traT and CNF2 genes of Escherichia coli isolated from milk of healthy cows and sheep

Açık, Mehmet Nuri; Yurdakul, N.E.; Çakici, L.; Onat, N.; Doğan, Özlem; Çetinkaya, Burhan

doi:10.1016/j.rvsc.2004.01.003

Cited by 7 publications

(3 citation statements)

References 18 publications

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“…Although, the rates of traT gene vary depending on the country, it is the most commonly obtained virulence factor in E. coli strains. Likewise, traT gene prevalence in previous studies varied from 36% to 62% [10,16]. In the current study, a higher rate (75%) was detected in the observed strains.…”

Section: Discussionsupporting

confidence: 41%

Detection of Several Virulence Properties, Antibiotic Resistance and Phylogenetic Relationship in E. Coli Isolates Originated from Cow Mastitis

2014

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Cow mastitis caused by Escherichia coli (E. coli) exhibits various local and systemic clinical signs at varying degrees of severity. The aim this study was to elucidate the virulence properties, antibiotic resistance and phylogenetics of 56 E. coli strains. Of all the studied strains, 12 were positive for hemolytic properties and 38 were positive for biofi lm production. Additionally, 55 of the strains were positive for multiple resistances in bacteriological tests. PCR analysis revealed that 42 strains carried the traT gene, 20 strains had the shiga toxin gene (stx1-stx2), and 8 strains carried the intimin gene (eae), but all strains were negative for aerobactin gene (aer). All strains encoding shiga toxin genes were also positive for stx1, but only 4 strains were positive for stx2. There were no signifi cant differences in virulence genes between antibiotic-resistant and antibiotic-susceptible strains. The random amplifi ed polymorphic DNA-polymerase chain reaction patterns revealed the existence of 13 main groups with 4 subgroups of E. coli. In this study, E. coli strains causing intramammary infections and originating from various sources might show resistance against common antibiotics. Pathogenity of E. coli that cause clinical mastitis, and prognosis of the infection might be predicted by obtaining the traT gene. Additionally, antibiotic resistance should be investigated at the genomic level to detect the relationship between virulence factors and antibiotic resistance. In fi eld conditions, development of antibiotic resistance is the main cause of mastitis treatment failure. Thus, antibiotic resistance profi les in herds should be monitored and effective antibiotics should be administered.

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Section: Discussionsupporting

confidence: 41%

Detection of Several Virulence Properties, Antibiotic Resistance and Phylogenetic Relationship in E. Coli Isolates Originated from Cow Mastitis

2014

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“…Nemeth et al (1991) identified the gene in 43 % of E. coli strains isolated from the milk of cows with mastitis. In another study by Acik et al (2004), milk samples from healthy cows and sheep were analysed and traT was present in 62.3 % of all isolates (62.5 % of the isolates from cows and 60 % of the isolates from sheep).…”

Section: Discussionmentioning

confidence: 99%

Comparison of virulence gene profiles of Escherichia coli isolates from sows with coliform mastitis and healthy sows

Gerjets

Traulsen

Reiners³

et al. 2011

Veterinary Microbiology

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Coliform mastitis (CM) is not only a serious economical and animal welfare touching problem in dairy cattle, but also in sows after farrowing. Due to this disease, the essential adequate supply with colostrum for the growth and the health of the piglets is not ensured. Besides other influencing factors, Escherichia (E.) coli is of great importance as a causative agent of this multifactorial disease. In this study, E. coli isolates from milk samples of healthy and CM-affected sows were examined for the presence of virulence genes associated with extraintestinal E. coli strains, enterotoxigenic E. coli and other pathogenic E. coli. The isolated E. coli harbored mainly virulence genes of extraintestinal E. coli strains (especially fimC, ompA, traT, hra, kpsMTII, iroN). The virulence gene spectrum for both samples from CM-affected and healthy sows did not differ significantly. Particular virulence gene profiles of E. coli isolates from diseased sows were not detected. This study provides novel insights into the role of E. coli in association with mastitis in sows since it is the first time E. coli isolates from CM-affected sows' milk were analysed for virulence genes. Because there were no differences in the prevalence of E. coli and their virulence-associated genes between healthy and diseased sows, other causative factors seem to have greater influence on the pathogenesis of porcine CM.

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“…It is considered the most important disease in dairy herds [1]. The most important causitave environmental mastitis pathogen is E.coli [2].…”

Section: Introductionmentioning

confidence: 99%

Molecular Studies on E.Coli Isolate from Milk of Mastatic Cattle with Special Reference to Associated Biochemical Changes in Kaliobea Governorate

2017

International Journal of Veterinary Sciences Research

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traT and CNF2 genes of Escherichia coli isolated from milk of healthy cows and sheep

Cited by 7 publications

References 18 publications

Detection of Several Virulence Properties, Antibiotic Resistance and Phylogenetic Relationship in E. Coli Isolates Originated from Cow Mastitis

Detection of Several Virulence Properties, Antibiotic Resistance and Phylogenetic Relationship in E. Coli Isolates Originated from Cow Mastitis

Comparison of virulence gene profiles of Escherichia coli isolates from sows with coliform mastitis and healthy sows

Molecular Studies on E.Coli Isolate from Milk of Mastatic Cattle with Special Reference to Associated Biochemical Changes in Kaliobea Governorate

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