Kukundara Marma, a Vaikalyakara Marma structurally categorized under Sandhi Marma. The location is said as on either side of 'Pristavamsha' on 'Jagana Bahirbaghaga',injury to which causes loss of movement and loss of sensation. Regarding its location and identificatio n there is lot of discrepancy among the authors in interpreting particular structure. Hence there is a need to identify specific anatomical structure, its location and regional involvement to ascertain structural composition of Kukundara Marma and to evaluate its involvement in injury. The study was conducted in three steps as literary study, Regional anatomical study by dissection and further retrospective clinical observational study of thirty patients for confirmation of location and impact of Kukundara Marma injury. The data subjected for statistical evaluation showed that male & female accounted in this study was 53.3%, 46.7% respectively, while age group was 31-50, 60% Amongst the causes of injuries, trauma at lumbosacral region was 80%. Pain referral zone was more in leg region 43.3%, Chestahani found in 93% cases. Gait was abnormal in 93%. Examination wa s positive in 53.3% It was concluded that Cadaveric observation has favored in determining the Kukundara Marma as slightly movable complex sacroiliac joint. The exact location of Kukundara Marma marked over an area was present on the side of vertebral column & outer aspect of Gluteal region is substantiated by dissection and the soft tissue component predominantly involved was neurovascular structure which was present in close association with lower part of sacroiliac joint. Vaikalyakaratva of Kukundara Marma has confirmed by observing the signs and symptoms of 30 cases who had injury to low back or gluteal region.