Trapping of 4-hydroxynonenal by glutathione efficiently prevents formation of DNA adducts in human cells

Falletti, Olivier; Cadet, Jean; Favier, Alain; Douki, Thierry

doi:10.1016/j.freeradbiomed.2007.01.024

Cited by 44 publications

(38 citation statements)

References 55 publications

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“…The mercapturic acid pathway has been reported as one of the major routes to metabolize endogenous and xenobiotic electrophiles, such as 4-hydroxy-2-nonenal, an ␣,␤-unsaturated aldehyde generated from lipid peroxidation, and sulforphane (SFN), a naturally occurring isothiocyanate present in cruciferous vegetables (Kassahun et al, 1997;Hayes and McLellan, 1999;Falletti et al, 2007;Pernice et al, 2009;Ahn et al, 2010;Rudd et al, 2011). The ␣,␤-unsaturated keto group of [6]-shogaol makes it a good substrate for thiol conjugation.…”

Section: Discussionmentioning

confidence: 99%

Metabolism of [6]-Shogaol in Mice and in Cancer Cells

Chen

Lv²,

Soroka³

et al. 2012

Drug Metab Dispos

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ABSTRACT:Ginger has received extensive attention because of its antioxidant, antiinflammatory, and antitumor activities. However, the metabolic fate of its major components is still unclear. In the present study, the metabolism of [6]-shogaol, one of the major active components in ginger, was examined for the first time in mice and in cancer cells. Thirteen metabolites were detected and identified, seven of which were purified from fecal samples collected from

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Section: Discussionmentioning

confidence: 99%

Metabolism of [6]-Shogaol in Mice and in Cancer Cells

Chen

Lv²,

Soroka³

et al. 2012

Drug Metab Dispos

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“…Glutathione S-transferases are involved in the detoxification of electrophilic compounds, including products of lipid peroxidation, by conjugation with glutathione (Falletti et al, 2007). Gstp1 indentified together with Prdx5 in the increased spot 9 was significantly upregulated on the mRNA level in the presence of microglia-conditioned medium.…”

Section: Discussionmentioning

confidence: 99%

Activated microglia modulate astroglial enzymes involved in oxidative and inflammatory stress and increase the resistance of astrocytes to oxidative stress in Vitro

Röhl

Armbrust

Kolbe³

et al. 2008

Glia

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Neuropathological processes in the central nervous system are commonly accompanied by an activation of microglia and astrocytes. The involvement of both cell populations in the onset and progress of neurological disorders has been widely documented, implicating both beneficial and detrimental influences on the neural tissue. Nevertheless, little is known about the interplay of these glial cell populations, especially under diseased conditions. To examine the effects of activated microglia on astrocytes purified rat astroglial cell cultures were treated with medium conditioned by purified quiescent (MCM[-]) or lipopolysaccharide (LPS)-activated rat microglia (MCM[+]) and subjected to a comparative proteome analysis based on two-dimensional gel electrophoresis. No significant down regulation of proteins was observed. The majority of the 19 proteins identified by means of nano HPLC/ESI-MS/MS in the 12 most prominent protein spots significantly overexpressed (> or =2-fold) in MCM[+] treated astrocytes are involved in inflammatory processes and oxidative stress response: superoxide dismutases (Sod), peroxiredoxins, glutathione S-transferases (Gst), nucleoside diphosphate kinase B, argininosuccinate synthase (Ass), and cellular retinol-binding protein I (Rbp1). Sod2, Rbp1, Gstp1, and Ass were also significantly increased on the mRNA level determined by quantitative RT-PCR. The upregulation of antioxidative enzymes in astrocytes was accompanied by a higher resistance to oxidative stress induced by H2O2. These results show that activated microglia change the expression of antioxidative proteins in astrocytes and protect them against oxidative stress, which might be an effective way to increase the neuroprotective potential of astrocytes under pathological conditions associated with oxidative stress and inflammation.

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“…37 At 30 minutes after cells had been exposed to H 2 O 2 , intracellular levels of GSH decreased significantly and at 6 hours and 24 hours recovered to baseline levels. The time-dependent recovery of GSH levels was abolished by adding anti-RLIP76 C-ter antibodies to the medium.…”

Section: Effects Of Anti-rlip76 C-ter Antibodies On Intracellular 4-hmentioning

confidence: 90%

Autoantibodies to the C-terminal subunit of RLIP76 induce oxidative stress and endothelial cell apoptosis in immune-mediated vascular diseases and atherosclerosis

Margutti¹,

Matarrese

Conti³

et al. 2008

Blood

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Although detection of autoantibodies in the peripheral blood from patients with immune-mediated endothelial dysfunctions has so far failed to provide tools of diagnostic or pathogenetic value, putative bioindicators include anti-endothelial cell antibodies, a heterogeneous family of antibodies that react with autoantigens expressed by endothelial cells. In this study, to identify endothelial autoantigens involved in the autoimmune processes causing endothelial damage, we screened a human microvascular endothelial cell cDNA library with sera from patients with Behç et's disease. We identi-

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Trapping of 4-hydroxynonenal by glutathione efficiently prevents formation of DNA adducts in human cells

Cited by 44 publications

References 55 publications

Metabolism of [6]-Shogaol in Mice and in Cancer Cells

Metabolism of [6]-Shogaol in Mice and in Cancer Cells

Activated microglia modulate astroglial enzymes involved in oxidative and inflammatory stress and increase the resistance of astrocytes to oxidative stress in Vitro

Autoantibodies to the C-terminal subunit of RLIP76 induce oxidative stress and endothelial cell apoptosis in immune-mediated vascular diseases and atherosclerosis

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