Transport of sucrose by Saccharomyces cerevisiae

Mwesigye, Patrick K.; Barford, J. P.

doi:10.1016/0922-338x(94)90154-6

Cited by 12 publications

(11 citation statements)

References 17 publications

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“…This could mean that there is a sucrose transport system that is induced when the cells are adapted on sucrose for a long time (up to 14 days). This is in agreement with our earlier reports on sucrose metabolism in S. cerevisiae (3,14,17). It has been reported that under glucose repression, the secretion of internal invertase increases while the external invertase remains low and practically constant (12).…”

Section: And Discussionsupporting

confidence: 82%

“…When the cells are growing on sucrose alone, the specific growth and sugar uptake rates increased from 0.38 to 0.55 h-' and the specific sugar uptake increased from 14 to 19 mmol g-biomass-' h-' when the adaptation period was prolonged from 2 to 14 days (17). This is also true for the uptake of radiolabelled sucrose when the cells are adapted on sucrose for a long time (14). When the cells are growing on sucrose and glucose mixtures, the specific growthh rate is on average 0.45 h-' for unadapted cells.…”

Section: And Discussionsupporting

confidence: 50%

“…The strain of baker's yeast used in this work was Saccharomyces cerevisiae 248 UNSW 703100 (3,14,17). This yeast was maintained on malt agar slopes and stored at 4°C.…”

Section: Methodsmentioning

confidence: 99%

“…Most Saccharomyces cerevisiae strains have two forms of invertase: an external large invertase, which is a glycoprotein, and an internal small invertase (9,15). Some strains of S. cerevisiae have been reported to transport sucrose directly into the cell (14,17,20). …”

mentioning

confidence: 99%

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Mechanism of surcrose utilisation by Saccharomyces cerevisiae.

Mwesigye¹,

Barford²

1996

J. Gen. Appl. Microbiol.

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Saccharomyces cerevisiae was grown on different concentrations of sucrose and glucose mixtures after adapting on sucrose. The yeast cells were found to have two different mechanisms by which sucrose was utilised: hydrolysis outside the cell membrane and direct transport into the cells. The mechanism by which sucrose was utilised depended on the initial concentration of glucose in the mixture and the adaptation state of the cells. In both cases, glucose was utilised first and invertase secretion was repressed when the glucose concentration was higher than 2 g l-'. The major important finding in our results is that, for fully sucrose-adapted cells, even in the presence of a repressive glucose concentration, the yeast cells were able to utilise sucrose, and most importantly, without first being hydrolysed to its constituent monosaccharides. Previously, it has been thought that direct transport of sucrose contributes only a small part of the process of utilisation of sucrose, but as our results indicate it can be the major mechanism of sucrose utilisation in S. cerevisiae if the cells are adapted on sucrose for long periods, glucose concentrations in the medium are high and/or invertase activities are limiting.The initial steps in the utilisation of sugars by yeast is either the passage of intact sugar molecules across the cell membrane or the hydrolysis outside the cell followed by entry of all or some of the hydrolytic products into the cell. Sugar transport into microbial cells across the plasmalemma can take place by active transport, facilitated diffusion and group translocation (19).Sucrose utilisation involves its hydrolysis outside the yeast cell by extracellular enzyme invertase ($-D-fructosidase) into glucose and fructose which are then transported into the cells and metabolised. Most Saccharomyces cerevisiae strains have two forms of invertase: an external large invertase, which is a glycoprotein, and an internal small invertase (9,15). Some strains of S. cerevisiae have been reported to transport sucrose directly into the cell (14,17,20).

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Section: And Discussionsupporting

confidence: 82%

Section: And Discussionsupporting

confidence: 50%

“…The strain of baker's yeast used in this work was Saccharomyces cerevisiae 248 UNSW 703100 (3,14,17). This yeast was maintained on malt agar slopes and stored at 4°C.…”

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Mechanism of surcrose utilisation by Saccharomyces cerevisiae.

Mwesigye¹,

Barford²

1996

J. Gen. Appl. Microbiol.

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“…The uptake of [ 14 C]maltose or [ 14 C]sucrose was determined as described previously [Santos et al, 1982;Mwesigye and Barford, 1994;Stambuk et al, 1998]. Cells grown as described above for the fermentation assays were harvested during the exponential phase of growth, washed twice with cold distilled water, and suspended in 0.05 M succinate-Tris buffer (pH 5.0) at a cell density of 30 g/l.…”

Section: Transport Assaysmentioning

confidence: 99%

Sucrose Fermentation by <i>Saccharomyces cerevisiae</i> Lacking Hexose Transport

Batista

Miletti

Stambuk³

2004

Microb Physiol

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Sucrose is the major carbon source used by Saccharomyces cerevisiae during production of baker’s yeast, fuel ethanol and several distilled beverages. It is generally accepted that sucrose fermentation proceeds through extracellular hydrolysis of the sugar, mediated by the periplasmic invertase, producing glucose and fructose that are transported into the cells and metabolized. In the present work we analyzed the contribution to sucrose fermentation of a poorly characterized pathway of sucrose utilization by S. cerevisiae cells, the active transport of the sugar through the plasma membrane and its intracellular hydrolysis. A yeast strain that lacks the major hexose transporters (hxt1–hxt7 and gal2) is incapable of growing on or fermenting glucose or fructose. Our results show that this hxt-null strain is still able to ferment sucrose due to direct uptake of the sugar into the cells. Deletion of the AGT1 gene, which encodes a high-affinity sucrose-H⁺ symporter, rendered cells incapable of sucrose fermentation. Since sucrose is not an inducer of the permease, expression of the AGT1 must be constitutive in order to allow growth of the hxt-null strain on sucrose. The molecular characterization of active sucrose transport and fermentation by S. cerevisiae cells opens new opportunities to optimize yeasts for sugarcane-based industrial processes.

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Quantitative analysis of the regulation scheme of invertase expression in Saccharomyces cerevisiae

Herwig

Doerries

Marison

et al. 2001

Biotech & Bioengineering

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In Saccharomyces cerevisiae, the expression of invertase, which is the hydrolyzing enzyme of sucrose, is controlled by the presence of monosaccharides, such as glucose and fructose, and referred to as carbon catabolite repression. To date, efforts have been made to identify the mechanism by which cells sense extracellular monosaccharide concentrations and trigger the genes involved in the repression pathway. The aim of the present work was to quantitatively investigate the cellular regulation of invertase expression in the wild-type strain S. cerevisiae CEN.PK113-7D during batch growth containing mixed sugar substrates under different initial conditions. Because of the high frequency and accurate online analysis of multiple components, a tight control of invertase expression could be observed, and threshold concentrations of the monosaccharides for derepression could be determined to 0.5 gl(-1) for glucose and 2 gl(-1) for fructose. Also, the existence of a hitherto undescribed regulatory state, in which cells regulate invertase expression very precisely and operate over long periods at monosaccharide concentrations lower than the above thresholds, could be demonstrated. All experimental observations could be summarized in a formulation of the cellular regulation scheme of invertase expression. A simple kinetic model could show that the regulation scheme explains the observed behavior very well. Additionally, the model was able to explain consequences of the regulation on the global metabolism.

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Transport of sucrose by Saccharomyces cerevisiae

Cited by 12 publications

References 17 publications

Mechanism of surcrose utilisation by Saccharomyces cerevisiae.

Mechanism of surcrose utilisation by Saccharomyces cerevisiae.

Sucrose Fermentation by <i>Saccharomyces cerevisiae</i> Lacking Hexose Transport

Quantitative analysis of the regulation scheme of invertase expression in Saccharomyces cerevisiae

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