Transport properties of puerarin and effect of Radix Angelicae Dahuricae extract on the transport of puerarin in Caco-2 cell model

Absorptive interactions of concurrent oral administration of (+)-catechin and puerarin in rats and the underlying mechanisms

“…These findings were consistent with the cell uptake results, which indicated that in the process of absorption, a significant interaction between (+)-C and Pue actually occurred in the small intestine, and an underlying mechanism might be determined by the transport characteristics of these two compounds when they are co-administered. Liang et al previously revealed that the transport properties of Pue were time-and concentration-dependent, which illustrated that passive transport might be the main pathway for Pue in Caco-2 cells [29] . In this study, we mainly focused on the transcellular transport properties of (+)-C, and this study is the first to demonstrate time and concentration effects on (+)-C transport in Caco-2 cells.…”

“…Additionally, when cyclosporin A or MK-571 were added into the AP or the BL sides, (+)-C and Pue PDR values were both decreased significantly. Liang et al revealed that P-gp or MRP drug-efflux pump inhibitors are involved in Pue transport, which would cause a decrease in its PDR [29] ; thus, it was elucidated that P-gp and MRP-2 were involved in the transcellular transport of (+)-C and Pue and that they are definitely substrates of these two drug-efflux pumps. Taken together, we provided evidence that (+)-C and Pue might be two competitive substrates of the MRP-2 and P-gp drug-efflux pumps, which led to their mutual influence when they were Table 3.…”

Effects of herbal drugs in Mahuang decoction and their main components on intestinal transport characteristics of Ephedra alkaloids evaluated by a Caco-2 cell monolayer model

“…The drugs mediated by the same transporter co-transported through the Caco-2 cells may exhibit competitive inhibition or promotion on their permeability. The drug-drug interactions might affect the activity and expression of P-gp transporter, which leads to interfering the intestinal absorption of drugs (Wang et al, 2009;Zhang et al, 2010Zhang et al, , 2012Liang et al, 2012). Moreover, CMA or other organic acids in Cassia twig reacts with alkaloids from Ephedra to produce hydrophobic double salts, which cannot be transferred by Caco-2 cells (Cui and Zhao, 2011;Xu et al, 2012).…”

Simultaneous determination of acetylpuerarin and puerarin in rat plasma by liquid chromatography–tandem mass spectrometry: Application to a pharmacokinetic study following intravenous and oral administration

“…Moreover, PUE has been shown to exert protective effects against cerebrovascular ischemia-reperfusion injury (CIRI) in vivo [3][4][5] and neuron damage induced by oxygen-glucose deprivation in vitro [6,7]. However, the clinical application of PUE is greatly limited because of its low oral bioavailability [8,9]. Acetylpuerarin (AP, Fig.…”