“…Mutagenic SB transposons were developed to modify expression of endogenous genes in specific ways depending on their relative position and orientation, as previously described [8]. This feature, combined with our experience analyzing insertion site data from 15 SB-induced models of cancer [2, 3, 20, 29, 30, 33, 34, 37, 40, 41, 45–47, 52, 55], led us to develop an algorithm to predict the functional impact that recurrent transposon insertion has on a given gene. This approach evaluates clustered transposon insertions within each candidate locus to determine if there is a bias for insertion in the same orientation as the gene, indicative of an over-expression mechanism, or if the transposons are randomly orientated, suggesting a gene disruption mechanism (see Additional file 2: Supplemental Methods).…”