Traumatic resin ducts induced by methyl jasmonate in Pinus spp

López-Villamor, Adrián; Zas, Rafael; Pérez, A.; Escudero, Yonatan Cáceres; Silva, Marta Nunes da; Vasconcelos, Marta W.; Vázquez‐González, Carla; Sampedro, Luís; Solla, Alejandro

doi:10.1007/s00468-020-02057-9

Cited by 18 publications

(9 citation statements)

References 64 publications

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“…From a plant protection perspective, exogenous application of phytohormones such as jasmonic acid and its methyl ester (methyl jasmonate) has been proposed as a tool to prime or induce defenses associated with conifer resistance to insects and fungal pests ( Holopainen et al, 2009 ; Zas et al, 2014 ; Mageroy et al, 2020a ). Treatment with methyl jasmonate (MeJA), for example, induces the formation of traumatic resin ducts and synthesis of terpene and phenolic-based compounds ( Krokene et al, 2008 ; López-Villamor et al, 2020 ). These MeJA-mediated changes have been shown to increase resistance against bark-feeding insect pests like the pine weevil Hylobius abietis ( Heijari et al, 2005 ; Zas et al, 2014 ; Fedderwitz et al, 2016 ; Chen Y. et al, 2020 ), and the spruce bark beetle-blue stain fungus complex Ips typographus - Endoconidiophora polonica ( Zhao et al, 2011 ; Schiebe et al, 2012 ).…”

Section: Introductionmentioning

confidence: 99%

Variation in Methyl Jasmonate-Induced Defense Among Norway Spruce Clones and Trade-Offs in Resistance Against a Fungal and an Insect Pest

et al. 2021

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An essential component of plant defense is the change that occurs from a constitutive to an induced state following damage or infection. Exogenous application of the plant hormone methyl jasmonate (MeJA) has shown great potential to be used as a defense inducer prior to pest exposure, and could be used as a plant protection measure. Here, we examined (1) the importance of MeJA-mediated induction for Norway spruce (Picea abies) resistance against damage by the pine weevil Hylobius abietis, which poses a threat to seedling survival, and infection by the spruce bark beetle-associated blue-stain fungus Endoconidiophora polonica, (2) genotypic variation in MeJA-induced defense (terpene chemistry), and (3) correlations among resistance to each pest. In a semi-field experiment, we exposed rooted-cuttings from nine different Norway spruce clones to insect damage and fungal infection separately. Plants were treated with 0, 25, or 50 mM MeJA, and planted in blocks where only pine weevils were released, or in a separate block in which plants were fungus-inoculated or not (control group). As measures of resistance, stem area debarked and fungal lesion lengths were assessed, and as a measure of defensive capacity, terpene chemistry was examined. We found that MeJA treatment increased resistance to H. abietis and E. polonica, but effects varied with clone. Norway spruce clones that exhibited high constitutive resistance did not show large changes in area debarked or lesion length when MeJA-treated, and vice versa. Moreover, insect damage negatively correlated with fungal infection. Clones receiving little pine weevil damage experienced larger lesion lengths, and vice versa, both in the constitutive and induced states. Changes in absolute terpene concentrations occurred with MeJA treatment (but not on proportional terpene concentrations), however, variation in chemistry was mostly explained by differences between clones. We conclude that MeJA can enhance protection against H. abietis and E. polonica, but the extent of protection will depend on the importance of constitutive and induced resistance for the Norway spruce clone in question. Trade-offs among resistances do not necessarily hinder the use of MeJA, as clones that are constitutively more resistant to either pest, should show greater MeJA-induced resistance against the other.

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Section: Introductionmentioning

confidence: 99%

Variation in Methyl Jasmonate-Induced Defense Among Norway Spruce Clones and Trade-Offs in Resistance Against a Fungal and an Insect Pest

et al. 2021

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“…In order to differentiate traumatic resin ducts from constitutive resin ducts, we adopted the criteria that TDs generally form a tangential band (Panshin and de Zeeuw, 1980; Figure 1 Location of the field progeny trial (yellow star) and mother-trees selected as phenotypically superior in resin yield (yellow circles) of Pinus oocarpa in the municipality of Ario, state Michoacán, Mexico. Vázquez-González et al, 2020;López-Villamor et al, 2021). That means that more than two canals are side by side, forming a protective barrier.…”

Section: Selection and Collection Of Samples For Anatomical Studiesmentioning

confidence: 99%

“…Due to its high toxicity, it is stored under pressure in specialized structures called resin ducts, which are multicellular structures surrounded by layers of epithelial cells (Lewinsohn et al, 1991). Its formation may be a constitutive feature of development in plant tissues and organs (normal axial and radial ducts) or induced by external factors (traumatic ducts) (Pearce, 1996;da Silva Rodrigues-Corrêa et al, 2013;Vázquez-González et al, 2020;López-Villamor et al, 2021). The traumatic ducts (TDs) in the wood of conifers can be radial (longitudinal) or axial (transverse), and unlike typical ducts, they can be found in all (Lin et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…The formation of TDs is believed to increase resin yield and flow (Ruel et al, 1998;Lombardero et al, 2000) in response to injuries caused by frost, mechanical, chemical, pest or pathogenic damage (Cabrita, 2021). In Pinus, in general, inducibility of resin ducts and the TD traits in pine trees have been studied very little (seven species) (López-Villamor et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Traumatic ducts size varies genetically and is positively associated to resin yield of Pinus oocarpa open-pollinated progenies

et al. 2022

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Mexico ranks 5th in worldwide resin production. Pinus oocarpa is the most widely tapped pine tree in Mexico. Michoacán (central-western Mexico) is the first nationwide state producer of resin. Despite the P. oocarpa relevance, there is no genetic improvement program in the country for resin production. We evaluated the degree of genetic control for growth, anatomical traits, resin yield, and the correlation among them at an early age (five-years-old for growth, six for resin, and anatomical traits) in a P. oocarpa half-sib progeny trial. Families were originated from selected mother trees, based on their resin yield. We found significant genetic variation among families for stem volume (h 2 i = 0.12, h 2 f = 0.35), traumatic ducts (diameter: h 2 i = 0.63, h 2 f = 0.51; area: h 2 f = 0.81), and resin yield (individual and family narrow-sense heritability: h 2 i = 0.20, h 2 f = 0.52), and a positive correlation between diameter and area of traumatic ducts with resin yield (Pearson correlation: r= 0.73, p= 0.04; r= 0.71, p= 0.0497; respectively). Results suggest that the early selection (six-years-old) of superior P. oocarpa families, based on resin yield (estimated by microchipping technique), and/or based on larger diameter and area of traumatic ducts, appears to be a feasible strategy to develop seed orchards able to provide genetically improved seeds for intensive resin tree plantations. That would be an important alternative for a state as Michoacán, Mexico, where natural P. oocarpa stands are being replaced for avocado orchards for exportation.

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“…Attacking pests/pathogens are initially confronted with constitutive defenses that may include both anatomical and biochemical barriers, such as the epidermis or outer bark, and constitutive antimicrobial compounds, such as terpenoids (the major constituents of resin) and soluble and cell wall‐bound phenolics and their derivatives (Krokene, 2015). This local response is further accompanied by an inducible defense response, which may involve physiological or anatomical changes, such as stomatal closure (Du et al, 2014), necrophylactic periderm formation (Morris et al, 2020), traumatic resin duct formation (López‐Villamor et al, 2021; Luchi et al, 2005), and phytoalexin‐type responses involving specialized metabolites like phenolics, lignin, and terpenoids (Franceschi et al, 2005; Van Bel & Gaupels, 2004).…”

Section: Introductionmentioning

confidence: 99%

Terpenoids are involved in the expression of systemic‐induced resistance in Austrian pine

Ghosh,

Ishangulyyeva,

Erbilgin

et al. 2024

Plant Cell & Environment

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Terpenoids are defense metabolites that are induced upon infection or wounding. However, their role in systemic‐induced resistance (SIR) is not known. Here, we explored the role of terpenoids in this phenomenon at a very early stage in the interaction between Austrian pine and the tip blight and canker pathogen Diplodia pinea. We induced Austrian pine saplings by either wounding or inoculating the lower stems with D. pinea. The seedlings were then challenged after 12 h, 72 h, or 10 days with D. pinea on the stem 15 cm above the induction. Lesion lengths and terpenoids were quantified at both induction and challenge locations. Key terpenoids were assayed for antifungal activity in in vitro bioassays. SIR increased with time and was correlated with the inducibility of several compounds. α‐Pinene and a cluster of β‐pinene, limonene, benzaldehyde, dodecanol, and n‐dodecyl acrylate were positively correlated with SIR and were fungistatic in vitro, while other compounds were negatively correlated with SIR and appeared to serve as a carbon source for D. pinea. This study shows that, overall, terpenoids are involved in SIR in this system, but their role is nuanced, depending on the type of induction and time of incubation. We hypothesize that some, such as α‐pinene, could serve in SIR signaling.

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Traumatic resin ducts induced by methyl jasmonate in Pinus spp

Cited by 18 publications

References 64 publications

Variation in Methyl Jasmonate-Induced Defense Among Norway Spruce Clones and Trade-Offs in Resistance Against a Fungal and an Insect Pest

Variation in Methyl Jasmonate-Induced Defense Among Norway Spruce Clones and Trade-Offs in Resistance Against a Fungal and an Insect Pest

Traumatic ducts size varies genetically and is positively associated to resin yield of Pinus oocarpa open-pollinated progenies

Terpenoids are involved in the expression of systemic‐induced resistance in Austrian pine

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