TraT lipoprotein, a plasmid-specified mediator of interactions between gram-negative bacteria and their environment.

Sukupolvi, Soila; O’Connor, C. David

doi:10.1128/mmbr.54.4.331-341.1990

Cited by 41 publications

(23 citation statements)

References 65 publications

(99 reference statements)

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“…Some plasmid-encoded transfer functions may themselves play a role in bacterial virulence. For example, the TraT protein is a cell-surface-exposed, outer membrane lipoprotein specified by large, usually conjugative, Flike plasmids in Gram-negative bacteria that resists the bactericidal activities of serum (67).…”

Section: Antibiotic Resistance and Virulence Associated With Plasmidsmentioning

confidence: 99%

Bacterial plasmids in the oral and endodontic microflora

Sedgley¹,

Clewell²

2004

Endodontic Topics

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Bacterial plasmids typically represent covalently closed circular, double‐stranded, supercoiled DNA molecules that replicate independently of chromosomal DNA. Plasmids are frequently not essential to the bacterial host, but can confer traits facilitating survival under atypical conditions e.g. resistance to antibiotics and heavy metals. Plasmids have been identified in clinical and environmental bacteria from all over the world, and range in size from ∼1 to >200 kb. Their copy number in the cell can range from one or two copies per chromosome, to well over 30; the number is usually characteristic for the particular plasmid. Clinical strains of Enterococcus faecalis, a species commonly associated with root canal infections, can carry as many as six co‐resident plasmids with different sizes and copy numbers. In part because of their ability to replicate autonomously, plasmids are used as tools or shuttle vectors in basic molecular biological research. Many plasmids can transfer copies of themselves from one bacterial cell to another by a process called conjugation. In some cases, small peptides called pheromones act as mating signals in this process. Conjugative plasmids that make use of pheromones have been identified previously in E. faecalis and the related plasmids tend to exhibit a narrow host range. Conjugative plasmids in E. faecalis that do not make use of pheromones usually have a much broader host range. Pheromone‐responding plasmids in E. faecalis can carry genes relating to antibiotic resistance as well as virulence traits. In general, plasmids are capable of adaptability and stable maintenance in a wide spectrum of bacterial hosts. The potential role of plasmids in oral and endodontic microbiology is discussed.

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Section: Antibiotic Resistance and Virulence Associated With Plasmidsmentioning

confidence: 99%

Bacterial plasmids in the oral and endodontic microflora

Sedgley¹,

Clewell²

2004

Endodontic Topics

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“…The sequence of the F surface exclusion genes, traS (nt 22252 to 22698) and traT (nt 22796 to 23527), were determined by Jalajakumari et al (123); the sequence of the R100 traT gene was determined by Ogata et al (197); and the sequence of the ColB-K98 TraT protein was reported by Sukupolvi and O'Connor (241). The sequences of traT for F and pED208 were compared by Finlay and Paranchych (66), and the known sequences of TraT were compared by Finlay and Paranchych (66), Jalajakumari et al (123), and Sukupolvi and O'Connor (241).…”

Section: Surface Exclusionmentioning

confidence: 99%

Analysis of the sequence and gene products of the transfer region of the F sex factor.

Frost

Ippen‐Ihler

Skurray

1994

Microbiological Reviews

288

274

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within this region are marked as a double-dashed line. Signal sequence cleavage sites are indicated by vertical arrows below the line. Features of interest, including homology to signature sequences mentioned in the legends to Fig. 3 and 4, are underlined and described below the line. The nick site (A, nt 140 to 141 according to reference 173), the site of the IS3 insertion, and the positions of frameshift mutations (V) are indicated below and above the line, respectively. The restriction sites for common restriction endonucleases are given in boldface type above the line, and the nucleotide on the 5' side of the cut site is also in boldface type. Nucleotides in parentheses above the line in the traX-finO region (nt 32466 to end) represent the sequence of the F plasmid as reported by Yoshioka et al. (274). MICROBIOL. REV.

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“…Surface exclusion reduces redundant conjugation between cells carrying similar transfer systems. In F-like plasmids, two gene products are responsible for surface exclusion, TraS and TraT, which act through distinct mechanisms (reviewed in Frost et ai, 1994;Sukupolvi and O'Connor, 1990). Four different allelic groups of TraT have been idenfified based on its specificity of action.…”

Section: Riapl Rfapmentioning

confidence: 99%

“…The member plasmids of each surface exclusion group almost always share the same conjugative pilus type (Willetts and Maule, 1985), suggesting that the pilus and TraT protein may interact. The sequences of the IncF TraT proteins are almost identical (Finlay and Paranchych, 1986;Sukupolvi and O'Connor, 1990;Harrison et ai, 1992), but these minor differences have been correlated with the specificity of TraT action (Harrison et ai, 1992). TraT is an oligomeric lipoprotein of the outer membrane that reduces transfer 10-30-fold (Perumal and Minkley, 1984).…”

Section: Riapl Rfapmentioning

confidence: 99%

The role of the pilus in recipient cell recognition during bacterial conjugation mediated by F‐like plasmids

Anthony

Sherburne

et al. 1994

Molecular Microbiology

100

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The effects of defined mutations in the lipopolysaccharide (LPS) and the outer membrane protein OmpA of the recipient cell on mating-pair formation in liquid media by the transfer systems of the F-like plasmids pOX38 (F), ColB2 and R100-1 were investigated. Transfer of all three plasmids was affected differently by mutations in the rfa (LPS) locus of the recipient cell, the F plasmid being most sensitive to mutations that affected rfaP gene expression which is responsible for the addition of pyrophosphorylethanolamine (PPEA) to heptose I of the inner core of the LPS. ColB2 transfer was more strongly affected by mutations in the heptose II-heptose III region of the LPS (rfaF) whereas R100-1 was not strongly affected by any of the rfa mutations tested. ompA but not rfa mutations further decreased the mating efficiency of an F plasmid carrying a mutation in the mating-pair stabilization protein TraN. An F derivative with a chloramphenicol acetyltransferase (CAT) cassette interrupting the traA pilin gene was constructed and pilin genes from F-like plasmids (F, ColB2, R100-1) were used to complement this mutation. Unexpectedly, the results suggested that the differences in the pilin sequences were not responsible for recognizing specific groups in the LPS, OmpA or the TraT surface exclusion protein. Other corroborating evidence is presented suggesting the presence of an adhesin at the F pilus tip.

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TraT lipoprotein, a plasmid-specified mediator of interactions between gram-negative bacteria and their environment.

Cited by 41 publications

References 65 publications

Bacterial plasmids in the oral and endodontic microflora

Bacterial plasmids in the oral and endodontic microflora

Analysis of the sequence and gene products of the transfer region of the F sex factor.

The role of the pilus in recipient cell recognition during bacterial conjugation mediated by F‐like plasmids

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