Traumatic brain injury in piglets of different ages: techniques for lesion analysis using histology and magnetic resonance imaging

Grate, Loretta L.; Golden, Jeffrey A.; Hoopes, P. Jack; Hunter, Jill V.; Duhaime, Ann Christine

doi:10.1016/s0165-0270(02)00361-8

Cited by 44 publications

(37 citation statements)

References 16 publications

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“…Immunohistochemistry enabled the authors to propose a hypothesis of functional brain maturation to explain the effect of age on brain activation measured by fMRI (Fang et al 2005a). It has also been demonstrated that the volumetric analysis of brain lesions by MRI reveals the impact of traumatic brain injury in a similar way to histological approaches (Grate et al 2003; Fig. 5).…”

Section: Brain Injurymentioning

confidence: 95%

“…Serial T2-weighted MR images, histological section stained with hematoxylin and eosin, and adjacent section stained with an antibody against glial fibrillary acidic protein obtained at one-month post-injury in a one-month old piglet subjected to scaled focal brain injury. Note that the traumatic brain lesion (green arrow) is found whatever the method (adapted from Grate et al 2003). …”

Section: Brain Injurymentioning

confidence: 99%

See 1 more Smart Citation

MRI Techniques and New Animal Models for Imaging the Brain

Chaillou¹,

Tillet²,

Andersson³

2012

When Things Go Wrong - Diseases and Disorders of the Human Brain

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Section: Brain Injurymentioning

confidence: 95%

Section: Brain Injurymentioning

confidence: 99%

MRI Techniques and New Animal Models for Imaging the Brain

Chaillou¹,

Tillet²,

Andersson³

2012

When Things Go Wrong - Diseases and Disorders of the Human Brain

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“…The pig's brain, being relatively large, is suitable for imaging techniques and machines used for humans, for instance, magnetic resonance imaging, computed tomography, single photon emission computed tomography (SPECT) or positron emission tomography (PET; Figure 1). Thus, pigs have been used as a model for human research in a wide range of imaging studies, such as in traumatic brain injury (Grate et al, 2003), Parkinson's disease (Mikkelsen et al, 1999;Cumming et al, 2003) or stroke (Sakoh et al, 2000;Røhl et al, 2002). Anatomical brain imaging studies on pigs have allowed the identification of swine cerebral structures and the conception of stereotaxic atlases of the pig brain (e.g.…”

Section: Neurobiological Similaritiesmentioning

confidence: 99%

Food preferences and aversions in human health and nutrition: how can pigs help the biomedical research?

Clouard

Meunier-Salaün

Val‐Laillet

2012

Animal

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The establishment of food preferences and aversions determines the modulation of eating behaviour and the optimization of food intake. These phenomena rely on the learning and memory abilities of the organism and depend on different psychobiological mechanisms such as associative conditionings and sociocultural influences. After summarizing the various behavioural and environmental determinants of the establishment of food preferences and aversions, this paper describes several issues encountered in human nutrition when preferences and aversions become detrimental to health: development of eating disorders and obesity, aversions and anorexia in chemotherapy-treated or elderly patients and poor palatability of medical substances and drugs. Most of the relevant biomedical research has been performed in rodent models, although this approach has severe limitations, especially in the nutritional field. Consequently, the final aim of this paper is to discuss the use of the pig model to investigate the behavioural and neurophysiological mechanisms underlying the establishment of food preferences and aversions by reviewing the literature supporting analogies at multiple levels (general physiology and anatomy, sensory sensitivity, digestive function, cognitive abilities, brain features) between pigs and humans.Keywords: pig, conditioned learning, eating behaviour, animal model, biomedical applications ImplicationsInvestigation of the behavioural and neurophysiological mechanisms of the establishment of food preferences and aversions can lead to important developments in the context of human nutrition and health. Because the rodent models are not always adequate in this field, there is a need to develop alternative experimental models. Pigs have numerous similarities with humans in terms of the physiology, anatomy, sensory sensitivity, cognitive abilities and brain functions. The aim of this paper is to promote the use of pigs for biomedical research in human nutrition. IntroductionFeeding is a complex behaviour, which can be described as 'the research and consumption of food and drink to maintain vital functions ' (Bellisle, 1999) and to 'fulfil the metabolic needs of the organism ' (Ferreira, 2004). Today, it is also well acknowledged that a high proportion of human food consumption in developed countries appears to be driven by pleasure (for a review, see Lowe and Butryn, 2007) and sociocultural influences. Food consumption is also involved in fundamental metabolic homeostasis regulation, as it controls the supply of energy and nutrients in the organism (Bellisle, 1999). According to Ferreira (2004), feeding behaviour implies that animals learn to consume high-energy foods and to avoid toxic foods. Establishment of food selection implies that, during its first experience with food, the organism memorizes the sensorial characteristics of the food (e.g. taste, odour, texture and visual cues) and the postingestive consequences of its ingestion, and associates these food characteristics with these consequences (Garcia et ...

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“…In the last decade, an increasing number of studies in the field of neuroscience has been reported (Lind et al, 2007). In addition to the study of the action or metabolism of various pharmacological drugs or neurotransmitters (Bhalla et al, 2002;Rosa-Neto et al, 2004a;Lind et al, 2005b;Minuzzi et al, 2005), pathological models dealing with traumatic brain injury (Grate et al, 2003), stroke (Sakoh et al, 2001) or neurodegenerative diseases such as Parkinsonism induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine hydrochloride (MPTP) poisoning (Danielsen et al, 2000;Cumming et al, 2001; Dall et al, 2002) have also been developed.The major benefit of the swine for neuroscience research remains the size of its brain. It is large enough to allow evoked potential (EP) recordings, neurosurgery and conventional imaging in living animals.…”

mentioning

confidence: 99%

The pig model in brain imaging and neurosurgery

Sauleau

Lapouble

Val‐Laillet

et al. 2009

Animal

207

178

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The pig model is increasingly used in the field of neuroscience because of the similarities of its brain with human. This review presents the peculiarities of the anatomy and functions of the pig brain with specific reference to its human counterpart. We propose an approximate mapping of the pig's cortical areas since a comprehensive description of the equivalent of Brodmann's areas is lacking. On the contrary, deep brain structures are received more consideration but a true three-dimensional (3D) atlas is still eagerly required. In the second section, we present an overview of former works describing the use of functional imaging and neuronavigation in the pig model. Recently, the pig has been increasingly used for molecular imaging studies using positron emission tomography (PET). Indeed, the large size of its brain is compatible with the limited spatial resolution of the PET scanner built to accommodate a human being. Similarly, neuronavigation is an absolute requirement to target deep brain areas in human and in pig since the surgeon cannot rely on external skull structures for zeroing the 3D reference frame. Therefore, a large body of methodological refinements has been dedicated to image guided surgery in the pig model. These refinements allow now a millimetre precision: an absolute requirement for basal nuclei targeting. In the third section, several examples of ongoing studies in our laboratory were presented to illustrate the intricacies of using the pig model. For both examples, after a brief description of the scientific context of the experiment, we present, in detail, the methodological steps required to achieve the experimental goals, which are specific to the porcine model. Finally, in the fourth section, the anatomical variations depending on the breed and age are discussed in relation with neuronavigation and brain surgery. The need for a digitized multimodality brain atlas is also highlighted.Keywords: pig, neuroimaging, single photon emission tomography, neuronavigation, deep brain stimulation ImplicationsThe pig model is increasingly used in the field of neuroscience because of the similarities of its brain with human. Indeed, aside from the rodent model there is a critical need for a large animal model ethically acceptable, i.e. excluding non-human primates. In this review we present some experiments dealing with the various procedures achievable in the pig model ranging from image-guided brain surgery to functional brain imaging studies. The recent advances in functional imaging data processing pointed out our partial knowledge of pig brain neuro-anatomy and the requirement for a digitalized atlas matching MRI (magnetic resonance imaging) and histological resources. IntroductionSwine have been used extensively as a model of human in biomedical researches such as cardiovascular, metabolic and transplantation (Phillips et al., 1982;Larsen and Rolin, 2004;Imai et al., 2006;Groth, 2007). In the last decade, an increasing number of studies in the field of neuroscience has been reported (Lind et ...

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Traumatic brain injury in piglets of different ages: techniques for lesion analysis using histology and magnetic resonance imaging

Cited by 44 publications

References 16 publications

MRI Techniques and New Animal Models for Imaging the Brain

MRI Techniques and New Animal Models for Imaging the Brain

Food preferences and aversions in human health and nutrition: how can pigs help the biomedical research?

The pig model in brain imaging and neurosurgery

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