Transrectal fine needle aspiration biopsy of the prostate combining cytomorphologic, DNA ploidy status and cell cycle distribution studies

Paz-Bouza, J.I.; Órfão, Alberto; Abad, Mónica Viñarás; Ciudad, Juana; García, M. Cano; López, Antonio; Bullón, A

doi:10.1016/s0344-0338(11)80747-8

Cited by 21 publications

(11 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For the immunophenotypic flow cytometry analyses, single tumor cell suspensions were obtained from 51 freshly-frozen meningioma samples (46 WHO grade I, 3 WHO grade II and 2 WHO grade III tumors), through conventional mechanical disaggregation procedures [14], [15] in phosphate buffered saline (PBS) containing 10% fetal bovine serum (FBS; Invitrogen, Carlsbad, CA, USA), 1% bovine serum albumin (BSA) (Sigma, St. Louis, MO, USA) and 2 mM EDTA (Merck, Darmstadt, Germany). Thawed meningioma cells were stained for 30 min at 4°C in the darkness with the following monoclonal antibodies (mAb): CD2- fluorescein isothiocyanate (FITC), CD13- phycoerythrin (PE), CD14-PE, CD33-PE, CD58-PE, CD69-PE, HER2/neu-PE and HLA-DR-FITC, purchased from Becton/Dickinson (BD) Biosciences (BDB, San Jose, CA, USA); CD22-FITC, CD37-FITC, CD53-PE, CD55-FITC, CD81-PE and CD99-PE from BD Pharmigen (San Diego, CA, USA); CD9-FITC, CD16-FITC, CD63-FITC and HLA-I-FITC purchased from Beckman/Coulter (Fullerton, CA, USA); CD44-PE and CD59-FITC obtained from Immunostep SL (Salamanca, Spain); Bcl2-FITC and CD45-Pacific Blue (PacB) purchased from DAKO (Glostrup, Denmark); CD38-FITC from Cytognos SL (Salamanca, Spain), and; CD68-FITC obtained from An der Grub (ADG, Vienna, Austria).…”

Section: Methodsmentioning

confidence: 99%

Association between Inflammatory Infiltrates and Isolated Monosomy 22/del(22q) in Meningiomas

et al. 2013

View full text Add to dashboard Cite

Meningiomas contain highly variable levels of infiltrating tissue macrophages (TiMa) and other immune cells. In this study we investigated the potential association between the number and immunophenotype of inflammatory and other immune cells infiltrating the tumor as evaluated by multiparameter flow cytometry, and the clinico-biological, cytogenetic and gene expression profile (GEP) of 75 meningioma patients. Overall, our results showed a close association between the amount and cellular composition of the inflammatory and other immune cell infiltrates and the cytogenetic profile of the tumors. Notably, tumors with isolated monosomy 22/del(22q) showed greater numbers of TiMa, NK cells and (recently)-activated CD69+ lymphocytes versus meningiomas with diploid and complex karyotypes. In addition, in the former cytogenetic subgroup of meningiomas, tumor-infiltrating TiMa also showed a more activated and functionally mature phenotype, as reflected by a greater fraction of CD69+, CD63+, CD16+ and CD33+ cells. GEP at the mRNA level showed a unique GEP among meningiomas with an isolated monosomy 22/del(22q) versus all other cases, which consisted of increased expression of genes involved in inflammatory/immune response, associated with an M1 TiMa phenotype. Altogether, these results suggest that loss of expression of specific genes coded in chromosome 22 (e.g. MIF) is closely associated with an increased homing and potentially also anti-tumoral effect of TiMa, which could contribute to explain the better outcome of this specific good-prognosis cytogenetic subgroup of meningiomas.

show abstract

Section: Methodsmentioning

confidence: 99%

Association between Inflammatory Infiltrates and Isolated Monosomy 22/del(22q) in Meningiomas

et al. 2013

View full text Add to dashboard Cite

show abstract

“…Samples corresponding to a macroscopically tumoral region were cut, placed in saline solution, and sent to the FISH laboratory for further analysis of numerical chromosome abnormalities. Immediately upon receipt, single-cell suspensions were obtained from the samples using a well-established automated mechanical disaggregation procedure (37,38). Cells were then resuspended in a 3/1 methanol/acetic (vol/vol) and stored at -20°C until analysis.…”

Section: Fish Studiesmentioning

confidence: 99%

Incidence of numerical chromosome aberrations in meningioma tumors as revealed by fluorescence in situ hybridization using 10 chromosome‐specific probes

et al. 2002

View full text Add to dashboard Cite

Objective: Although information on the cytogenetic characteristics of meningioma tumors has accumulated progressively over the past few decades, information on the genetic heterogeneity of meningiomas is still scanty. The aim of the present study was to analyze by interphase fluorescence in situ hybridization (FISH) the incidence of numerical abnormalities for chromosomes 1, 9, 10, 11, 14, 15, 17, 22, X, and Y in a group of 70 consecutive meningioma tumors. Another goal was to establish the potential associations among the altered chromosomes, as a way to assess both intertumoral and intratumoral heterogeneity. Methods: For the purpose of the study, 70 patients diagnosed with meningioma were analyzed. Interphase FISH for the detection of numerical abnormalities for chromosomes 1, 9, 10, 11, 14, 15, 17, 22, X, and Y was applied to fresh tumor samples from each of the patients studied. Results: The overall incidence of numerical abnormalities was 76%. Chromosome Y in males and chromosome 22 in the whole series were the most common abnormalities (46% and 61%, respectively). Despite the finding that monosomy of chromosome 22/22q -deletions are the most frequent individual abnormality (53%), we have observed that chromosome gains are significantly more common than chromosome losses (60% versus 40%). Chromosome gains corresponded to abnormalities of chromosomes 1 (27%), 9 (25%), 10 (23%), 11 (22%), 14 (33%), 15 (22%), 17 (23%), and X in females (35%) and males (23%) whereas chromosome losses apart from chromosome 22 frequently involved chromosomes 14 (19%), X in males (23%), and Y in males (32%). Although an association was found among most gained chromosomes on one side and chromosome losses on the other side, different association patterns were observed. Furthermore, in the latter group, monosomy 22/22q -was associated with monosomy X in females and monosomy 14/14q -was associated with nulisomy Y in males. In addition, chromosome losses usually involved a large proportion of the tumor cells whereas chromosome gains were restricted to small tumor cell clones, including tetraploid cells. Conclusions: Our results show that meningiomas are genetically heterogeneous tumors that display different patterns of numerical chromosome changes, as assessed by interphase FISH. Cytometry (Clin. Cytometry) 50:153-159, 2002.

show abstract

“…Employing well-established mechanical disaggregation procedures (García García et al 1992;Paz-Bouza et al 1994;Lizana et al 2000), single-cell suspensions were obtained. These were immediately centrifuged at 540g for 5 min and the cell pellet frozen (-20°C, 5 days maximum) until used.…”

Section: Dna Cell Content Flow Cytometry Studiesmentioning

confidence: 99%

Quantitative evaluation of the distribution of proliferating cells in the adult retina in three cyprinid species

Cid

Velasco

Ciudad

et al. 2002

Cell and Tissue Research

View full text Add to dashboard Cite

In the present study, a descriptive and quantitative analysis of all the proliferating cell populations present in the normal adult retina of three cyprinid species (goldfish, zebrafish, and tench) is reported. Evaluation of cell proliferation was performed in proliferating cell nuclear antigen (PCNA)-labeled tissue sections as well as in single-cell suspensions analyzed by flow cytometry. Our results show that the neural progenitors of the inner nuclear layer (INL) of cyprinids continue dividing in adulthood in uninjured retinas. These cells are probably related to the generation of rods in normal retinal growth, as well as in the production of any retinal cell type in regenerating processes. The distributions of both these cells and their presumptive progeny, the rod precursors, differ from one species to another, being homogeneous in zebrafish, displaced to the periphery in goldfish and to the temporal pole in tench. With regard to the cell apposition at the retinal periphery, it seems to be symmetrical in goldfish and zebrafish, based on a homogeneous extension of the peripheral growth zone (PGZ), but asymmetrical in tench, where it presents a significantly lower extension in the ventral retina. The flow cytometry analyses indicate that, overall, the proportion of proliferating cells is significantly greater in zebrafish retina despite the fact that body growth rate is lower in zebrafish than the other two species.

show abstract

Transrectal fine needle aspiration biopsy of the prostate combining cytomorphologic, DNA ploidy status and cell cycle distribution studies

Cited by 21 publications

References 30 publications

Association between Inflammatory Infiltrates and Isolated Monosomy 22/del(22q) in Meningiomas

Association between Inflammatory Infiltrates and Isolated Monosomy 22/del(22q) in Meningiomas

Incidence of numerical chromosome aberrations in meningioma tumors as revealed by fluorescence in situ hybridization using 10 chromosome‐specific probes

Quantitative evaluation of the distribution of proliferating cells in the adult retina in three cyprinid species

Contact Info

Product

Resources

About