Transposition of Tn1831 to sym plasmids of Rhizobium leguminosarum and Rhizobium trifolii

Pees, E.

doi:10.1016/0378-1097(86)90231-4

Cited by 5 publications

(1 citation statement)

References 19 publications

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“…Transferring the rhizobial Sym plasmid into Agrobacterium tumefaciens and Philobacterium myrsinacearum also results in a capacity to induce root nodule formation (Hooykaas et al, 1981(Hooykaas et al, , 1982Rodriquez-Quinones et al, 1989; van Veen et al, 1988). The development of genetic tools, such as the availability of broad-host range cloning and cosmid vectors (Ditta et al, 1980;Friedman et al , 1982), construction of cosmid libraries, complementation and mapping of nodulation-deficient mutants, introduction of random and directed transposon mutagenesis techniques coupled to marker exchange Buchanan-Wollaston, 1979;Meade et al, 1982) or used in combination with phage transduction methods (Pees et al, 1986;Wijffelman et al, 1985), led to the identification of regions involved in nodulation and, subsequently the first nodulation genes (Djordjevic et al, 1985;Downie et al, 1983Downie et al, ,1985Fisher et al, 1985;Kondorosi et al, 1984;Rossen et al, 1984;Schofield et al, 1984;Torok et al, 1984). DNA sequence analysis, as well as complementation studies, revealed that some nodulation genes are conserved in all rhizobia, whereas others are restricted to a few or a single species or strain.…”

Section: La Nodulation Genesmentioning

confidence: 99%

Genetic Organization and Transcriptional Regulation of Rhizobial Nodulation Genes

Schlaman¹,

Phillips²,

Kondorosi³

1998

The Rhizobiaceae

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Section: La Nodulation Genesmentioning

confidence: 99%

Genetic Organization and Transcriptional Regulation of Rhizobial Nodulation Genes

Schlaman¹,

Phillips²,

Kondorosi³

1998

The Rhizobiaceae

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Cloning and Characterization of Four Genes of Rhizobium leguminosarum bv. trifolii Involved in Exopolysaccharide Production and Nodulation

Workum

Cremers²,

Wijfjes³

et al. 1997

MPMI

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Four different genes of Rhizobium leguminosarum bv. trifolii strain RBL5599 involved in exopolysaccharide (EPS) production were identified by complementation of Tn5-induced EPS-deficient mutants (Exo mutants) with a cosmid bank. On one cosmid pssA was located, which was found to be almost identical to the pss4 gene from R. leguminosarum bv. viciae VF39 and highly homologous to a family of glycosyl transferases. Two pssA mutants, exo2 and exo4, were characterized and found to produce 19 and 1% of the wild-type amount of EPS, respectively. The three other genes were found to be closely linked on a different complementing cosmid. pssC revealed similarity to exoM and exoW of R. meliloti, both encoding glucosyl transferases involved in the synthesis of succinoglycan. A mutation in this gene (mutant exo50) did reduce EPS synthesis to 27% of the wild-type amount. We found an operon closely linked to pssC, consisting of two overlapping genes, pssD and pssE, that is essential for EPS production. Homology of pssD and pssE was found with cps14F and cps14G of Streptococcus pneumoniae, respectively: two genes responsible for the second step in capsule polysaccharide synthesis. Furthermore, pssD and pssE were homologous to the 5' and 3' parts, respectively, of spsK of Sphingomonas S88, which encodes a putative glycosyl transferase. Structural analysis of EPS produced by Exo mutants exo2, exo4, and exo50 showed it to be identical to that of the parental strain RBL5599, with the exception of acetyl groups esterified to one of the glucose residues being absent.

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Role of Exopolysaccharides of Rhizobium leguminosarum bv. viciae as Host Plant-Specific Molecules Required for Infection Thread Formation During Nodulation of Vicia sativa

Workum¹,

Slageren²,

Brussel³

et al. 1998

MPMI

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Mutants of Rhizobium leguminosarum bv. viciae bacteria that are affected in the biosynthesis of exopolysaccharides (EPS) are unable to effectively nodulate their host plants. By studying defined mutants, we show that R. legumi-nosarum bv. viciae strains require EPS for formation of infection threads in Vicia sativa (vetch) as well as for efficient induction of tight root hair curling. Results of coinoculation experiments with the EPS-deficient pssD111 mutant of R. leguminosarum bv. viciae in combination with heterologous EPS-producing strains indicated that vetch has certain structural requirements for rhizobial EPS to function in symbiosis. We hypothesize that EPS accelerates root hair curling and infection to such an extent that rhizobial root penetration precedes a plant defense response.

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Transposition of Tn1831 to sym plasmids of Rhizobium leguminosarum and Rhizobium trifolii

Cited by 5 publications

References 19 publications

Genetic Organization and Transcriptional Regulation of Rhizobial Nodulation Genes

Genetic Organization and Transcriptional Regulation of Rhizobial Nodulation Genes

Cloning and Characterization of Four Genes of Rhizobium leguminosarum bv. trifolii Involved in Exopolysaccharide Production and Nodulation

Role of Exopolysaccharides of Rhizobium leguminosarum bv. viciae as Host Plant-Specific Molecules Required for Infection Thread Formation During Nodulation of Vicia sativa

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