Transvaginal, Sonographically Controlled Ovarian Follicle Puncture for Egg Retrieval

Dellenbach, P; Nisand, I.; Moreau, Laurence; Feger, B.; C, Plumere; Gerlinger, P; Buisson, B.; Rumpler, Y.

doi:10.1016/s0140-6736(84)91958-5

Cited by 89 publications

(22 citation statements)

References 4 publications

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“…One of the most common procedures in clinical settings is the retrieval of metaphase II (MII)-stage oocytes from the ovarian follicles by ultrasound-guided transvaginal aspiration, ovum pick-up (OPU) 1 . These oocytes are then fertilized in vitro (IVF), with the resulting embryo(s) implanted in a recipient uterus.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Franciosi

Tessaro

Dalbiès-Tran

et al. 2017

JoVE

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The field of assisted reproduction has been developed to treat infertility in women, companion animals, and endangered species. In the horse, assisted reproduction also allows for the production of embryos from high performers without interrupting their sports career and contributes to an increase in the number of foals from mares of high genetic value. The present manuscript describes the procedures used for collecting immature and mature oocytes from horse ovaries using ovum pick-up (OPU). These oocytes were then used to investigate the incidence of aneuploidy by adapting a protocol previously developed in mice. Specifically, the chromosomes and the centromeres of metaphase II (MII) oocytes were fluorescently labeled and counted on sequential focal plans after confocal laser microscope scanning. This analysis revealed a higher incidence in the aneuploidy rate when immature oocytes were collected from the follicles and matured in vitro compared to in vivo. Immunostaining for tubulin and the acetylated form of histone four at specific lysine residues also revealed differences in the morphology of the meiotic spindle and in the global pattern of histone acetylation. Finally, the expression of mRNAs coding for histone deacetylases (HDACs) and acetyl-transferases (HATs) was investigated by reverse transcription and quantitative-PCR (q-PCR). No differences in the relative expression of transcripts were observed between in vitro and in vivo matured oocytes. In agreement with a general silencing of the transcriptional activity during oocyte maturation, the analysis of the total transcript amount can only reveal mRNA stability or degradation. Therefore, these findings indicate that other translational and post-translational regulations might be affected.

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Section: Introductionmentioning

confidence: 99%

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Franciosi

Tessaro

Dalbiès-Tran

et al. 2017

JoVE

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“…The findings of this report highlight the risk of complications after transvaginal invasive procedures with the use of needles and demonstrate the importance of hygiene procedures, the need to employ a needle for each procedure and the importance of a trained person to perform a safe procedure. KEYWORDS: Bovines, ovary, reproduction, ultrasonography, transvaginal technique, TruCut needle INTRODUÇÃO O emprego de técnicas transvaginais para coleta de material ovariano foi relatado pela primeira vez em estudos voltados à colheita de oócitos para a realização de fertilização in-vitro (FIV) em mulheres inférteis (GLEICHER et al, 1983;DELLENBACH et al, 1984). Nas vacas, também com a mesma finalidade, o acesso ovariano por via transvaginal com auxílio de ultrassonografia começou a ser empregado na década seguinte (PIETERSE et al, 1991) e hoje é uma prática amplamente difundida.…”

Section: Peritonitis After Ultrasonography Guided Biopsy Of Corpus Luunclassified

Peritonite Após Biópsia De Corpo Lúteo Guiada Por Ultrassonografia Em Vacas Da Raça Holandesa: Relato De Caso

Dalanezi¹,

García²,

Ferrazza³

et al. 2015

Enciclopédia Biosfera

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RESUMOEm bovinos, os procedimentos invasivos ovarianos transvaginais, empregados com finalidades diagnósticas, biotecnológicas ou experimentais, mesmo sendo práticas consolidadas, podem apresentar complicações posteriores. Este relato tem como objetivo apresentar complicações observadas em três fêmeas bovinas da raça Holandesa submetidas à biópsia de corpo lúteo (CL) pela técnica transvaginal guiada por ultrassonografia, empregando-se agulha Tru-cut (18G x 48 cm). No segundo dia após o procedimento, os animais começaram a apresentar sinais característicos de peritonite, como leucocitose, neutrofilia, hiperfibrinogenemia, acúmulo de fluido intra-abdominal e aderências do útero e ovários às vísceras circunvizinhas. Como o prognóstico desta afecção é ruim ou reservado, as vacas foram eutanasiadas e necropsiadas. Os principais achados post mortem foram: hidroperitôneo, peritonite fibrinosa difusa, ooforite fibrinosa associada a múltiplos cistos ovarianos, acúmulo de líquido seroso/purulento abundante no útero e múltiplos cistos aderidos à serosa intestinal, além de aderências intestinais ao ovário. Como o grau de comprometimento peritoneal era decrescente e pelo fato dos animais acometidos terem sido submetidos à biópsia de CL sequencialmente no mesmo dia, com a mesma agulha, suspeitou-se que no primeiro animal pode ter ocorrido o acidente primário que resultou na contaminação fecal da agulha, sendo disseminada para os demais animais. Os achados do presente relato demonstram o risco de ocorrência de complicações após procedimentos invasivos de fundo vaginal com o emprego de agulhas e sinalizam a importância dos cuidados de higiene, da troca da agulha e a necessidade de experiência do operador para a realização segura da técnica.

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“…18 Later Schwimer and Lebovic in the mid-1980s introduced the technical improvements that provided TVS with its powerful diagnostic and therapeutic properties. 19,20 They used 3.5MHz transabdominal transducers via the transvaginal route. This technique did not gain wide acceptance because it did not result in substantial improvement in image resolution.…”

Section: Historical Perspectivementioning

confidence: 99%

Application of ultrasonography in female infertility: a comprehensive review

Okeke

Agwuna

Ezenyeaku

et al. 2015

Int J Reprod Contracept Obstet Gynecol

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Transvaginal, Sonographically Controlled Ovarian Follicle Puncture for Egg Retrieval

Cited by 89 publications

References 4 publications

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

Peritonite Após Biópsia De Corpo Lúteo Guiada Por Ultrassonografia Em Vacas Da Raça Holandesa: Relato De Caso

Application of ultrasonography in female infertility: a comprehensive review

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