Trapping and imaging of micron‐sized embryos using dielectrophoresis

Khoshmanesh, Khashayar; Kiss, Nimrod B.; Nahavandi, Saeid; Evans, Clive W.; Cooper, Jonathan M.; Williams, David E.; Wlodkowic, Donald

doi:10.1002/elps.201100160

Cited by 24 publications

(17 citation statements)

References 22 publications

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“…For example, the curved microelectrodes can be developed in extruded [15] or bottom-top patterned [16] configurations to produce stronger DEP force in a larger area of the micro-environment in tandem with hydrodynamic forces. Alternatively, the curved microelectrodes can be scaled down to sort and trap small bacteria or even cell organelles, or scaled up to trap and image the multi-cellular and embryonic organisms [38].…”

Section: Discussionmentioning

confidence: 99%

Dielectrophoresis of micro/nano particles using curved microelectrodes

et al. 2011

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Dielectrophoresis, the induced motion of polarisable particles in non-homogenous electric field, has been proven as a versatile mechanism to transport, immobilise, sort and characterise micro/nano scale particle in microfluidic platforms. The performance of dielectrophoretic (DEP) systems depend on two parameters: the configuration of microelectrodes designed to produce the DEP force and the operating strategies devised to employ this force in such processes. This work summarises the unique features of curved microelectrodes for the DEP manipulation of target particles in microfluidic systems. The curved microelectrodes demonstrate exceptional capabilities including (i) creating strong electric fields over a large portion of their structure, (ii) minimising electro-thermal vortices and undesired disturbances at their tips, (iii) covering the entire width of the microchannel influencing all passing particles, and (iv) providing a large trapping area at their entrance region, as evidenced by extensive numerical and experimental analyses. These microelectrodes have been successfully applied for a variety of engineering and biomedical applications including (i) sorting and trapping model polystyrene particles based on their dimensions, (ii) patterning carbon nanotubes to trap low-conductive particles, (iii) sorting live and dead cells based on their dielectric properties, (iv) real-time analysis of drug-induced cell death, and (v) interfacing tumour cells with environmental scanning electron microscopy to study their morphological properties. The DEP systems based on curved microelectrodes have a great potential to be integrated with the future lab-on-achip systems.

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Section: Discussionmentioning

confidence: 99%

Dielectrophoresis of micro/nano particles using curved microelectrodes

et al. 2011

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“…The curved microelectrodes create a strong spatially varying electric field within the microchannel, which is crucial to create dielectrophoresis. The curved microelectrodes have distinct advantages over other configurations, as reported in (Khoshmanesh et al 2011c;Khoshmanesh et al 2010b). They generate a strong electric field and maintain it over a large portion of their structure.…”

Section: Theoretical Backgroundmentioning

confidence: 93%

“…Dielectrophoresis has been widely applied in microfluidic systems to manipulate, transport, separate and characterise bio-particles, including multicellular organisms (Khoshmanesh et al 2011b), mammalian cells (Khoshmanesh et al 2011a;Kang et al 2008), bacteria (Hu et al 2005), viruses (Park et al 2007) and cell organelles (Moschallski et al 2010), as comprehensively reviewed in (Khoshmanesh et al 2011c;Pethig 2010).…”

Section: Introductionmentioning

confidence: 99%

On-chip separation of Lactobacillus bacteria from yeasts using dielectrophoresis

Khoshmanesh

Baratchi

Tovar-Lopez

et al. 2011

Microfluid Nanofluid

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Dielectrophoresis, the induced motion of dielectric particles in non-uniform electric fields, enables the separation of suspended bio-particles based on their dimensions or dielectric properties. This work presents a microfluidic system, which utilises a combination of dielectrophoretic (DEP) and hydrodynamic drag forces to separate Lactobacillus bacteria from a background of yeasts. The performance of the system is demonstrated at two operating frequencies of 10 MHz and 100 kHz. At 10 MHz, we are able to trap the yeasts and bacteria at different locations of the microelectrodes as they experience different magnitudes of DEP force. Alternatively, at 100 kHz we are able to trap the bacteria along the microelectrodes, while repelling the yeasts from the microelectrodes and washing them away by the drag force. These separation mechanisms might be applicable to automated lab-on-a-chip systems for the rapid and label-free separation of target bio-particles.

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“…Manipulation of biological particles (DNA, virus, bacteria, cell, multicellular organisms, etc.) is highly important in various biomedical and biotechnological applications . Recently, tremendous amount of research has been done to design micro‐/nanofluidic platforms to manipulate biological particles (whose size ranging from 10 nm to 100 μm) using electrokinetic (EK) forces .…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoretic separation of bioparticles in microdevices: A review

2014

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In recent years, dielectrophoretic force has been used to manipulate colloids, inert particles, and biological microparticles, such as red blood cells, white blood cells, platelets, cancer cells, bacteria, yeast, microorganisms, proteins, DNA, etc. This specific electrokinetic technique has been used for trapping, sorting, focusing, filtration, patterning, assembly, and separating biological entities/particles suspended in a buffer medium. Dielectrophoretic forces acting on particles depend on various parameters, for example, charge of the particle, geometry of the device, dielectric constant of the medium and particle, and physiology of the particle. Therefore, to design an effective micro-/nanofluidic separation platform, it is necessary to understand the role of the aforementioned parameters on particle motion. In this paper, we review studies particularly related to dielectrophoretic separation in microfluidic devices. Both experimental and theoretical works by several researchers are highlighted in this article covering AC and DC DEP. In addition, AC/DC DEP, which uses a combination of low frequency AC and DC voltage to manipulate bioparticles, has been discussed briefly. Contactless DEP, a variation of DC DEP in which electrodes do not come in contact with particles, has also been reviewed. Moreover, dielectrophoretic force-based field flow fractionations are featured to demonstrate the bioparticle separation in microfluidic device. In numerical front, a comprehensive review is provided starting from the most simplified effective moment Stokes-drag (EMSD) method to the most advanced interface resolved method. Unlike EMSD method, recently developed advanced numerical methods consider the size and shape of the particle in the electric and flow field calculations, and these methods provide much more accurate results than the EMSD method for microparticles.

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Trapping and imaging of micron‐sized embryos using dielectrophoresis

Cited by 24 publications

References 22 publications

Dielectrophoresis of micro/nano particles using curved microelectrodes

Dielectrophoresis of micro/nano particles using curved microelectrodes

On-chip separation of Lactobacillus bacteria from yeasts using dielectrophoresis

Dielectrophoretic separation of bioparticles in microdevices: A review

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