Transposition of IS
            <i>911</i>

Rousseau, Philippe; Normand, Christophe; Loot, Céline; Turlan, Catherine; Alazard, Robert; Duval‐Valentin, Guy; Chandler, Michaël

doi:10.1128/9781555817954.ch16

Cited by 22 publications

(37 citation statements)

References 76 publications

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“…Bacterial members of these families are thought to transpose by a mechanism involving a replication step to eject a circular IS transposition copy from the donor site, which then serves as a transposition intermediate (78). In the case of the IS3 family member IS911, this process has been shown to depend on the DnaG primase (22).…”

Section: Is Distribution In Archaea Compared To Bacteria and Eukaryamentioning

confidence: 99%

Insertion Sequence Diversity in Archaea

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Chandler

2007

Microbiol Mol Biol Rev

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SUMMARY Insertion sequences (ISs) can constitute an important component of prokaryotic (bacterial and archaeal) genomes. Over 1,500 individual ISs are included at present in the ISfinder database (www-is.biotoul.fr ), and these represent only a small portion of those in the available prokaryotic genome sequences and those that are being discovered in ongoing sequencing projects. In spite of this diversity, the transposition mechanisms of only a few of these ubiquitous mobile genetic elements are known, and these are all restricted to those present in bacteria. This review presents an overview of ISs within the archaeal kingdom. We first provide a general historical summary of the known properties and behaviors of archaeal ISs. We then consider how transposition might be regulated in some cases by small antisense RNAs and by termination codon readthrough. This is followed by an extensive analysis of the IS content in the sequenced archaeal genomes present in the public databases as of June 2006, which provides an overview of their distribution among the major archaeal classes and species. We show that the diversity of archaeal ISs is very great and comparable to that of bacteria. We compare archaeal ISs to known bacterial ISs and find that most are clearly members of families first described for bacteria. Several cases of lateral gene transfer between bacteria and archaea are clearly documented, notably for methanogenic archaea. However, several archaeal ISs do not have bacterial equivalents but can be grouped into Archaea-specific groups or families. In addition to ISs, we identify and list nonautonomous IS-derived elements, such as miniature inverted-repeat transposable elements. Finally, we present a possible scenario for the evolutionary history of ISs in the Archaea.

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Section: Is Distribution In Archaea Compared To Bacteria and Eukaryamentioning

confidence: 99%

Insertion Sequence Diversity in Archaea

Filée

Siguier

Chandler

2007

Microbiol Mol Biol Rev

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131

134

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“…There are two open reading frames, orfA (from 82 to 333 bp) and orfB (from 387 to 1229 bp) identified on the ISLC3 sequence, which are in phase 0 and 21, respectively. A conserved DD(35)E triad has also been identified in the middle of the OrfB sequence, suggesting the existence of a catalytic domain on the sequence (Rousseau et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…ISs are grouped into more than 20 families based on the conservation of motifs of the putative transposase and terminal nucleotide sequences. The IS3 family is the largest group identified so far, with more than 90 members isolated from over 26 genera and 51 species (Mahillon & Chandler, 1998;Rousseau et al, 2002). To date, only a few members of the IS3 family have been characterized in lactobacilli, e.g.…”

Section: Introductionmentioning

confidence: 99%

Formation of an inverted repeat junction in the transposition of insertion sequence ISLC3 isolated from Lactobacillus casei

Chen

Tsai

et al. 2008

Microbiology

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An insertion sequence, ISLC3, of 1351 bp has been isolated from Lactobacillus casei. Formation of IS circles containing a 3 bp spacer (complete junction) or deletion of 25 bp at the left inverted repeat (IRL) between the abutted IS ends of the ISLC3 junction region (deleted junction) was also discovered in the lactobacilli and Escherichia coli system studied. We found that the promoter formed by the complete junction P jun was more active than that formed by the 25 bp deleted junction P djun or the indigenous promoter P IRL . The corresponding transcription start sites for both promoter P jun and P IRL as well as P djun were subsequently determined using a primer extension assay. The activity of transposase OrfAB of ISLC3 was also assayed using an in vitro system. It was found that this transposase preferred to cleave a single DNA strand at the IRR over the IRL end in the transposition process, suggesting that attack of one end by the other was oriented from IRR to IRL.

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“…IS1236 belongs to the large IS3 family, members of which transpose using a replicative excision and conservative integration mechanism, also known as the copy-andpaste method of transposition (15,18). As a result, the element is retained in its original locus as well as being inserted at a new location (8,15,30,37,38). Transposition can insert IS1236 elements at novel chromosomal positions that might enable gene duplication via homologous recombination, as depicted in Fig.…”

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Analysis of IS 1236 -Mediated Gene Amplification Events in Acinetobacter baylyi ADP1

et al. 2012

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Recombination between insertion sequence copies can cause genetic deletion, inversion, or duplication. However, it is difficult to assess the fraction of all genomic rearrangements that involve insertion sequences. In previous gene duplication and amplification studies of Acinetobacter baylyi ADP1, an insertion sequence was evident in approximately 2% of the characterized duplication sites. Gene amplification occurs frequently in all organisms and has a significant impact on evolution, adaptation, drug resistance, cancer, and various disorders. To understand the molecular details of this important process, a previously developed system was used to analyze gene amplification in selected mutants. The current study focused on amplification events in two chromosomal regions that are near one of six copies of the only transposable element in ADP1, IS 1236 (an IS 3 family member). Twenty-one independent mutants were analyzed, and in contrast to previous studies of a different chromosomal region, IS 1236 was involved in 86% of these events. IS 1236 -mediated amplification could occur through homologous recombination between insertion sequences on both sides of a duplicated region. However, this mechanism presupposes that transposition generates an appropriately positioned additional copy of IS 1236 . To evaluate this possibility, PCR and Southern hybridization were used to determine the chromosomal configurations of amplification mutants involving IS 1236 . Surprisingly, the genomic patterns were inconsistent with the hypothesis that intramolecular homologous recombination occurred between insertion sequences following an initial transposition event. These results raise a novel possibility that the gene amplification events near the IS 1236 elements arise from illegitimate recombination involving transposase-mediated DNA cleavage.

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Transposition of IS 911

Cited by 22 publications

References 76 publications

Insertion Sequence Diversity in Archaea

Insertion Sequence Diversity in Archaea

Formation of an inverted repeat junction in the transposition of insertion sequence ISLC3 isolated from Lactobacillus casei

Analysis of IS 1236 -Mediated Gene Amplification Events in Acinetobacter baylyi ADP1

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