Treat cancers by targeting survivin: Just a dream or future reality?

Coumar, Mohane Selvaraj; Tsai, Fang Ying; Kanwar, Jagat R.; Sarvagalla, Sailu; Cheung, Chun Hei Antonio

doi:10.1016/j.ctrv.2013.02.002

Cited by 133 publications

(145 citation statements)

References 91 publications

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“…In contrast to HT-29 cells, the protein content increased after 24 h incubation in Caco-2 cells except in the 0.15 µM HYP group, which indicated higher cell number and lower cytotoxicity in these groups ( Figure 1B). Survivin (BIRC5) is a member of the IAPs family that is expressed in many different cancer cell types (Coumar et al, 2013). Survivin contributes several processes related to the regulation of mitosis, apoptosis, and cellular stress response, but it cannot inhibit caspases directly (Mikešová et al, 2013).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of apoptotic cell death mechanisms induced by hypericin-mediated photodynamic therapy in colon cancer cells

Süloğlu¹,

Karacaoğlu²,

Selmanoğlu³

et al. 2016

Turk J Biol

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Hypericin (HYP)-mediated photodynamic therapy (PDT) is a new alternative treatment strategy for colon cancer inducing various cell-death pathways. Apoptotic cell death is the desired cellular fate in cancer cells. Therefore, we investigated the apoptotic pathways by determining apoptosis-related proteins (survivin, caspase-3, caspase-9, Bcl-2, and Bax) at the mRNA level using real-time polymerase chain reaction (qPCR), and the percentage of survivin was determined by survivin ELISA in HT-29 and Caco-2 colon cancer cell lines. The downregulation of survivin was significant 16 h after PDT for both cells, while caspase-3 upregulation was apparent 24 h after PDT. Caspase-9 and caspase-3 upregulations were parallel to each other. There was no Bcl-2 expression in HT-29 cells, but we observed downregulation in Bcl-2 expression in Caco-2 cells after HYP photoactivation. The Bax expression downregulated significantly after 24 h incubation in HT-29 cells, while it was upregulated after 16 h incubation in Caco-2 cells. The present study provides evidence that HYP-induced alterations in apoptosis-related protein expression ended in different responses in HT-29 and Caco-2 colon cancer cells, and these may be used in the treatment of chemotherapy-resistant cancer types.

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Section: Resultsmentioning

confidence: 99%

Evaluation of apoptotic cell death mechanisms induced by hypericin-mediated photodynamic therapy in colon cancer cells

Süloğlu¹,

Karacaoğlu²,

Selmanoğlu³

et al. 2016

Turk J Biol

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“…Unlike other IAP members, survivin is largely expressed in embryonic and fetal tissues as well as in many malignant cells, but not in normal adult tissues (Zaffaroni and Daidone, 2002;Coumar et al, 2013). Previous reports have demonstrated that the overexpression of survivin is correlated with tumor progression, poor prognosis and drug resistance in many cancers including AML (Adida et al, 2000;Fukuda and Pelus, 2006).…”

Section: Sirna-mediated Silencing Of Survivin Inhibits Proliferationmentioning

confidence: 99%

siRNA-mediated Silencing of Survivin Inhibits Proliferation and Enhances Etoposide Chemosensitivity in Acute Myeloid Leukemia Cells

Karami¹,

Baradaran²,

Esfahani³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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Background: Overexpression of survivin, a known inhibitor of apoptosis, is associated with tumor progression and drug resistance in numerous malignancies, including leukemias. The aim of this study was to investigate the effect of a specific survivin small interference RNA (siRNA) on proliferation and the sensitivity of HL-60 acute myeloid leukemia (AML) cells to the chemotherapeutic drug etoposide. Materials and Methods: The cells were transfected with siRNAs using Lipofectamine™2000 transfection reagent. Relative survivin mRNA and protein levels were measured by quantitative real-time PCR and Western blotting, respectively. Trypan blue exclusion assays were performed to monitor tumor cell proliferation after siRNA transfection. The cytotoxic effects of etoposide and survivin siRNA, alone and in combination, on leukemic cells were determined using MTT assay. Apoptosis was assessed by ELISA cell death assay. Results: Survivin siRNA markedly reduced both mRNA and protein expression levels in a time-dependent manner, leading to distinct inhibition of cell proliferation and increased spontaneous apoptosis. Surprisingly, survivin siRNA synergistically increased the cell toxic effects of etoposide. Moreover, survivin down-regulation significantly enhanced its induction of apoptosis. Conclusions: Our study suggests that down-regulation of survivin by siRNA can trigger apoptosis and overcome drug resistance of leukemia cells. Therefore, survivin siRNA may be an effective adjuvant in AML chemotherapy

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“…AFP is widely used as a biomarker for HCC diagnosis, monitoring, and follow-up, but studies of its biological function have only recently increased. Numerous studies confirmed that AFP has complicated biological functions, such as promoting liver cancer cell proliferation, inhibition of apoptosis, and immune evasion (Li et al, 2009;Coumar et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…It shows positive expression in the vast majority of tumor tissues. These unique expression characteristics make it a target for cancer diagnosis and therapy (Rödel et al, 2012;Coumar et al, 2013). Studies have shown that Survivin was highly expressed in hepatoma cells and that it can regulate cell division, proliferation, and apoptosis (Zhu et al, 2005;Zhao et al, 2011;Liu et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Effects of AFP gene silencing on Survivin mRNA expression inhibition in HepG2 cells

Fang

Han

et al. 2015

Genet. Mol. Res.

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ABSTRACT. We investigated the effects of alpha-fetoprotein (AFP) gene silencing on Survivin expression in HepG2 cells. Small interfering RNA technology was used to downregulate AFP expression in HepG2 cells. An enzyme-linked immunosorbent assay was used to measure AFP concentration in the supernatant before and after transfection. An MTT assay was used to detect cell proliferation activity before and after transfection. We performed flow cytometric analysis to detect the cell apoptosis rate, and reverse transcription-polymerase chain reaction to detect Survivin mRNA levels before and after transfection. Fortyeight hours after transfection, AFP concentration in the supernatant of the experimental group significantly decreased, hepatocellular carcinoma cell growth was inhibited by 43.1%, and the apoptosis rate increased by 24.3%. Survivin mRNA expression was reduced by 78.0% in HepG2 cells. These indicators in the control group and in the 3185 Effects of AFP gene silencing on Survivin ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (2): 3184-3190 (2015) blank group did not change significantly. Silencing of AFP expression in HepG2 cells can effectively inhibit the growth of hepatoma cells and promote apoptosis, which may be useful for reducing intracellular Survivin mRNA levels.

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Treat cancers by targeting survivin: Just a dream or future reality?

Cited by 133 publications

References 91 publications

Evaluation of apoptotic cell death mechanisms induced by hypericin-mediated photodynamic therapy in colon cancer cells

Evaluation of apoptotic cell death mechanisms induced by hypericin-mediated photodynamic therapy in colon cancer cells

siRNA-mediated Silencing of Survivin Inhibits Proliferation and Enhances Etoposide Chemosensitivity in Acute Myeloid Leukemia Cells

Effects of AFP gene silencing on Survivin mRNA expression inhibition in HepG2 cells

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