Transverse Relaxation Time of Leaf Water Protons and Membrane Injury in Wheat (Triticum aestivum L.) in Response to High Temperature

Maheswari, M.; Joshi, Deeksha; Saha, Ramkrishna; Nagarajan, S.; Gambhir, P. N.

doi:10.1006/anbo.1999.0974

Cited by 42 publications

(30 citation statements)

References 26 publications

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“…In our recent study, a close correlation of water content with water component indicated by T 1 and T 2 for long and short fractions was observed in grains of rice grown in the outdoor (Ishibashi et al, 2005). Moreover, T 1 and T 2 of water protons have been applied to the studies of higher plant tissues exposed to thermal stresses (Maheswari et al, 1999;Iwaya-Inoue et al, 2004a, b).…”

mentioning

confidence: 68%

Influence of Low/High Temperature on Water Status in Developing and Maturing Rice Grains

Funaba

Ishibashi

Molla

et al. 2006

Plant Production Science

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mentioning

confidence: 68%

Influence of Low/High Temperature on Water Status in Developing and Maturing Rice Grains

Funaba

Ishibashi

Molla

et al. 2006

Plant Production Science

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“…The T2 component relaxing at 3 ms in oilseed rape leaves was not observed because of the relatively long pulse space and the very small number of points sampled for the CPMG curve. Finally, in the study of Maheswari et al (1999), the CPMG decay curve was acquired over 250 ms, which was not long enough for reliable fitting of the curve, since the baseline was not reached (last point , 1.4 3 T 2-max ). On the other hand, Capitani et al (2009) investigated the leaves of several species using a portable unilateral NMR apparatus (18.153 MHz) characterized by an inhomogenous magnetic field that did not allow measurement of the FID signal and produced shortening of the T2 values measured.…”

Section: Interpretation Of the Nmr Signal Of Senescing Leavesmentioning

confidence: 99%

Structural Changes in Senescing Oilseed Rape Leaves at Tissue and Subcellular Levels Monitored by Nuclear Magnetic Resonance Relaxometry through Water Status

et al. 2013

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Nitrogen use efficiency is relatively low in oilseed rape (Brassica napus) due to weak nitrogen remobilization during leaf senescence. Monitoring the kinetics of water distribution associated with the reorganization of cell structures, therefore, would be valuable to improve the characterization of nutrient recycling in leaf tissues and the associated senescence processes. In this study, nuclear magnetic resonance (NMR) relaxometry was used to describe water distribution and status at the cellular level in different leaf ranks of well-watered plants. It was shown to be able to detect slight variations in the evolution of senescence. The NMR results were linked to physiological characterization of the leaves and to light and electron micrographs. A relationship between cell hydration and leaf senescence was revealed and associated with changes in the NMR signal. The relative intensities and the transverse relaxation times of the NMR signal components associated with vacuole water were positively correlated with senescence, describing water uptake and vacuole and cell enlargement. Moreover, the relative intensity of the NMR signal that we assigned to the chloroplast water decreased during the senescence process, in agreement with the decrease in relative chloroplast volume estimated from micrographs. The results are discussed on the basis of water flux occurring at the cellular level during senescence. One of the main applications of this study would be for plant phenotyping, especially for plants under environmental stress such as nitrogen starvation.The main physiological outcome of leaf senescence is the recycling of organic resources and the provision of nutrients to sink organs such as storage and growing tissues (Buchanan-Wollaston, 1997;Hikosaka, 2005;Krupinska and Humbeck, 2008). In crop plants, senescence progresses from the lower older leaves to the younger top leaves. Macromolecular degradation and the mechanism of reallocation of breakdown products are mediated by the up-regulation of senescence-related genes (Lee et al., 2001) in close relationship with both developmental and environmental conditions (Gombert et al., 2006). This leads to remobilization of carbon and nitrogen (N) compounds mostly from plastidial compartments (Martínez et al., 2008;Guiboileau et al., 2012), involving proteolytic activity in plasts, vacuole, and cytosol (Adam and Clarke, 2002;Otegui et al., 2005), chlorophyll breakdown (Hoertensteiner, 2006), galactolipid recycling (Kaup et al., 2002) in the plastoglobules (Brehelin et al., 2007), and loading of Suc and amino acids into the phloem through appropriate transporters (Wingler et al., 2004;Masclaux-Daubresse et al., 2008). In terms of leaf senescence at the cell level, where chloroplasts are degraded sequentially, relative organelle volume does not seem to be greatly modified, the vacuole remains intact, and in darkness-induced senescence the number of chloroplasts per cell decreases only slightly (Keech et al., 2007). However, major changes in metabolic fluxes and cell water...

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“…Applied on a wide leaf panel collected from plant grown in both controlled and field conditions, the low field NMR relaxometry was shown to provide precise and robust markers of development of oilseed rape leaves [30]. NMR relaxation is thus a powerful tool to monitor changes in water relations inside the leaf caused by development or different stress as Nitrogen deficiency [35] or thermal stress [36]. Only a few studies have focused on the impact of water stress on the NMR relaxation signal of the leaf [37,38].…”

Section: Introductionmentioning

confidence: 99%

Leaf Development Monitoring and Early Detection of Water Deficiency by Low Field Nuclear Magnetic Resonance Relaxation in Nicotiana tabacum Plants

et al. 2018

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Drought is the main abiotic stress worldwide affecting harvest quality and quantity of numerous crops. To enable better water management, low field NMR (nuclear magnetic resonance) relaxometry was assessed as a developmental marker and a new method for early detection of water deficiency. The effect of a foliar biostimulant against water stress was also investigated. Two leaves of different ranks (four and eight) were studied. The leaves of different ranks were characterized by different NMR T 2 spectra which validated the ability of NMR to describe the developmental stage of tobacco. Results also showed that T 2 NMR relaxation spectra allow the detection of mild water stress (80% of the field capacity) through the precise characterization of the leaf water status while other water stress markers (relative water content, photosynthetic related parameters . . . )were not yet impacted. The agricultural impact of the mild water stress was determined through the nitrogen rate in shoots and amino acids assay six weeks after the beginning of the stress and results shows that foliar application of biostimulant limits the negative consequences of drought. Our results demonstrate the sensitivity of NMR to detect slight changes triggered in the leaf by water stress at the tissue level.

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Transverse Relaxation Time of Leaf Water Protons and Membrane Injury in Wheat (Triticum aestivum L.) in Response to High Temperature

Cited by 42 publications

References 26 publications

Influence of Low/High Temperature on Water Status in Developing and Maturing Rice Grains

Influence of Low/High Temperature on Water Status in Developing and Maturing Rice Grains

Structural Changes in Senescing Oilseed Rape Leaves at Tissue and Subcellular Levels Monitored by Nuclear Magnetic Resonance Relaxometry through Water Status

Leaf Development Monitoring and Early Detection of Water Deficiency by Low Field Nuclear Magnetic Resonance Relaxation in Nicotiana tabacum Plants

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