Treating donor plants with 2,4-dichlorophenoxyacetic acid can increase the effectiveness of induced androgenesis in Capsicum spp.

Nowaczyk, L.; Nowaczyk, P.; Olszewska, Dorota

doi:10.1016/j.scienta.2016.03.044

Cited by 10 publications

(6 citation statements)

References 18 publications

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“…This study confirms the effectiveness of microspore embryogenesis for the generation of haploid/DH embryos and homozygous regenerants in pepper through anther culture; however, many factors were observed that influenced the effectiveness of anther culture. These findings are in alignment with previous findings that reported the effect of donor plants (Cheng et al 2013), microspore developmental stage (Parra-Vega et al 2013a), culture medium (Irikova et al 2011a;Nowaczyk et al 2016;Ata et al 2019;, growing conditions and age (Irikova et al 2011b;Parra-Vega et al 2013b;Popova et al 2016;. Niklas-Nowak et al (2012) observed that the significant differences were seen in the androgenic response of tested genotypes of individual plants and confirmed that the donor plant genotype is a critical factor in determining the effectiveness of androgenesis in pepper, which is in coherence with our findings.…”

Section: Discussionsupporting

confidence: 93%

“…Morphological characterization of plants is a possibility not only to prove the spontaneous genome diploidization and achieving homozygosity, but also to evaluate the breeding potential of prospective lines. Observed results for agronomic and morphological traits showed noticeable variation for fruit morphological and productivity traits between diverse androgenic lines of similar background and these results are in accordance with and Nowaczyk et al (2016). Variation for fruit morphological and agronomical traits observed between genotypes of Stryama, Zlaten medal 7, and Victoria varieties background and similar observations have been observed in previous studies in regard to fruit morphological traits variation among androgenic lines of similar background (Shrestha et al 2011;Luitel et al 2012;Shmykova et al 2014;Trajkova and Koleva-Gudeva 2017).…”

Section: Discussionsupporting

confidence: 90%

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Creation of pepper doubled haploids and morphological characterization of androgenic plants

2021

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The Balkan pepper breeding program aims to use in vitro embryogenesis (anther culture) to develop doubled haploid pepper lines with valuable traits. We analyzed the effectiveness of microspore embryogenesis on 17 pepper genotypes comprised of varieties, breeding lines, and F1 hybrids of different varietal types (conical, bell shape, and kapia) and characterized the agronomic traits of newly generated doubled haploid lines. The highest androgenic potential was observed in the variety Stryama and breeding line 560/06 with 9.55 and 13.00% reacted anthers, respectively. Of the 186 regenerated plants, 147 were successfully adapted with a survival index of 79.03%. Flow cytometry analysis showed that the haploid:diploid ratio of regenerants was 1.5:1. All diploid plants were confirmed to be androgenic in origin. There were significant differences in terms of quantitative fruit characteristics of length, width, weight, pericarp weight, pericarp thickness, and productivity per plant among the diploid lines. Also, androgenic lines 21, 23, and 74 derived from Stryama and line 55 obtained from Zlaten medal 7 were distinguished with higher values of some fruit traits compared to initial genotypes. Additionally, several androgenesis lines (Stryama lines 21, 23, and 74, and Zlaten medal 7 line 55) scored higher for some fruit traits compared to the initial genotype. These results show that anther culture is a promising tool for the creation of Balkan pepper breeding lines with improved traits.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 90%

Creation of pepper doubled haploids and morphological characterization of androgenic plants

2021

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“…Further, M10 media was supplemented with 2,4-D, whereas M11 media had 2,4-D and PVP. The addition of 2,4-D to the anther culture medium increased the effectiveness of gametic embryogenesis in guava, as reported previously in other fruit crops (Nowaczyk et al, 2016;Abdollahi and Rashidi, 2018). The lack of anther swelling in media M3-M9 could be attributed to the addition of GA3 in these media, which was missing in M1, M2, M10, and M11 media inducing swelling in anthers.…”

Section: Effect Of Cold Treatment and Genotype On Pollen Viabilitysupporting

confidence: 79%

Embryogenic responses in anther derived calli of white and pink flesh guava (Psidium guajava L.) cultivars

Bokhari

2023

PAKJAS

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Androgenesis is reported in a few tree crops such as citrus and papaya; however, there is little information available about guava. Different modified Chu (N6) media and cold pretreatments of floral buds were tested to induce embryogenic calli in the anthers of two white flesh guava cultivars, ‘Round’ and ‘Pyriform’. Four modified Chu (N6) media formulations, i.e., M1 (400 mgL-1 casein hydrolysate, 200 mgL-1 glutamine, 0.2 mgL-1NAA, 1.0 mgL-1 KIN, 0.5 mgL-1 6-BAP, 0.5 mgL-1 zeatin and 5% coconut water), M2 (400 mgL-1 casein hydrolysate, 200 mgL-1 glutamine, 0.2 mgL-1NAA, 1.0 mgL-1 KIN, 0.5 mgL-1 6-BAP), M10 (0.5 mgL-1 2,4-D), and M11 (0.5 mgL-1 2,4-D and 50 mgL-1 PVP) enhanced anther swelling and callus formation. Genotypic variability was found in pollen viability, phenolic exudation, and anther tissue browning after cold pretreatment for 0-72 hrs. intervals. Anthers of ‘Round’ showed more pollen viability across cold pretreatment intervals with less phenolic exudations and tissue browning than ‘Pyriform’. On both M10 and M11 media, anthers with uni-nucleate microspores developed embryogenic calli; however, anthers of ‘Pyriform’ resulted in more calli induction (69.58%) on M10 media, whereas anthers of ‘Round’ developed more calli (66.60%) on M11 media. The proliferating calli in white flesh ‘Round’ and ‘Pyriform’ cultivars and pink flesh ‘Round’ cultivar formed more embryogenic masses upon transfer to growth hormone-free Chu (N6) media under light conditions; however, embryos showed delayed maturity with limited germination. More genotypes shall be explored to enhance embryo germination, and embryogenic media shall be further fine-tuned. This study provides the foundation for the generation of homozygous lines in guava using androgenesis

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“…Por su parte, Ercan y Ayar (2011) reportaron resultados similares los cuales variaron de 0 a 7.69% de formación de embriones; sin embargo, únicamente obtuvieron 12 plantas completas. El efecto de edad de la planta donante del explante (Kristiansen y Andersen, 1993), las condiciones de crecimiento (Büyükalaca et al, 2004), la temporada de crecimiento (Ata et al, 2019), los reguladores de crecimiento (Nowaczyk et al, 2016) y el genotipo de la planta donante afectan la eficiencia y éxito de la embriogénesis y ulterior desarrollo a planta completa; sin embargo, es el genotipo el factor principal que define la embriogénesis exitosa de microsporas a partir del cultivo de anteras (Niklas et al, 2012;Keles et al, 2015). Uno de los principales objetivos de mejoramiento en chile de tipo mulato y ancho en el Programa de Mejoramiento de Chile en el Campo Experimental Bajío del INIFAP es obtener líneas con resistencia a Phytophthora capsici Leo.…”

Section: Introductionunclassified

Inducción de embriones androgénicos y regeneración de plantas haploides en genotipos experimentales de chile poblano a través de cultivo de anteras

González-Chavira

Guerrero-Aguilar

Pons-Hernández

et al. 2023

Remexca

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Los métodos tradicionales para obtener líneas puras u homocigotas involucran varias generaciones de autofecundación con un requerimiento importante de tiempo. La obtención de plantas haploides y doble haploides, representa una alternativa que reduce a una generación, la producción de líneas con alelos idénticos en todos sus cromosomas. El objetivo de este trabajo fue implementar el protocolo de obtención de embriones androgénicos en poblaciones de chile mulato y ancho del programa de mejoramiento de chile en el Campo Experimental Bajío del Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias durante 2019 y 2020. Treinta genotipos de chile comprendidos en dos poblaciones. a) población criolla heterogénea de chiles mulato y ancho con característica de esterilidad masculina citoplasmática; y b) población de cruzas interespecíficas de chile ancho con chile habanero (C. annuum x C. chinense) resistentes a geminivirus (PepGMV) y Phytophthora sp., fueron sometidas a tratamiento de inducción androgénica. El tratamiento consistió en N 6 -furfuriladenina (cinetina) (0.01 mg L-1) y ácido 2,4-diclorofenoxiacético (2,4-D) (0.01 mg L-1) para la inducción de embriogénesis somática y posteriormente de cinetina (0.1 mg L-1) para la inducción de germinación de embriones. La duplicación cromosómica se realizó por medio de colchicina (0.5%) previo a su adaptación in vivo. La población conformada por genotipos derivados de cruzas interespecíficas obtuvo mayor eficiencia de plantas regeneradas doble haploides entre 4.29% y 14.67%, mientras que la población criolla generó embriones en menor proporción entre 1.02% y 5.26%. Estos resultados son los primeros reportados para obtención de plantas doble haploides de chiles mulato y ancho.

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Treating donor plants with 2,4-dichlorophenoxyacetic acid can increase the effectiveness of induced androgenesis in Capsicum spp.

Cited by 10 publications

References 18 publications

Creation of pepper doubled haploids and morphological characterization of androgenic plants

Creation of pepper doubled haploids and morphological characterization of androgenic plants

Embryogenic responses in anther derived calli of white and pink flesh guava (Psidium guajava L.) cultivars

Inducción de embriones androgénicos y regeneración de plantas haploides en genotipos experimentales de chile poblano a través de cultivo de anteras

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