Trash fish can be a source of betanodaviruses for cultured marine fish

Gomez, Dennis K.; Mori, Koichiro; Okinaka, Yasushi; Nakai, Toshiko; Park, Taesung

doi:10.1016/j.aquaculture.2010.02.033

Cited by 49 publications

(50 citation statements)

References 27 publications

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“…It can persist in the host for a long time sub-clinically and may cause severe mortality under extreme environmental conditions [105]. The horizontal transmission may occur from infected fish, feed, contaminated trash fish/carrier animals and contaminated water supply [18,44,47,69,87,119]. The cannibalistic nature of fish such as Asian seabass and brown-marbled grouper fingerlings may also enhance the horizontal transmission of the virus [78].…”

Section: Epidemiologymentioning

confidence: 99%

“…The cannibalistic nature of fish such as Asian seabass and brown-marbled grouper fingerlings may also enhance the horizontal transmission of the virus [78]. Feeding of trash fish to cultured fish is also found to be a source of infection [47]. Some of the betanodavirus infected larval fish can survive and act as a carrier for the next generation [87].…”

Section: Epidemiologymentioning

confidence: 99%

See 1 more Smart Citation

Betanodavirus of Marine and Freshwater Fish: Distribution, Genomic Organization, Diagnosis and Control Measures

et al. 2012

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Section: Epidemiologymentioning

confidence: 99%

Section: Epidemiologymentioning

confidence: 99%

Betanodavirus of Marine and Freshwater Fish: Distribution, Genomic Organization, Diagnosis and Control Measures

et al. 2012

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“…Based on phylogenetic analysis of the T4 variable region within the RNA2 segment, beta nodaviruses have been historically divided into 4 genotypes: striped jack nervous necrosis virus (SJNNV), tiger puffer nervous necrosis virus (TPNNV), barfin flounder nervous necrosis virus (BFNNV) and red-spotted grouper nervous necrosis virus (RGNNV) (Panzarin et al 2012). The phylo geny-based analyses of betanodaviruses previously reported in molluscs evidenced high homology with RGNNV (Gomez et al 2008a(Gomez et al , 2010 or RGNNV/ SJNNV reassortant strains (Panzarin et al 2012) circulating in fish in the areas of the studies. In our study, betanodaviruses were detected in skin, eyes and branchial-heart of both reared and wild common octopuses.…”

Section: Discussionmentioning

confidence: 99%

“…Betanodavirus infections have also been reported in marine invertebrates (Gomez et al 2008a,b, 2010, Panzarin et al 2012, including molluscs, such as the Mediterranean mussel Mytilus galloprovincialis Lamarck, 1819, the Japanese carpet shell Ruditapes philippinarum (Adams & Reeve, 1850) and the Japa nese common squid Todarodes pacificus (Steenstrup, 1880). Mediterranean mussels were found to be sub-clinically infected with betanodavirus, lo cated in the hepatopancreas (Gomez et al 2008a), while in Japanese common squid, betanodaviruses were detected in the brain and in eyes (Gomez et al 2008a(Gomez et al , 2010. Based on phylogenetic analysis of the T4 variable region within the RNA2 segment, beta nodaviruses have been historically divided into 4 genotypes: striped jack nervous necrosis virus (SJNNV), tiger puffer nervous necrosis virus (TPNNV), barfin flounder nervous necrosis virus (BFNNV) and red-spotted grouper nervous necrosis virus (RGNNV) (Panzarin et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Skin lesion-associated pathogens from Octopus vulgaris: first detection of Photobacterium swingsii, Lactococcus garvieae and betanodavirus

Fichi¹,

Cardeti²,

Perrucci³

et al. 2015

Dis. Aquat. Org.

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The common octopus Octopus vulgaris Cuvier, 1798 is extremely important in fisheries and is a useful protein source in most Mediterranean countries. Here we investigated pathogens associated with skin lesions in 9 naturally deceased specimens that included both cultured and wild common octopus. Within 30 min after death, each octopus was stored at 4°C and microbiologically examined within 24 h. Bacterial colonies, cultured from swabs taken from the lesions, were examined using taxonomical and biochemical analyses. Vibrio alginolyticus and V. parahaemolyticus were only isolated from cultured animals. A conventional PCR targeting the 16S ribosomal RNA (rRNA) gene and sequencing were performed on 2 bacterial isolates that remained unidentified after taxonomical and biochemical analysis. The sequence results indicated that the bacteria had a 99% identity with Lactococcus garvieae and Photobacterium swingsii. L. garvieae was confirmed using a specific PCR based on the 16S-23S rRNA internal transcribed spacer region, while P. swingsii was confirmed by phylogenetic analyses. Although all animals examined were found to be infected by the protozoan species Aggregata octopiana localised in the intestines, it was also present in skin lesions of 2 of the animals. Betanodavirus was detected in both cultured and wild individuals by cell culture, PCR and electron microscopy. These findings are the first report of L. garvieae and betanodavirus from skin lesions of common octopus and the first identification of P. swingsii both in octopus skin lesions and in marine invertebrates in Italy. Farto et al. (2003) isolated V. len tus from gills, branchial-heart and skin lesions of wild common octopus in Spain, confirming its pathogenic potential.Aggregata spp. (Protozoa: Apicomplexa) coccidian parasites are considered the dominant epizootiological agents in wild and cultured octopus stocks in Europe (Gestal et al. 2002a(Gestal et al. , 2007. In severe forms, Aggregata spp. infection spreads from the digestive tract to the mantle and the gills, affecting the connective tissue and epithelium of these organs and causing the appearance of white cysts associated with host cell hypertrophy with nuclear displacement, inflammation, phagocytosis, ulceration, and destruction of the organ architecture (Gestal et al. 2002b, CastellanosMartínez & Gestal 2013. A. octopiana is the only species of this genus reported in common octopus (Estévez et al. 1996, Castellanos-Martínez et al. 2013.Viruses infect most marine organisms, from bacteria to invertebrates and vertebrates (Pierce et al. 2010). Cephalopods, including common octopus, have been found to contain virus-like particles, probably of the genus Iridovirus, associated with tumours in the muscle tissue of the arms and, in advanced stages, on the funnel and the ventral surface of the mantle (Farley 1978, Hanlon & Forsythe 1990.The aim of the present study was to investigate the pathogens associated with skin lesions in cultured and wild common octopus. MATERIALS AND METHODS AnimalsBetw...

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“…VNN attacks can be transmitted vertically from an infected mother VNN [9] or horizontally through infected fish [16][17] as well as from the rest of the fish (trash fish) or molluscs used as feedin the aquaculture [18]. VNN infected fish show the symptoms such as abnormal swimming behavior (circling or whirling), floats above the stomach, decreased appetite and body color gel [15].The virus concentrate in the retina, brain, and spinal cord of infected fish.…”

Section: Introductionmentioning

confidence: 99%

Genetic Characterization of Viral Nervous Necrosis Infects Tilapia (Oreochromis sp.) in Indonesia

Yanuhar¹,

Christi²,

Arfiati³

2018

Proceedings of the 1st International Conference in One Health (ICOH 2017)

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Tilapia (Oreochromis sp.) is the most important cultured fish in the 21 century and Indonesia is the country's second largest tilapia producer after China. It make Indonesia should be more concerned with any possible attack diseases that can infect both from the class of parasites, fungi, bacteria, and viruses especially Virus Nervous Necrosis (VNN). The existence of VNN in tilapia is one of the problems that must be handled. In the present research, a natural outbreak of VNN in tilapia (Oreochromis sp.) farmed in mid-Java in Indonesia was investigated using molecular genetic characterization. Research conducted using Reverse transcriptase PCR (RT-PCR) methods, sequencing analysis, and phylogenetic analysis. the construction of phylogenetic trees is based on the neighbor-joining method using software MEGA6. The samples used are a tilapia juvenile size 5-7 cm, with or no clinical symptoms, and taken at random in the cultivation of tilapia in Central Java. It showed that the tilapia juvenile infected VNN show changes and damage to the organs and tissues. It can be seen from the examination of clinical symptoms, anatomic pathology and histopathology. DNA of the tilapia infected by VNN was 294 bp on both the brain and the eyes organ. Phylogenetic analysis results showed that VNN from infected tilapia was the same as snapper and grouper and has the closest kinship with genotype Redspotted grouper nervous necrosis virus (RGNNV), which is about 99%. It can be stated that Betanodavirus in freshwater fish potential is derived from Betanodavirus in seawater fish.

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Trash fish can be a source of betanodaviruses for cultured marine fish

Cited by 49 publications

References 27 publications

Betanodavirus of Marine and Freshwater Fish: Distribution, Genomic Organization, Diagnosis and Control Measures

Betanodavirus of Marine and Freshwater Fish: Distribution, Genomic Organization, Diagnosis and Control Measures

Skin lesion-associated pathogens from Octopus vulgaris: first detection of Photobacterium swingsii, Lactococcus garvieae and betanodavirus

Genetic Characterization of Viral Nervous Necrosis Infects Tilapia (Oreochromis sp.) in Indonesia

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