2023
DOI: 10.1101/2023.03.29.534805
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Transposon-sequencing across multiple Mycobacterium abscessus isolates reveals significant functional genomic diversity among strains

Abstract: Mycobacterium abscessus (Mab) clinical isolates have large accessory genomes that are comprised of plasmids, prophages, and genomic islands acquired by horizontal gene transfer. Due to their large accessory genomes, Mab clinical isolates are highly genetically diverse. The functional consequence of this diversity remains largely unknown because to date, functional genomic studies in Mab have largely been performed on reference strains. Given the growing public health threat of Mab infections, understanding the… Show more

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Cited by 2 publications
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“…As the field progresses, there is certain to be an increased embrace of experimental diversity—both in the utilization of different bacterial and host strains, as well as in the types of genetic interactions studied. Instances of this trend are increasingly evident: for example, a pooled CRISPRi screen was used to compare gene vulnerability in M. tuberculosis HN878, a clinical strain, with the standard laboratory strain, M. tuberculosis H37Rv, and revealed differing vulnerabilities in drug targets (Bosch et al, 2021 ), echoing results in M. abscessus in which a Tn screen identified differential essentiality in 563 genes across 22 clinical isolates (Akusobi et al, 2023 ) and E. coli in which differences in gene essentiality were investigated across 18 strains under three conditions (Rousset et al, 2021 ).…”
Section: Future Directions: Embracing Complexitymentioning
confidence: 99%
“…As the field progresses, there is certain to be an increased embrace of experimental diversity—both in the utilization of different bacterial and host strains, as well as in the types of genetic interactions studied. Instances of this trend are increasingly evident: for example, a pooled CRISPRi screen was used to compare gene vulnerability in M. tuberculosis HN878, a clinical strain, with the standard laboratory strain, M. tuberculosis H37Rv, and revealed differing vulnerabilities in drug targets (Bosch et al, 2021 ), echoing results in M. abscessus in which a Tn screen identified differential essentiality in 563 genes across 22 clinical isolates (Akusobi et al, 2023 ) and E. coli in which differences in gene essentiality were investigated across 18 strains under three conditions (Rousset et al, 2021 ).…”
Section: Future Directions: Embracing Complexitymentioning
confidence: 99%