Transport of membrane receptors and the mechanics of sexual cell fusion in <i>Chlamydomonas eugametos</i>

Homan, Wieger; Sigon, Corrien A.M.; Briel, Wies van den; Wagter, Ron; Nobel, Hans de; Mesland, D. A. M.; Musgrave, A.; Ende, H. van den

doi:10.1016/0014-5793(87)80170-9

Cited by 32 publications

(23 citation statements)

References 10 publications

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“…The tipping of cross-linked surface proteins is displayed by both vegetative (mitotic) and gametic cells of Chlamydomonas [Goodenough and Jurivich, 1978;Bloodgood et al, 1986;Homan et al, 1987Homan et al, , 1988Kooijman et al, 1989, 19901. In addition, gametes display a ''natural" tipping response.…”

Section: Introductionmentioning

confidence: 98%

Tipping of flagellar agglutinins by gametes of Chlamydomonas reinhardtii

Goodenough

1993

Cell Motil. Cytoskeleton

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The migration of cross-linked agglutinins to the gametic flagellar tips (tipping) is a hallmark of the Chlamydomonas mating reaction. In this study, an assay was developed to analyze the kinetics and biological requirements for the tipping response: isolated flagella from mt- gametes of C. reinhardtii were allowed to agglutinate to the immotile flagella of pf-18 mt+ gametes, and their migration to the tips was monitored by phase microscopy. The tipping process is shown to require both adhesion and elevated levels of cAMP. The cAMP may activate tipping motors directly. In addition, cAMP stimulates the recruitment of agglutinins to the flagellar surface to replace those inactivated by adhesion. These results are compared with previous studies on the tipping of flagellar surface proteins cross-linked by soluble ligands, and an integrated model is presented.

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Section: Introductionmentioning

confidence: 98%

Tipping of flagellar agglutinins by gametes of Chlamydomonas reinhardtii

Goodenough

1993

Cell Motil. Cytoskeleton

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“…Bloodgood (1992) suggested that a calcium-stimulated protein kinase activity in agella of C. reinhardtii is involved in this mechanism. Since transport of agglutinins is an important sexual response for properly aligning gametes for cell fusion (Goodenough and Jurivich 1978;Homan et al 1987), we set out to identify comparable kinase activity in C. moewusii gametes. Here we describe the characterisation of a calcium-stimulated protein kinase in the Chlamydomonas¯agellum that is similar to calmodulin-like domain protein kinases (CDPKs; Roberts and Harmon 1992).…”

Section: Introductionmentioning

confidence: 99%

Characterisation and cloning of a calmodulin-like domain protein kinase from Chlamydomonas moewusii (Gerloff )

Siderius

Henskens

Porto-Leblanche

et al. 1997

Planta

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General rights It is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. Download date: 12 May 2018Abstract. Calcium-stimulated protein kinase activity in the¯agella of the green alga Chlamydomonas moewusii (Gerlo ) was characterised. Using SDS-PAGE and an on-blot phosphorylation assay, a 65-kDa protein was identi®ed as the major calcium-stimulated protein kinase. Its activity was directly stimulated by calcium, a characteristic of the calmodulin-like domain protein kinases (CDPKs). Monoclonal antibodies raised against the CDPKa from soybean cross-reacted with the 65-kDa protein in the¯agella, and also with other proteins in thē agellum and cell body. The same monoclonal antibodies were used to screen a C. moewusii cDNA expression library in order to isolate CDPK cDNAs from C. moewusii. The CCK1 cDNA encodes a protein with a kinase and calmodulin-like domain linked by a junction domain typical of CDPKs. From Southern analyses, evidence was obtained for a CDPK gene family in C. moewusii and C. reinhardtii.

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“…(1) flagellar beating changes from a swimming stroke into a twitching movement (Homan et al 1980); (2) flagellar-tip activation (FTA) occurs, which is the rounding of the flagellar tip as a consequence of the elongation of some outer microtubules (Mesland et al 1980;Elzenga et al 1982;Crabbendam et al 1984); (3) there is a transient increase in agglutinability (Demets et al 1988); (4) there is transport of agglutinins to the flagellar tip (tipping; Homan et al 1987); (5) local cell-wall lysis occurs between the bases of the flagella and a plasma papilla protrudes through the anterior cell wall, allowing the gamete to fuse with its partner. The dynamics of these mating responses and their role in mating have been described by Musgrave and van den Ende (1987).…”

Section: Introductionmentioning

confidence: 99%

Cyclic AMP is one of the intracellular signals during the mating of Chlamydomonas eugametos

Kooijman

Wildt

Briel

et al. 1990

Planta

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Chlamydomonas eugametos gametes of opposite mating type make cell-cell contact via their flagellar surfaces. This contact triggers an increase in the intracellular level of cyclic AMP (cAMP) and several cellular responses which are necessary for cell fusion. Here, we show that wheat-germ agglutinin, which binds to the flagellar surface and induces all mating responses, also increased the intracellular cAMP level. Dibutyryl-cAMP added to non-mating gametes induced flagellar twitching, cell-wall lysis, mating-structure activation, flagellartip activation and an increase in agglutinability. It did not induce agglutinin transport to the flagellar tip (tipping) and may not be the direct cause of flagellar twitching and flagellar-tip activation. In non-illuminated cells, dibutyryl-cAMP was far more effective in evoking mating reactions than in illuminated cells. Light induced a 50% decrease in the cAMP level within 1 min. Adenylate cyclase was found to be associated with cell membranes but only 8% of the total was present in the gamete flagella.

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Transport of membrane receptors and the mechanics of sexual cell fusion in Chlamydomonas eugametos

Cited by 32 publications

References 10 publications

Tipping of flagellar agglutinins by gametes of Chlamydomonas reinhardtii

Tipping of flagellar agglutinins by gametes of Chlamydomonas reinhardtii

Characterisation and cloning of a calmodulin-like domain protein kinase from Chlamydomonas moewusii (Gerloff )

Cyclic AMP is one of the intracellular signals during the mating of Chlamydomonas eugametos

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