Treatment of antebrachial and crural septic nonunion fractures in dogs using circular external skeletal fixation: a retrospective study

Cappellari, Fulvio; Piras, Lisa Adele; Panichi, E; Ferretti, Andrea; Peirone, Bruno

doi:10.3415/vcot-13-08-0102

Cited by 11 publications

(15 citation statements)

References 34 publications

(60 reference statements)

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“…Several animal models have been used to study fracture nonunion or delayed union (Chu et al, 2009;Lozada-Gallegos et al, 2013;Cappellari et al, 2014;Shimizu et al, 2015). In recent years, fracture research has focused on rats and mice (Garcia et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Glycyrrhizic Acid Promotes Osteogenic Differentiation of Human Bone Marrow Stromal Cells by Activating the Wnt/β-Catenin Signaling Pathway

Bai

Hang

et al. 2021

Front. Pharmacol.

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Glycyrrhizic acid (GA) is a major triterpene glycoside isolated from liquorice root that has been shown to inhibit osteoclastogenesis. However, there have been no reports regarding the effect of GA on osteogenic differentiation. Therefore, this study was performed to explore the effects and mechanism of action of GA on osteogenesis. A CCK-8 array was used to assess cell viability. The osteogenic capability was investigated by real-time quantitative PCR, western blotting and immunofluorescence analyses. ALP staining and ARS were used to evaluate ALP activity and mineralization, respectively. GA-GelMA hydrogels were designed to verify the therapeutic effects of GA in vivo by radiographic analysis and histological evaluation. Our results show that GA had no significant influence on the viability or proliferation of human bone marrow stromal cells (hBMSCs). GA promoted osteogenic differentiation and enhanced calcium deposition. Furthermore, ratio of active β-catenin and total β-catenin protein increased after treatment with GA. Wnt/catenin signaling inhibitor partially attenuated the effects of GA on osteogenic differentiation. In a mouse femoral fracture model, GA-GelMA hydrogels accelerated bone healing. Our results show that GA promotes the osteogenic differentiation of hBMSCs by modulating the Wnt/β-catenin signaling pathway. GA-GelMA hydrogels promoted bone fracture healing. GA has potential as a cost-effective treatment of bone defects.

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Section: Discussionmentioning

confidence: 99%

Glycyrrhizic Acid Promotes Osteogenic Differentiation of Human Bone Marrow Stromal Cells by Activating the Wnt/β-Catenin Signaling Pathway

Bai

Hang

et al. 2021

Front. Pharmacol.

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“…As such, grafting or collapsing the ostectomy site is generally recommended when bone length loss is <20%, and more elaborate options such as bone-transport osteogenesis should be considered when bone length loss is >20%. 4,14 Our cases had defects spanning 20% and 32% of the original bone length, and union was documented in less than 3 months. The cases described in our report were chronically and severely diseased, yet progression of graft incorporation resembled the healing qualities observed in normal healthy bone.…”

Section: Discussionmentioning

confidence: 81%

“…[1][2][3] Other methods such as bone transport osteogenesis and microvascular free bone transfer require high levels of training, are technically demanding and resource intensive, and may require prolonged convalescence. [3][4][5][6] Because of the limitations of the techniques that are described above, biologic treatments based on known cellular and molecular pathways of bone formation have garnered tremendous interest. 7 Bone morphogenetic proteins (BMP) are potent osteoinductive cytokines, and BMP-2 and BMP-7 are commercially available for therapeutic use in man.…”

Section: Introductionmentioning

confidence: 99%

Human placenta‐derived matrix with cancellous autograft and demineralized bone matrix for large segmental long‐bone defects in two dogs with septic nonunion

2020

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To report the successful treatment of septic nonunion in two dogs with large segmental defects secondary to long-bone fractures by using a novel human placenta-derived matrix (hPM) as adjunct to fixation. Animals.: One 3-kg 9-year-old neutered male Yorkshire terrier with a distal antebrachial fracture and one 6-kg 4-year-old spayed female miniature pinscher with a distal humeral fracture. Study design.: Short case series. Methods: Both dogs presented for septic nonunion after internal fixation of Gustilo type II open diaphyseal fractures from dog bite injuries. During revision, debridement of nonviable bone resulted in segmental defects of 32% and 20% of the bone length for the antebrachial and humeral fractures, respectively. The antebrachial fracture was stabilized with a circular external fixator, and the humeral fracture was stabilized with biaxial bone plating. The fracture sites were not collapsed, and full length was maintained with the fixation. Autogenous cancellous bone graft and canine demineralized bone allograft were packed into the defects, and hPM was injected into the graft sites after closure. Results: Radiographic union was documented at 8 weeks and 6 weeks for the antebrachial and humeral fractures, respectively. Both dogs became fully weight bearing on the affected limbs and returned to full activity. Conclusion: Augmenting fixation with grafts and hPM led to a relatively rapid union in both dogs reported here.

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“…Entretanto, alguns estudos tiveram como critério de inclusão o peso dos cães: estudos por Aikawa et al, (2018) Nemanic, 2018). ≤ 5 kg (Piras et al, 2011), ≤ 4kg (Ramírez; Macías, 2016), ≤ 3,5kg (Manchi et al, 2017), ≤ 2,5kg (Kang et al, 2016) Causas das fraturas % meses ou mais (Gibert et al,2015). As fraturas de rádio e ulna estudadas em sua maioria acometeram animais jovens e de pequeno porte.…”

Section: Methodsunclassified

“…O tempo entre a lesão até o momento do procedimento cirúrgico variou nos estudos de 1 a 15 dias, com tempo médio de 6,48 ± 5,36 dias (Piras et al, 2011;Bierens et al, 2017;Ramírez;Macías, 2016;De Arbun Parent et al, 2017).…”

Section: Methodsunclassified

Desenvolvimento de uma membrana sintética na regeneração óssea

Anjos¹

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Os reparos de perdas ósseas, como no caso de fraturas, representam um desafio na rotina cirúrgica ortopédica, uma vez que exige o desenvolvimento de sistemas que contribuem para promoção da regeneração do tecido perdido, permitindo o completo retorno à função. A utilização de enxertos autógenos e alógenos é uma alternativa de tratamento para essas situações, entretanto existem os riscos de infecções cirúrgicas, dificuldade para reposicionamento do animal no trans-cirúgico, da dor pós-operatória, e a quantidade do material coletado, na maioria das vezes, torna-se insuficiente para realização do procedimento. Por isso, as pesquisas e novos métodos, como a engenharia de tecidos ósseos e medicina regenerativa, visam aumentar sua disponibilidade, reduzir o tempo de recuperação, os custos relacionados e melhorar a regeneração tecidual. Neste contexto, as terapias com biomateriais sintéticos e biodegradáveis como biocerâmicas e polímeros demonstram um grande potencial para a regeneração óssea. Entretanto, existe um grande desafio ao se combinar diferentes materiais a fim de obter a interação desses com o meio biológico e minimizar reações indesejáveis, adequando o tempo de degradação e a taxa de crescimento tecidual no local de implantação. Além da combinação adequada dos materiais, o método de processamento para a obtenção de matrizes porosas é importante. Os materiais de fosfato de cálcio são comumente usados na engenharia de tecidos ósseos, e desses materiais, a hidroxiapatita (HAP) é frequentemente usada, pois é o principal componente dos ossos, com capacidade osseocondutora. Já a policaprolactona (PCL) é um dos polímeros mais atraentes para regeneração óssea, com excelentes propriedades mecânicas, biocompatibilidade e biodegradabilidade. Dessa forma, pretendeu-se com esse trabalho realizar três capítulos: O primeiro que é avaliar a influência dos tipos de tratamentos e fatores predisponentes a complicações na consolidação de fraturas de rádio e ulna em cães por meio de uma revisão sistemática; O segundo, obter o perfil epidemiológico de cães com fraturas de rádio e ulna atendidos no hospital veterinário-ufv; e por último, desenvolver e caracterizar uma membrana de hidroxiapatita e policaprolactona e analisar sua utilização na regeneração óssea em modelo experimental de ostectomia de rádio em coelhos. Palavras-chave: Medicina Regenerativa. Engenharia de Tecidos. Biomateriais.

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Treatment of antebrachial and crural septic nonunion fractures in dogs using circular external skeletal fixation: a retrospective study

Cited by 11 publications

References 34 publications

Glycyrrhizic Acid Promotes Osteogenic Differentiation of Human Bone Marrow Stromal Cells by Activating the Wnt/β-Catenin Signaling Pathway

Glycyrrhizic Acid Promotes Osteogenic Differentiation of Human Bone Marrow Stromal Cells by Activating the Wnt/β-Catenin Signaling Pathway

Human placenta‐derived matrix with cancellous autograft and demineralized bone matrix for large segmental long‐bone defects in two dogs with septic nonunion

Desenvolvimento de uma membrana sintética na regeneração óssea

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