Transport Properties of Gramicidin A Ion Channel in a Free-Standing Lipid Bilayer Filled With Oil Inclusions

Tawfik, Harvey; Puza, Sevde; Seemann, Ralf; Fleury, Jean‐Baptiste

doi:10.3389/fcell.2020.531229

Cited by 7 publications

(7 citation statements)

References 43 publications

(81 reference statements)

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“…We developed a 3D–microchip that enables the formation of a free‐standing horizontal lipid bilayer at a desired position (see Figure ), similar to other platforms reported in the literature. [ 20,23,24 ] However, our chip allows a superior optical access due to the reduced optical distance between the glass bottom of the microfluidic device and the formed bilayer (between 30 and 100 μm, Figure 1). [ 20,23,24 ] Moreover, and in contrast to previous systems, this platform enables to measure quantitatively the phospholipid fluidity on LD via FRAP.…”

Section: Resultsmentioning

confidence: 99%

“…[20,23,24] However, our chip allows a superior optical access due to the reduced optical distance between the glass bottom of the microfluidic device and the formed bilayer (between 30 and 100 μm, Figure 1). [20,23,24] Moreover, and in contrast to previous systems, this platform enables to measure quantitatively the phospholipid fluidity on LD via FRAP. [25] To form a bilayer, the bottom channel of the microfluidic device is filled with buffer (Figure 1C).…”

Section: Lipid Bilayer Characterizationmentioning

confidence: 99%

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Lipid Droplets Embedded in a Model Cell Membrane Create a Phospholipid Diffusion Barrier

Puza

Caesar

Poojari

et al. 2022

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the endoplasmic reticulum (ER) membrane where neutral lipids are synthesized. While the molecular mechanisms of this process are unknown, it appears that LD biogenesis is following a threestage process: neutral lipid synthesis, lens formation (via intra-membrane lipid accumulation), and finally LD formation. [3] At relatively low concentrations, neutral lipids accumulate between the two leaflets of the ER bilayer to eventually form a lipid reservoir with a lens shape. [3,4] Numerical modeling suggests that such lenses could exist in the ER membrane with a size of tens of nanometers. [3,5,6] Upon the continuous production of neutral lipids, they accumulate into the reservoir which leads to a lipid lens growth. Above a certain size, this lipid lens becomes unstable and can lead to a spontaneous budding of the oily reservoir. [3,7] Ultimately, the droplet pinchoff from the membrane leads to its release into the cytosol. [8][9][10] LDs dynamically adapt to metabolic changes in the cell and balance uptake of free fatty acids by their esterification and storage as triglycerides, and consumption of triglycerides by the release of fatty acids under catabolic conditions. These essential metabolic functions of LDs are executed by proteins on the LD surface, many of which dynamically partition from the ER bilayer to the LD monolayer membrane. [11,12] Aberrant LD functions are implicated in numerous metabolic diseases such as obesity, diabetes,The ORCID identification number(s) for the author(s) of this article can be found under

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Section: Resultsmentioning

confidence: 99%

Section: Lipid Bilayer Characterizationmentioning

confidence: 99%

Lipid Droplets Embedded in a Model Cell Membrane Create a Phospholipid Diffusion Barrier

Puza

Caesar

Poojari

et al. 2022

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“…A PDMS 3D-chip was produced with a thin rectangular channel connected with a conical hole, made with a 3D printed method as detailed in. 35,36,52 The bottom micro-channel of the PDMS chip was filled with a drop of oil+lipid followed by the delay of 5 min for spreading. Then a water droplet was added to the conical hole.…”

Section: Methodsmentioning

confidence: 99%

Protein corona modulates interaction of spiky nanoparticles with lipid bilayers

Fleury

Werner²,

Guével³

et al. 2021

Journal of Colloid and Interface Science

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“…[53,54] Inspired by the structure of naturally occurring antibiotic gramicidin pores, synthetic peptide pores have been designed to act as antibiotic agents by rupturing the bacterial membranes. [53,55] Furthermore, peptide pores have been used for label-free sensing. [56] Advantages of peptide pores include their facile fabrication via solid phase synthesis, their ability to easily insert into membranes due to their small dimensions, and the high freedom in structural design.…”

Section: Protein and Peptide Poresmentioning

confidence: 99%

Functional Nanopores Enabled with DNA

et al. 2023

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Membrane‐spanning nanopores are used in label‐free single‐molecule sensing and next‐generation portable nucleic acid sequencing, and as powerful research tools in biology, biophysics, and synthetic biology. Naturally occurring protein and peptide pores, as well as synthetic inorganic nanopores, are used in these applications, with their limitations. The structural and functional repertoire of nanopores can be considerably expanded by functionalising existing pores with DNA strands and by creating an entirely new class of nanopores with DNA nanotechnology. This review outlines progress in this area of functional DNA nanopores and outlines developments to open up new applications.

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Transport Properties of Gramicidin A Ion Channel in a Free-Standing Lipid Bilayer Filled With Oil Inclusions

Cited by 7 publications

References 43 publications

Lipid Droplets Embedded in a Model Cell Membrane Create a Phospholipid Diffusion Barrier

Lipid Droplets Embedded in a Model Cell Membrane Create a Phospholipid Diffusion Barrier

Protein corona modulates interaction of spiky nanoparticles with lipid bilayers

Functional Nanopores Enabled with DNA

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