Transport of succinate by pseudomonas putida

Dubler, Robert E.; Toscano, William A.; Hartline, Richard A.

doi:10.1016/0003-9861(74)90416-0

Cited by 27 publications

(18 citation statements)

References 17 publications

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“…In the present study, the malate transport system was found to be constitutive, since the levels of [14C]malate uptake by wild-type cells grown in the glucose-containing minimal medium with and without malate appeared to be the same. This result was in contrast to the results for many fungal and bacterial systems (5,8,10,11,12,13). The inducible malate transport systems in K. lactis, Saccharomyces bailii, and N. crassa have been found to be repressed by glucose, fructose, and sucrose, respectively (2,20,22).…”

Section: Resultscontrasting

confidence: 83%

Malate transport in Schizosaccharomyces pombe

Osothsilp¹,

Subden²

1986

J Bacteriol

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The transport of malate was studied in a Schizosaccharomyces pombe wild-type strain and in mutant strains unable to utilize malic acid. Two groups of such mutants, i.e., malic enzyme-deficient and malate transportdefective mutants, were differentiated by a '4C-labeled L-malate transport assay and by starch gel electrophoresis followed by activity staining for malic enzyme (malate dehydrogenase [oxaloacetate decarboxylating] [NAD+]; 1.1.1.38) and malate dehydrogenase (1.1.1.37). Transport of malate in S. pombe was constitutive and strongly inhibited by inhibitors of oxidative phosphorylation and of the formation of proton gradients.Transport was a saturable function of the malate concentration. The apparent Km and V. values for transport by the parent were 3.7 mM and 40 nmol/min per mg of protein, respectively, while those of the malic enzyme-deficient mutant were 5.7 mM and 33 nmol/min per mg of protein, respectively. Malate transport was pH and temperature dependent. The specificity of transport was studied with various substrates, including mono-and dicarboxylic acids, and the possibility of a common transport system for dicarboxylic acids is discussed.The ability of Schizosaccharomyces pombe to metabolize malic acid has been known for some time (A. Y. Kluyver, Ph.D. thesis,

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Section: Resultscontrasting

confidence: 83%

Malate transport in Schizosaccharomyces pombe

Osothsilp¹,

Subden²

1986

J Bacteriol

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“…All these results give evidence for a functional C,-dicarboxylic acid transport system in free-living R. meliloti. Dicarboxylate transport systems in free-living cells have been shown in E. coli (Kay & Kornberg, 1971 ;Lo et al, 1972), Bacillus subtilis (Ghei & Kay, 1973), Azotobacter vinelandii (Reuser & Postma, 1973), Pseudomonasputida (Dubler et al, 1974), Salmonella typhimurium (Kay & Cameron, 1978), 'R. trifolii' (Ronson et al, 1981), R. leguminosarum Finan et al, 1981) and Bradyrhizobium japonicum (McAllister & Lepo, 1983 ;San Francisco & Jacobson, 1985).…”

Section: Discussionmentioning

confidence: 99%

Biochemical and Genetical Analysis of Rhizobium meliloti Mutants Defective in C4-dicarboxylate Transport

Engelke¹,

Jagadish²,

Pühler³

1987

Microbiology

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Inhibition studies with free-living cells of Rhizobium meliloti 201 1 showed that succinate, fumarate and malate are transported via a common transport system. It is an active process that is inducible by succinate. The apparent K,,, for succinate uptake in free-living cells is 5.3 p~. Seven Tn.5-induced mutants that did not grow on succinate, fumarate or malate lacked the C4-dicarboxylate transport system in the free-living state (Dctfl-). Five of these mutants (RMS11, RMS16, RMS17, RMS24, RMSl18) induced nodules on alfalfa with about half the nitrogenfixation activity of the wild-type (Fixred). The remaining two mutants (RMS420 and RMS938) gave rise to small, white and ineffective nodules on alfalfa (Fix-). These results correlated with the dicarboxylate transport rates of bacteroids isolated from appropriate nodules : bacteroids isolated from nodules induced by the Fixred mutants transported succinate or malate at about 30-50% of the wild-type rate (Dctsred), whereas bacteroids isolated from nodules induced by Fixmutants showed no uptake activity (Dcts-). From an R. meliloti 201 1 bank three cosmids were identified which complemented different Dctfl-mutants. Complemented Dctfl-mutants could grow again on agar containing succinate or malate. In the case of Fix-mutants complementation resulted in effective nodules. The cosmids pRmSC 101 and pRmSClO2 complemented the Fix-mutant RMS420. A third cosmid, pRmSC121, complemented the second Fix-mutant RMS938 and nearly all of the Fixred mutants. For the Fix-mutant RMS420 the Tn5 insertion site was found to be located on a 15-3 EcoRI fragment which is also part of the complementing cosmid pRmSC102.

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“…The C4-dicarboxylates are transported by a common system in several bacterial species (e.g., see refs. [18][19][20], including R. leguminosarum (7,9). The inhibition of succinate uptake by fumarate and malate and the inability ofthe mutant strains to grow on or transport succinate, fumarate, or malate indicates that they are also transported by a common system in R. trtifolii strain 7012.…”

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confidence: 99%

C ₄ -dicarboxylate transport mutants of Rhizobium trifolii form ineffective nodules on Trifolium repens

Ronson¹,

Lyttleton

Robertson³

1981

Proc. Natl. Acad. Sci. U.S.A.

231

156

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Mutants of Rhizobium trifolii strain 7012 defective in C4-dicarboxylate transport were isolated by using a selective procedure based on pH indicator media. The mutant strains CR7098 and CR7099 failed to grow on or transport succinate, fumarate, or malate, but grew at wild-type rates on several other carbon sources.The C4-dicarboxylate transport system was inducible in strain 7012, but was expressed constitutively in four out of five succinate-positive revertants of strain CR7098. In the fifth CR7098 revertant (strain CR8008) the system was inducible. However, in contrast to strain 7012, strain CR8008 failed to use the C4-dicarboxylates in the presence of a second carbon source. Revertants of strain CR7099 were similar to strain 7012. Both strains CR7098 and CR7099 nodulated white and red clover at a rate similar to that ofstrain 7012, but nodules formed by the mutant strains were white and ineffective. Microscopic examination showed that the pattern of development of white clover nodules formed by strain CR7098 was similar to that observed with nodules formed by strain 7012, except that large amounts of starch accumulated in bacteroid-filled cells and senescence occurred earlier. Revertant strains were effective, except for strain CR8008, which formed ineffective nodules. The results show that a supply of C4-dicarboxylates to bacteroids is essential for nitrogen fixation in clover nodules. However, rhizobia within plant cells must also utilize other carbon sources to support growth and division.

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Transport of succinate by pseudomonas putida

Cited by 27 publications

References 17 publications

Malate transport in Schizosaccharomyces pombe

Malate transport in Schizosaccharomyces pombe

Biochemical and Genetical Analysis of Rhizobium meliloti Mutants Defective in C4-dicarboxylate Transport

C ₄ -dicarboxylate transport mutants of Rhizobium trifolii form ineffective nodules on Trifolium repens

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Transport of succinate by pseudomonas putida

Cited by 27 publications

References 17 publications

Malate transport in Schizosaccharomyces pombe

Malate transport in Schizosaccharomyces pombe

Biochemical and Genetical Analysis of Rhizobium meliloti Mutants Defective in C4-dicarboxylate Transport

C 4 -dicarboxylate transport mutants of Rhizobium trifolii form ineffective nodules on Trifolium repens

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C ₄ -dicarboxylate transport mutants of Rhizobium trifolii form ineffective nodules on Trifolium repens