Treating maize plants with benzohydrazide increases saccharification of lignocellulose: A non-transgenic approach to improve cellulosic ethanol production

Martarello, Danielly Caroline Inacio; Tonete-Diniz, Débora Carvalho; Gonzaga, Diego Eduardo Romero; Almeida, Aline Marengoni; Constantin, Renato Polimeni; Silva, Karla Gabriela da; Constantin, Rodrigo Polimeni; Marchiosi, Rogério; Alves-Olher, Vanessa Guimarães; Rios, Fabiano Aparecido; Ferrarese‐Filho, Osvaldo; Santos, Wanderley Dantas dos

doi:10.1007/s13399-021-01842-x

Cited by 1 publication

(2 citation statements)

References 45 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They commonly inhibit enzymes of the phenylpropanoid pathway and are rapidly metabolized in both plants and soil. Despite this, they persistently enhance saccharification (up to 10 months of postapplication) in a diverse range of plant species, including soybean, Brachiaria decumbens , sugarcane (dos Santos et al., 2023), and maize (Martarello et al., 2023). Consequently, these inhibitors could play a significant role in the burgeoning cellulosic ethanol industry.…”

Section: Discussionmentioning

confidence: 99%

“…Benzohydrazide (BHZ) was selected from a set of phenolic derivatives screened in bench‐scale for their ability to induce lignocellulose saccharification without affecting productivity. We then tested it in field‐scale unrevealing that young maize crops (30 days old) sprayed with BHZ induce a long‐term change in lignocellulose that turn mature plants lignocellulose substantially prone for saccharification with or without pretreatment for removal of lignin (HPAC) with any impact on maize productivity (Martarello et al., 2023).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Natural lignin modulators improve bagasse saccharification of sugarcane and energy cane in field trials

de Oliveira,

Figueiredo,

de Oliveira Correia

et al. 2023

GCB Bioenergy

View full text Add to dashboard Cite

The burgeoning cellulosic ethanol industry necessitates advancements in enzymatic saccharification, effective pretreatments for lignin removal, and the cultivation of crops more amenable to saccharification. Studies have demonstrated that natural inhibitors of lignin biosynthesis can enhance the saccharification of lignocellulose, even in tissues generated several months post‐treatment. In this study, we applied daidzin (a competitive inhibitor of coniferaldehyde dehydrogenase), piperonylic acid (a quasi‐irreversible inhibitor of cinnamate 4‐hydroxylase), and methylenedioxy cinnamic acid (a competitive inhibitor of 4‐coenzyme A ligase) to 60‐day‐old crops of two conventional Brazilian sugarcane cultivars and two energy cane clones, bred specifically for enhanced biomass production. The resultant biomasses were evaluated for lignin content and enzymatic saccharification efficiency without additional lignin‐removal pretreatments. The treatments amplified the production of fermentable sugars in both the sugarcane cultivars and energy cane clones. The most successful results softened the most recalcitrant lignocellulose to the level of the least recalcitrant of the biomasses tested. Interestingly, the softest material became even more susceptible to saccharification.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%