Transposon Tn 10

Schlör, Stefan; Kemmer, G.; Reidl, Joachim

doi:10.1385/1-59259-321-6:211

Cited by 3 publications

(3 citation statements)

References 15 publications

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“…Transposon mutagenesis in H. influenzae was accomplished according to a method described by Schlör et al . 44 . For this purpose, 480 independent transformations with pAKcat carrying the transposon Tn 10d-cat were performed in Rd AK01.…”

Section: Methodsmentioning

confidence: 99%

“…Interesting mutants with stronger or weaker OMV, OMP-P1, -P2, -P4, -P5 and/or -P6 signals compared with the wild-type were validated once. Then, chromosomal Tn 10d-cat insertions were located by DNA sequencing of junction fragments generated by PCR using the transposon mutagenesis oligonucleotide primers, which are listed in Supplementary Table 4 , and a previously described method 44 46 .…”

Section: Methodsmentioning

confidence: 99%

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A novel mechanism for the biogenesis of outer membrane vesicles in Gram-negative bacteria

et al. 2016

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Bacterial outer membrane vesicles (OMVs) have important biological roles in pathogenesis and intercellular interactions, but a general mechanism of OMV formation is lacking. Here we show that the VacJ/Yrb ABC (ATP-binding cassette) transport system, a proposed phospholipid transporter, is involved in OMV formation. Deletion or repression of VacJ/Yrb increases OMV production in two distantly related Gram-negative bacteria, Haemophilus influenzae and Vibrio cholerae. Lipidome analyses demonstrate that OMVs from VacJ/Yrb-defective mutants in H. influenzae are enriched in phospholipids and certain fatty acids. Furthermore, we demonstrate that OMV production and regulation of the VacJ/Yrb ABC transport system respond to iron starvation. Our results suggest a new general mechanism of OMV biogenesis based on phospholipid accumulation in the outer leaflet of the outer membrane. This mechanism is highly conserved among Gram-negative bacteria, provides a means for regulation, can account for OMV formation under all growth conditions, and might have important pathophysiological roles in vivo.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

A novel mechanism for the biogenesis of outer membrane vesicles in Gram-negative bacteria

et al. 2016

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“…For transposon mutagenesis in H. influenzae, 5 independent Tn10d-cat transposon insertions pools were generated in microtiter plates, according to a method which was described by Schlör et al (2003), yielding in about 8000 retransformed chloramphenicol-resistant colonies. To this end, 50 ml of mid-log phase H. influenzae AK01 cells were transformed with 50-100 ng of purified pAKcat plasmid, encoding Tn10d-cat (Kraiß et al, 1998) and coincubated for 30 min and then plated onto sBHI agar plates containing chloramphenicol.…”

Section: Tn10d-cat Mutagenesis and Paraquat Selectionmentioning

confidence: 99%

Transposon insertion in a serine-specific minor tRNA coding sequence affects intraperitoneal survival of Haemophilus influenzae in the infant rat model

Gerlach

Anthony

Deadman

et al. 2010

International Journal of Medical Microbiology

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Mobile Genetic Elements as Natural Tools for Genome Evolution

Miller

Capy

Mobile Genetic Elements

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Transposable elements (TEs) are ubiquitous components of all living organisms, and in the course of their coexistence with their respective host genomes, these parasitic DNAs have played important roles in the evolution of complex genetic networks. The interaction between mobile DNAs and their host genomes are quite diverse, ranging from modifications of gene structure and regulation to alterations in general genome architecture. Thus over evolutionary time these elements can be regarded as natural molecular tools in shaping the organization, structure, and function of eukaryotic genes and genomes. Based on their intrinsic properties and features, mobile DNAs are widely applied at present as a technical "toolbox," essential for studying a diverse spectrum of biological questions. In this chapter we aim to review both the evolutionary impact of TEs on genome evolution and their valuable and diverse methodological applications as the molecular tools presented in this book.

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Transposon Tn 10

Cited by 3 publications

References 15 publications

A novel mechanism for the biogenesis of outer membrane vesicles in Gram-negative bacteria

A novel mechanism for the biogenesis of outer membrane vesicles in Gram-negative bacteria

Transposon insertion in a serine-specific minor tRNA coding sequence affects intraperitoneal survival of Haemophilus influenzae in the infant rat model

Mobile Genetic Elements as Natural Tools for Genome Evolution

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