Treatment effect of l-Norvaline on the sexual performance of male rats with streptozotocin induced diabetes

De, Abhijit; Singh, Manju; Singh, Vinod; Ram, Veerma; Bisht, Sandeep

doi:10.1016/j.ejphar.2015.12.008

Cited by 35 publications

(30 citation statements)

References 47 publications

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“…As reported earlier, intraperitoneal administration of single dose of streptozotocin significantly enhanced the serum glucose level in fasted male rats [12,19,20]. High diabetic condition had empowered the level of not only glucose but also the production of free radicals that induced testicular damage and vascular impairment [21,22].…”

Section: Discussionmentioning

confidence: 58%

“…This enzyme in turn accelerates the Rho activation and ultimately over expression of oxygen free radicals. Peroxynitrates may be generated due to administration of L-Norvaline which is indirectly a nitric oxide donor [12]. Prolonged administration of this drug may cause accumulation of higher concentration of nitric oxide and accelerates the cavernosal smooth muscle proliferation.…”

Section: Discussionmentioning

confidence: 99%

“…Multiple comparisons between different groups was performed using Analysis of Variance (ANOVA) followed by Dunetts Multiple comparison Test. Difference level at P<0.05 was considered as statistically significant [12].…”

Section: Discussionmentioning

confidence: 99%

“…Fasting serum glucose (FSG) level was checked by glucose oxidase-peroxidase method using Seimen Diagnostic Kits. Animals showing FSG level above 250 mg/dL were considered as diabetic and included for the study [12].…”

Section: Induction Of Diabetes Mellitusmentioning

confidence: 99%

“…It is a white crystalline powder with melting point 303°C to 307°C and well soluble in water (10.5 g/100 mL), very soluble in hot water and dilute hydrochloric acid. De and his co-workers had established the fact that L-Norvaline is a well established arginase inhibitor when administered in diabetic male rats [12].…”

Section: Introductionmentioning

confidence: 99%

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L-Norvaline and Alpha-tocopherol Treatment Protect Against Diabetes-Induced Oxidative Stress in Testes of Male Rats

De¹,

Singh²

2016

Reprod Syst Sex Disord

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The present study aimed to evaluate the effect of L-Norvaline and alpha-tocopherol in protection and combating the oxidative stress of testes in hyperglycemic male rats. In our study single dose of streptozotocin was used for induction of diabetes and fasting serum glucose level was checked and those animals showing FSG above 250 mg/dL were selected. The diabetic animals were randomly divided into seven groups comprising six animals in each group and one group of normal non diabetic animals received the vehicle 0.1 M citrate buffer pH 4.5. L-Norvaline was administered at higher (10 mg/kg) and lower (8 mg/kg) dose intraperitoneally to the different groups of diabetic animals along with alpha-tocopherol (900 mg/kg) orally for a period of 30 days. Sildenafil at an oral dose of 5 mg/kg was used as reference drug. After that period, testis was isolated and homogenized. The homogenized tissue was centrifuged, separating the supernatant and preserved it for assessing the oxidative stress parameters. The level of various antioxidant enzymes were assessed such as superoxide dismutase, reduced glutathione, glutathione peroxidase and glutathione reductase. Assessment of lipid peroxidation was also done in treated groups. The statistical analyses were carried out using Graph Pad Prism 5.0 software. All values were presented as Mean ± S.E.M. The level of superoxide dismutase, reduced glutathione, glutathione peroxidase and glutathione reductase were highly diminished in the diabetic rats compared with normal group. L-Norvaline alone showed no significant improvement in those enzymes parameters except in reduced glutathione (P<0.05). L-Norvaline both at higher dose and lower dose along with alpha-tocopherol showed significant improvement in the level of antioxidant enzymes as compared with diabetic group (P<0.05, P<0.01, P<0.001). Treated groups also showed a significant decrease in the level of malondialdehyde as compared to diabetic group (P<0.01, P<0.001). Standard drug, Sildenafil showed no improvement in any parameters after 30 days. The present can be concluded by establishing the fact that LNorvaline and Alpha-tocopherol could be a good combination in scavenging free radicals in testes due to streptozotocin induced hyperglycemia in male Wistar rats.

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Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Induction Of Diabetes Mellitusmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

L-Norvaline and Alpha-tocopherol Treatment Protect Against Diabetes-Induced Oxidative Stress in Testes of Male Rats

De¹,

Singh²

2016

Reprod Syst Sex Disord

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Acute hypoxia elevates arginase 2 and induces polyamine stress response in zebrafish via evolutionarily conserved mechanism

Banerjee

Khrystoforova

Polis

et al. 2021

Cell. Mol. Life Sci.

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Living organisms repeatedly encounter stressful events and apply various strategies to survive. Polyamines are omnipresent bioactive molecules with multiple functions. Their transient synthesis, inducible by numerous stressful stimuli, is termed the polyamine stress response. Animals developed evolutionarily conserved strategies to cope with stresses. The urea cycle is an ancient attribute that deals with ammonia excess in terrestrial species. Remarkably, most fish retain the urea cycle genes fully expressed during the early stages of development and silenced in adult animals. Environmental challenges instigate urea synthesis in fish despite substantial energetic costs, which poses the question of the urea cycle's evolutionary significance. Arginase plays a critical role in oxidative stress-dependent reactions being the final urea cycle enzyme. Its unique subcellular localization, high inducibility, and several regulation levels provide a supreme ability to control the polyamine synthesis rate. Notably, oxidative stress instigates the arginase-1 activity in mammals. Arginase is also dysregulated in aging organisms' brain and muscle tissues, indicating its role in the pathogenesis of age-associated diseases. We designed a study to investigate the levels of the urea cycle and polyamine synthesis-related enzymes in a fish model of acute hypoxia. We evidence synchronized elevation of arginase-2 and ornithine decarboxylase following oxidative stress in adult fish and aging animals signifying the specific function of arginase-2 in fish. Moreover, we demonstrate oxidative stress-associated polyamine synthesis' induction and urea cycle' arrest in adult fish. The subcellular arginase localization found in the fish seems to correspond to its possible evolutionary roles.

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Ameliorative effects of Lycium chinensis on male sexual dysfunction and testicular oxidative damage in streptozotocin-induced diabetic rats

Zhou

Olatunji

Chen

2019

Food Sci Biotechnol

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The root bark of Lycium chinense (LC) has been reported to have potent antioxidant and antidiabetic properties. In the present study, we investigated the attenuative effect of LC against diabetes induced sexual dysfunction and testicular damages in animal models. Diabetic animals were treated with LC (100 and 400 mg/kg) once daily for 6 weeks. At the end of the treatment, mating behavior tests were performed and the animals were sacrificed for the determination of hormonal profile, oxidative stress indices and sperm analysis. LC administration significantly decreased blood glucose level, enhancement of the antioxidant enzyme activities, restored altered sperm characteristics and markedly improved levels of luteinizing hormone, follicle-stimulating hormone and testosterone as compared to the untreated diabetic animals. Furthermore, LC also improved sperm count, viability, motility, increased the reproductive organs weight. The results obtained indicated that L. chinense has beneficial effects in diabetes sexual dysfunction.

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Treatment effect of l-Norvaline on the sexual performance of male rats with streptozotocin induced diabetes

Cited by 35 publications

References 47 publications

L-Norvaline and Alpha-tocopherol Treatment Protect Against Diabetes-Induced Oxidative Stress in Testes of Male Rats

L-Norvaline and Alpha-tocopherol Treatment Protect Against Diabetes-Induced Oxidative Stress in Testes of Male Rats

Acute hypoxia elevates arginase 2 and induces polyamine stress response in zebrafish via evolutionarily conserved mechanism

Ameliorative effects of Lycium chinensis on male sexual dysfunction and testicular oxidative damage in streptozotocin-induced diabetic rats

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