Transthyretin synthesis in rabbit ciliary pigment epithelium

Kawaji, Takahiro; Ando, Yukio; Nakamura, Masaaki; Yamamoto, Keiichi; Ando, Eiko; Takano, Akiomi; Inomata, Yasuya; Hirata, Akira; Tanihara, Hidenobu

doi:10.1016/j.exer.2005.02.003

Cited by 63 publications

(40 citation statements)

References 20 publications

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“…14 Moreover, in endotoxin-induced uveitis (EIU) in a rabbit model, intraocular TTR production by CPE was downregulated until 48 hours after the induction of EIU. 15 In our study, however, ocular TTR was overexpressed in patients with JIA-uveitis and silent chronic AU with active disease. This discrepancy might be explained by the fact that inflammation in JIA-associated uveitis does not display an acute, but a chronic process with an insidious onset and a silent course.…”

Section: Discussionmentioning

confidence: 51%

“…It has been suggested from studies demonstrating TTR production in rabbit CPE cells that TTR expression might contribute to the formation of amyloid fibrils in the pupillary margin and angle chamber. 15 In addition, TTR was reported to be increased in the AH of eyes with myopia and POAG but under expressed in eyes with primary congenital glaucoma. 5,7,[19][20][21] Grus et al proposed TTR as a biomarker for POAG, based on the upregulation of a 14,132 Da TTR species in the AH of POAG eyes.…”

Section: Discussionmentioning

confidence: 99%

“…15 TTR is present in almost all ocular tissues in noninflammatory eyes, including AH. [16][17][18] TTR in the vitreous has been demonstrated not to originate from the blood.…”

Section: Discussionmentioning

confidence: 99%

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Intraocular Biomarker Identification in Uveitis Associated With Juvenile Idiopathic Arthritis

Ayuso

Boer

Byers

et al. 2013

Invest. Ophthalmol. Vis. Sci.

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PURPOSE.To investigate the presence of biomarkers in aqueous humor (AH) from patients with uveitis associated with juvenile idiopathic arthritis (JIA).METHODS. AH (n ¼ 73) and serum (n ¼ 105) samples from 116 children were analyzed using surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-ToF MS). The samples were divided into the following groups: JIA, silent chronic anterior uveitis (AU), other uveitis entities, and noninflammatory controls. Statistical biomarker identification was performed using the SELDI-ToF Biomarker Analysis Cluster Wizard followed by multivariate statistical analysis. Biochemical identification of biomarkers was performed by polyacrylamide gel protein separation, followed by liquid chromatography tandem mass spectrometry. ELISA was performed in a number of AH samples representing all four study groups.RESULTS. In the JIA group, one AH protein peak at mass/charge (m/z) 13,762 had qualitative and quantitative differences in expression compared with the other uveitis entities and the controls, but not to the group of silent chronic AU. Its quantitative expression in AH of patients with JIA and other silent chronic AU was positively associated with uveitis activity. The protein at m/z 13,762 in AH was identified as transthyretin (TTR). The TTR concentration in AH differed significantly between the study groups (P ¼ 0.006) with considerably higher TTR concentrations in JIA and silent chronic AU samples positive for m/z 13,762 than those of the other uveitis and control groups.CONCLUSIONS. TTR is a potential intraocular biomarker of JIA-associated uveitis. Its role in the pathogenesis of silent chronic AU with and without arthritis needs further investigation.

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Section: Discussionmentioning

confidence: 51%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Intraocular Biomarker Identification in Uveitis Associated With Juvenile Idiopathic Arthritis

Ayuso

Boer

Byers

et al. 2013

Invest. Ophthalmol. Vis. Sci.

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“…There are some theoretical explanations about elevated TTR levels in glaucomatous AH. In animal experiments, TTR synthesis in ciliary pigment epithelium was shown (125) . This location of TTR synthesis could explain the local infl uence of this protein in the anterior chamber of the eye without having a systemic effect.…”

Section: Transthyretinmentioning

confidence: 99%

Biomarkers in primary open angle glaucoma

Kokotas

Kroupis

Chiras

et al. 2012

Clinical Chemistry and Laboratory Medicine (CCLM)

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Glaucoma, a leading cause of blindness worldwide, is currently defi ned as a disturbance of the structural or functional integrity of the optic nerve that causes characteristic atrophic changes in the optic nerve, which may lead to specifi c visual fi eld defects over time. This disturbance usually can be arrested or diminished by adequate lowering of intraocular pressure (IOP). Glaucoma can be divided roughly into two main categories, ' open angle ' and ' closed angle ' glaucoma. Open angle, chronic glaucoma tends to progress at a slower rate and patients may not notice loss of vision until the disease has progressed signifi cantly. Primary open angle glaucoma (POAG) is described distinctly as a multifactorial optic neuropathy that is chronic and progressive with a characteristic acquired loss of optic nerve fi bers. Such loss develops in the presence of open anterior chamber angles, characteristic visual fi eld abnormalities, and IOP that is too high for the healthy eye. It manifests by cupping and atrophy of the optic disc, in the absence of other known causes of glaucomatous disease. Several biological markers have been implicated with the disease. The purpose of this study was to summarize the current knowledge regarding the non-genetic molecular markers which have been predicted to have an association with POAG but have not yet been validated.

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“…1 In situ hybridization analysis of rabbit eyes demonstrated the site of TTR production in the retinal pigment epithelium and the ciliary pigment epithelium. 2 A mutation of the TTR gene located on the 18th chromosome (18q12.1) is responsible for the development of the disease, and is inherited in an autosomal dominant pattern. 3 The mutation results in a structural alteration causing misfolding or aggregation of the TTR and its precipitation in tissues.…”

mentioning

confidence: 99%