Trapping red blood cells in living animals using optical tweezers

Zhong, Min; Wei, Xunbin; Zhou, Jinhua; Wang, Ziqiang; Li, Yinmei

doi:10.1038/ncomms2786

Cited by 334 publications

(200 citation statements)

References 30 publications

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“…In our opinion, this could open the route for potential impacts either for future blood diagnostics and in vivo endoscopy. Recent improvement in biophotonics as the manipulation of RBCs from the outside of the vein 16 and, at the same time, new papers on the miniaturization of OT on a single fibre 17,43,44 could convincingly lead to envisage an integration of the presented technique for endoscopy. For example, it could be investigated a fibre-based system able to manipulate and trap RBCs inside the vein.…”

Section: Discussionmentioning

confidence: 99%

“…In particular, challenging results have been obtained by enlarging the potential capabilities of optical tweezers (OTs) as a no-contact tool for cell manipulation [13][14][15] . Recently, for the first time, optical trapping has been aimed in vivo at manipulating RBCs within subdermal capillaries on living mice 16 . At the same time, many efforts have been spent to go towards the miniaturization of a photonic device that could open new ways for endoscopic analysis [17][18][19] .…”

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confidence: 99%

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Red blood cell as an adaptive optofluidic microlens

et al. 2015

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The perspective of using live cells as lenses could open new revolutionary and intriguing scenarios in the future of biophotonics and biomedical sciences for endoscopic vision, local laser treatments via optical fibres and diagnostics. Here we show that a suspended red blood cell (RBC) behaves as an adaptive liquid-lens at microscale, thus demonstrating its imaging capability and tunable focal length. In fact, thanks to the intrinsic elastic properties, the RBC can swell up from disk volume of 90 fl up to a sphere reaching 150 fl, varying focal length from negative to positive values. These live optofluidic lenses can be fully controlled by triggering the liquid buffer's chemistry. Real-time accurate measurement of tunable focus capability of RBCs is reported through dynamic wavefront characterization, showing agreement with numerical modelling. Moreover, in analogy to adaptive optics testing, blood diagnosis is demonstrated by screening abnormal cells through focal-spot analysis applied to an RBC ensemble as a microlens array.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Red blood cell as an adaptive optofluidic microlens

et al. 2015

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“…[129][130][131][132][133] Optical tweezers manipulate cells with very high precision and possibility to spatially arrange cells. Microscale objects are forced by optical forces towards the focus point of a laser beam, where the trapped objects can be repositioned in all dimensions by moving the beam and changing focus.…”

Section: Optical Trapsmentioning

confidence: 99%

Microfluidics cell sample preparation for analysis: Advances in efficient cell enrichment and precise single cell capture

et al. 2017

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Single cell analysis has received increasing attention recently in both academia and clinics, and there is an urgent need for effective upstream cell sample preparation. Two extremely challenging tasks in cell sample preparation-highefficiency cell enrichment and precise single cell capture-have now entered into an era full of exciting technological advances, which are mostly enabled by microfluidics. In this review, we summarize the category of technologies that provide new solutions and creative insights into the two tasks of cell manipulation, with a focus on the latest development in the recent five years by highlighting the representative works. By doing so, we aim both to outline the framework and to showcase example applications of each task. In most cases for cell enrichment, we take circulating tumor cells (CTCs) as the target cells because of their research and clinical importance in cancer. For single cell capture, we review related technologies for many kinds of target cells because the technologies are supposed to be more universal to all cells rather than CTCs. Most of the mentioned technologies can be used for both cell enrichment and precise single cell capture. Each technology has its own advantages and specific challenges, which provide opportunities for researchers in their own area. Overall, these technologies have shown great promise and now evolve into real clinical applications. Published by AIP Publishing. [http://dx

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“…pressure; AFM indentation [7], which is similar to cell injection technique; optical tweezers [8], also known as laser traps, in which a single cell is stretched by optical forces or a laser beam; microarrays for investigation of local cellular environment [9], which can also be used to measure cellular response to environmental stimuli; and Magnetic Twisting Cytometry (MTC) [10,11] and oscillatory magnetic twisting cytometry [1], which are two recent techniques similar to optical tweezers.…”

Section: Introductionmentioning

confidence: 99%

Application of Hyperelastic Models in Mechanical Properties prediction of Mouse Oocyte and Embryo Cells at Large Deformations

et al. 2017

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Abstract. Biological cell studies have many applications in biology, cell manipulation, and diagnosis of diseases such as cancer and malaria. In this study, Inverse Finite Element Method (IFEM) combined with Levenberg-Marquardt optimization algorithm has been used to extract and characterize material properties of mouse oocyte and embryo cells at large deformations. Then, the simulation results have been validated using data from experimental works. In this study, it is assumed that cell material is hyperelastic, isotropic, homogenous, and axisymmetric. For inverse analysis, FEM model of cell injection experiment implemented in Abaqus software has been coupled with Levenberg-Marquardt optimization algorithm written in Matlab; through this coupling, the optimum hyperelastic coe cients, which give the best match between experimental and simulated forces, are extracted. Results show that among di erent hyperelastic material models, Ogden material is suitable for characterization of mouse oocyte cell and Mooney-Rivlin or polynomial is suitable for characterization of mouse embryo cell. Moreover, the evaluated Poisson ratio of the cell is obtained to be equal to 0.5, which indicates that the structural materials of mouse oocyte and embryo are compressible.

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Trapping red blood cells in living animals using optical tweezers

Cited by 334 publications

References 30 publications

Red blood cell as an adaptive optofluidic microlens

Red blood cell as an adaptive optofluidic microlens

Microfluidics cell sample preparation for analysis: Advances in efficient cell enrichment and precise single cell capture

Application of Hyperelastic Models in Mechanical Properties prediction of Mouse Oocyte and Embryo Cells at Large Deformations

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