Treatment-independent miRNA signature in blood of wilms tumor patients

Abstract

BackgroundBlood-born miRNA signatures have recently been reported for various tumor diseases. Here, we compared the miRNA signature in Wilms tumor patients prior and after preoperative chemotherapy according to SIOP protocol 2001.ResultsWe did not find a significant difference between miRNA signature of both groups. However both, Wilms tumor patients prior and after chemotherapy showed a miRNA signature different from healthy controls. The signature of Wilms tumor patients prior to chemotherapy showed an accuracy of 97.5% and of patients after chemotherapy an accuracy of 97.0%, each as compared to healthy controls.ConclusionOur results provide evidence for a blood-born Wilms tumor miRNA signature largely independent of four weeks preoperative chemotherapy treatment.

Biomarkers to Detect Wilms Tumors in Pediatric Patients: Where are We Now?

“…A method for circumventing this problem has been proposed; the liquid biopsy. This approach appears extremely promising in other cancers and at present, both epigenetically modified DNA and miRNAs have been detected in the circulation of patients with WT, however these were small pilot studies and the full potential for this method is yet to be explored [31,43,55]. As both genetic and epigenetic mutations can be detected in DNA released from tumors, this may warrant an exciting avenue for further tracking of tumors including early i dentification of relapse.…”

“…Finally, a miRNA profile distinct to high-risk pretreated WTs was identified, of which seven miRNAs were also highly expressed at the prechemotherapy biopsy stage and may therefore be used to identify risk group in advance [30]. Each of these studies were carried out in tumor tissue, however, a further study assessed miRNA content within blood of patients treated with preoperative chemotherapy and identified a signature within patients, not associated with chemotherapy, that was distinct from healthy individuals [31]. This proves that miRNA detection in the circulation for WT patients is feasible and may be important for diagnosis.…”

The role of microRNAs in the pathogenesis of MMPi-induced skin fibrodysplasia

“…These were selected on the basis of their high level of correlation with evidence of FD, and the availability of experimentally validated assays for the canine. Receiver operator curve (ROC) analysis was performed and area under the curve (AUC) values computed to ascertain the ability of each prospective biomarker to correctly identify diseased and disease-free animals [19]. The ability of these potential biomarkers to discriminate between the various severities of FD was not examined due to the limited number of cases in each category.…”

“…ROC analysis was performed on data from all study time points and area under the curve (AUC) values were computed using GraphPad 5.0 software (Prism) as an index of discriminatory power. An AUC value of 0.5 denotes no ability to correctly identify diseased and disease-free subjects, whilst an AUC value of 1.0 denotes perfect decimation between the two groups of interest [19]. …”

The translational potential of microRNAs as biofluid markers of urological tumours

“…[65][66][67][68][69][70][71][72][73][74][75][76] Specifically, relatively few studies, including only one multicentre study 67 , have been conducted, and most studies did not perform an internal validation with a training and validation set or even an internal validation approach, such as bootstrapping or cross-validation. Moreover, all studies except one used serum as the source of RNA despite plasma being the blood component of choice since the release of miRNAs from blood cells such as the miRNA-enriched platelets into serum occurs during the coagulation process.…”