Transport of xylose and glucose in the xylose-fermenting yeast Pichia stipitis

Kilian, S. G.; Uden, N. van

doi:10.1007/bf00451629

Cited by 150 publications

(97 citation statements)

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“…However, it would be beneficial to express an active xylose transporter, which is capable of taking up xylose in the cell against a concentration gradient, for utilization of xylose below 1 g L −1 (6.7 mM). Such transporters are common among the natural xylose utilizing yeasts (Alcorn and Griffin, 1978;Does and Bisson, 1989;Kilian and van Uden, 1988;Kilian et al, 1993;Lucas and van Uden, 1986;Nobre et al, 1999;Weierstall et al, 1999). In addition, it would be desirable to express a transporter, which is specific for xylose and not inhibited by glucose.…”

Section: Discussionmentioning

confidence: 99%

Control of xylose consumption by xylose transport in recombinant Saccharomyces cerevisiae

Gárdonyi

Jeppsson

Lidén

et al. 2003

Biotech & Bioengineering

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Section: Discussionmentioning

confidence: 99%

Control of xylose consumption by xylose transport in recombinant Saccharomyces cerevisiae

Gárdonyi

Jeppsson

Lidén

et al. 2003

Biotech & Bioengineering

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“…At lower extracellular xylose concentrations however, transport could become a critical step because of the remarkably low substrate affinity of the X D H [68-97 mM, depending on the cofactor (Rizzi et al 1988)]. In contrast to S. cerevisiae, P. stipitis takes up xylose by active proton symport (Does and Bisson 1989;Kilian and van Uden 1988). This system allows intracellular accumulation of xylose and could be a prerequisite for efficient xylose metabolism.…”

Section: Discussionmentioning

confidence: 99%

Xylose fermentation by Saccharomyces cerevisiae

Kötter

Ciriacy

1993

Appl Microbiol Biotechnol

455

341

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We have performed a comparative study of xylose utilization in Saccharomyces cerevisiae transformants expressing two key enzymes in xylose metabolism, xylose reductase (XR) and xylitol dehydrogenase (XDH), and in a prototypic xylose-utilizing yeast, Pichia stipitis. In the absence of respiration (see text), baker's yeast cells convert half of the xylose to xylitol and ethanol, whereas P. stipitis cells display rather a homofermentative conversion of xylose to ethanol. Xylitol production by baker's yeast is interpreted as a result of the dual cofactor dependence of the XR and the generation of NADPH by the pentose phosphate pathway. Further limitations of xylose utilization in S. cerevisiae cells are very likely caused by an insufficient capacity of the nonoxidative pentose phosphate pathway, as indicated by accumulation of sedoheptulose-7-phosphate and the absence of fructose-l,6-bisphosphate and pyruvate accumulation. By contrast, uptake at high substrate concentrations probably does not limit xylose conversion in S. cerevisiae X Y L 1 / X Y L 2 transformants.

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“…Supplementation with glucose in a xylose fermentation can thus be used to enhance the yield of xylitol. The presence of small amounts of glucose in the medium has indeed improved xylitol yield from xylose in yeast fermentations [2][3][4]; however, high concentrations of glucose are known to inhibit xylose transport into the cell [5] and repress induction of relevant enzymes by xylose [6,7].…”

Section: Introductionmentioning

confidence: 99%

A model of xylitol production by the yeast Candida mogii

Tochampa

Sirisansaneeyakul

Vanichsriratana

et al. 2005

Bioprocess Biosyst Eng

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Production of xylitol from xylose in batch fermentations of Candida mogii ATCC 18364 is discussed in the presence of glucose as the cosubstrate. Various initial ratios of glucose and xylose concentrations are assessed for their impact on yield and rate of production of xylitol. Supplementation with glucose at the beginning of the fermentation increased the specific growth rate, biomass yield and volumetric productivity of xylitol compared with fermentation that used xylose as the sole carbon source. A mathematical model is developed for eventual use in predicting the product formation rate and yield. The model parameters were estimated from experimental observations, using a genetic algorithm. Batch fermentations, which were carried out with xylose alone and a mixture of xylose and glucose, were used to validate the model. The model fitted well with the experimental data of cell growth, substrate consumption and xylitol production.

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Transport of xylose and glucose in the xylose-fermenting yeast Pichia stipitis

Cited by 150 publications

References 10 publications

Control of xylose consumption by xylose transport in recombinant Saccharomyces cerevisiae

Control of xylose consumption by xylose transport in recombinant Saccharomyces cerevisiae

Xylose fermentation by Saccharomyces cerevisiae

A model of xylitol production by the yeast Candida mogii

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