) in human normal prostate cell line (WPMY-1) and a testosterone propionate-induced BPH animal model. WPMY-1 cells were treated with VM (1-10 µg/mL) and testosterone propionate (100 nM). BPH in rats was generated via daily subcutaneous injections of testosterone propionate (3 mg/kg) dissolved in corn oil, for 4 weeks. VM (150 mg/kg) was administered daily for 4 weeks by oral gavage concurrently with the testosterone propionate. All rats were sacrificed and the prostates were dissected, weighed, and subjected to histological, immunohistochemical, and biochemical examinations. Immunoblotting experiments indicated that WPMY-1 cells treated testosterone propionate had increased expression of prostate specific antigen (PSA) and androgen receptor (AR), and treatment with VM or finasteride blocked this effect. In rat model, VM significantly reduced prostate weight, prostatic hyperplasia, prostatic levels of dihydrotestosterone (DHT), and expression of proliferation markers such as proliferating cell nuclear antigen (PCNA) and cyclin D1, but increased the expression of pro-apoptotic Bcl-2-associated X protein (Bax) and the cleavage of caspase-3. VM administration also suppressed the testosterone propionate-induced activation of nuclear factor-kappaB (NF-κB). Our results indicate that VM effectively represses the development of testosterone propionateinduced BPH, suggesting it may be a useful treatment agent for BPH.

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Section: Introductionmentioning

confidence: 99%

Effect of <i>Veratrum maackii</i> on Testosterone Propionate-Induced Benign Prostatic Hyperplasia in Rats

Park

Seo

Wijerathne

et al. 2019

Biological & Pharmaceutical Bulletin

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“…Testosterone has been widely used as an inducer of benign prostatic hyperplasia (BPH) in animal models in order to test the protections of various natural products. 29 Hyperplasia models have been characterized by increased prostate volume, as well as alterations in prostatic tissues including enlargement of the glandular cavity, proliferation of prostatic epithelium and stromal cells and infiltration of inflammatory cells, being a pathological index of BPH. 30 In this study, all animals that received testosterone developed BPH, except for the group that received dutasteride ( Figure 3C).…”

Section: Discussionmentioning

confidence: 99%

Protective Effect of the Ethanolic Extracts of Leaves of Chuquiraga spinosa Less and Baccharis genistelloides on Benign Prostatic Hyperplasia in Rats

Palomino-De-La-Gala

Justil-Guerrero

Arroyo-Acevedo

et al. 2019

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The phytochemical studies of Chuquiraga spinosa Less have revealed that it contains 9 types of flavonoids (quercetin-3-O-glucuronide, quercetin-3-O-rutinoside, quercetin-3-O-glucoside, kaemperol-3-O-glucuronide, kaemperol-3-O-rutinoside, kaempherol-3-O-glucoside, isorhamnetin-3-Oglucuronide, isorhamnetin-3-O-rutinoside and isorhamnetin-3-O-glucoside) and a phenolic compound (p-hydroxyacetophenone), flavonoids ABSTRACT Introduction: Benign prostatic hyperplasia (BPH) is one of the most common conditions affecting middle-aged men, with oxidative stress postulated as an important factor in its development. Objective: To evaluate the protective effect of the association of the ethanolic extract of Chuquiraga spinosa (ChS) and Baccharis genistelloides (BaG) on benign prostatic hyperplasia in rats. Materials and Methods: Sixty-six male rats were randomized into groups: 1) Inductor Group: Testosterone enanthate (ET) 25 mg/Kg on days 8 and 14 intramuscularly. 2) Group P80: Polysorbate-80 3% 10 mL / Kg. 3) Positive Group: ET + Dutasteride (DU) 0.5 mg / Kg,

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“…BPH is a noncancerous prostate condition, which is caused by the overgrowth of prostatic epithelial and stromal cells, and arises due to the imbalance between proliferation and apoptosis of prostatic cells [22]. Increased proliferation of epithelial and stromal cells in the prostate cause the overall enlargement of the prostate gland, which is a signal of BPH development [23, 24].…”

Section: Discussionmentioning

confidence: 99%

Assortment of Stauntonia hexaphylla and Cornus officinalis protect against testosterone-induced benign prostatic hyperplasia through anti-inflammatory and anti-proliferative activity

Karunasagara

Hong

Jung

et al. 2019

Preprint

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Benign prostatic hyperplasia (BPH) is a progressive pathological condition associated with proliferation of prostatic tissues, prostate enlargement, and lower-urinary tract symptoms. However, the mechanism underlying the pathogenesis of BPH is not clear. The aim of this study was to investigate the protective effects of Stauntonia hexaphylla and Cornus officinalis (SC extract) on a testosterone propionate (TP)-induced BPH model. For in vitro experiments, a human prostate adenocarcinoma cell line was used to perform western blotting for androgen receptor (AR), prostate specific antigen (PSA), and 5α-reductase type 2. Male Sprague-Dawley rats were randomly divided into 8 groups as follows for the in vivo experiments: control, BPH, Fina, Saw, SC25, SC50, SC100, and SC200. To induce BPH, all rats, except those in the control group, were daily administered with subcutaneous injections of TP (5 mg/kg), and orally treated with appropriate PBS/drugs for 4 consecutive weeks. Our findings indicated that the SC treatment significantly reduced the prostate size and downregulated the serum testosterone and DHT levels in BPH rats. The histological examination revealed that SC treatment markedly recovered the TP-induced abnormalities and reduced the prostatic hyperplasia. In addition, in vitro and in vivo western blotting indicated that SC treatment significantly downregulated the AR, PSA, and 5α-reductase type 2 expression, while an immunohistochemistry examination revealed that the SC extract significantly reduced the expression of type 2 5α-reductase and proliferating cell nuclear antigen positive cell count. Collectively, our findings demonstrated that SC extract attenuates BPH through anti-proliferative and anti-inflammation activities and might be useful in the clinical treatment of BPH.

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Treatment of benign prostatic hyperplasia with Croton membranaceus in an experimental animal model

Cited by 26 publications

References 44 publications

Effect of <i>Veratrum maackii</i> on Testosterone Propionate-Induced Benign Prostatic Hyperplasia in Rats

Effect of <i>Veratrum maackii</i> on Testosterone Propionate-Induced Benign Prostatic Hyperplasia in Rats

Protective Effect of the Ethanolic Extracts of Leaves of Chuquiraga spinosa Less and Baccharis genistelloides on Benign Prostatic Hyperplasia in Rats

Assortment of Stauntonia hexaphylla and Cornus officinalis protect against testosterone-induced benign prostatic hyperplasia through anti-inflammatory and anti-proliferative activity

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