Transposon-mediated transgenesis in the frog: New tools for biomedical and developmental studies

Yergeau, Donald; Mead, Paul E.

doi:10.2741/3242

Cited by 3 publications

(2 citation statements)

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“…The recent identification of mutants of interest after gene‐breaking transposon mutagenesis and phenotypic screening (Petzold et al, 2009) suggests that large‐scale screening after insertional mutagenesis can be considered and that large collections will ultimately be available. Insertional mutagenesis was also described in Xenopus (Yergeau and Mead, 2009) but, again, no large collection has been developed yet.…”

Section: Methods Derived From Forward Genetics: Random Modifications mentioning

confidence: 99%

Reverse genetics in eukaryotes

Hardy

Legagneux

Audic

et al. 2010

Biology of the Cell

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Reverse genetics consists in the modification of the activity of a target gene to analyse the phenotypic consequences. Four main approaches are used towards this goal and will be explained in this review. Two of them are centred on genome alterations. Mutations produced by random chemical or insertional mutagenesis can be screened to recover only mutants in a specific gene of interest. Alternatively, these alterations may be specifically targeted on a gene of interest by HR (homologous recombination). The other two approaches are centred on mRNA. RNA interference is a powerful method to reduce the level of gene products, while MO (morpholino) antisense oligonucleotides alter mRNA metabolism or translation. Some model species, such as Drosophila, are amenable to most of these approaches, whereas other model species are restricted to one of them. For example, in mice and yeasts, gene targeting by HR is prevalent, whereas in Xenopus and zebrafish MO oligonucleotides are mainly used. Genome‐wide collections of mutants or inactivated models obtained in several species by these approaches have been made and will help decipher gene functions in the post‐genomic era.

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Section: Methods Derived From Forward Genetics: Random Modifications mentioning

confidence: 99%

Reverse genetics in eukaryotes

Hardy

Legagneux

Audic

et al. 2010

Biology of the Cell

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“…The fourth group of reporter lines are transposable element enhancer trap lines, all generated by the Mead lab (Supplementary Table S1D; Yergeau and Mead, 2009; Yergeau et al, 2010). Although the integration sites for some of these lines have been identified, this is not the case for all of them.…”

Section: Transgenic Resourcesmentioning

confidence: 99%

Xenopus Resources: Transgenic, Inbred and Mutant Animals, Training Opportunities, and Web-Based Support

Horb

Wlizla

Abu-Daya³

et al. 2019

Front. Physiol.

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Two species of the clawed frog family, Xenopus laevis and X. tropicalis , are widely used as tools to investigate both normal and disease-state biochemistry, genetics, cell biology, and developmental biology. To support both frog specialist and non-specialist scientists needing access to these models for their research, a number of centralized resources exist around the world. These include centers that hold live and frozen stocks of transgenic, inbred and mutant animals and centers that hold molecular resources. This infrastructure is supported by a model organism database. Here, we describe much of this infrastructure and encourage the community to make the best use of it and to guide the resource centers in developing new lines and libraries.

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